Pelargonium flower-break and pelargonium line pattern viruses in the Netherlands; purification, antiserum preparation, serological identification, and detection in pelargonium by ELISA

Bouwen, I.; Maat, D. Z.

doi:10.1007/bf01996326

Cited by 18 publications

(14 citation statements)

References 16 publications

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“…throughout the world (1,3,6,19,41,52). Its most conspicuous symptoms are white flower streaking, chlorotic spotting of leaves, and growth reduction causing significant losses in crop production.…”

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confidence: 99%

Insights into the Selective Pressures Restricting Pelargonium Flower Break Virus Genome Variability: Evidence for Host Adaptation

Rico

Ivars

Elena

et al. 2006

J Virol

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The molecular diversity of Pelargonium flower break virus (PFBV) was assessed using a collection of isolates from different geographical origins, hosts, and collecting times. The genomic region examined was 1,828 nucleotides (nt) long and comprised the coding sequences for the movement (p7 and p12) and the coat (CP) proteins, as well as flanking segments including the entire 3 untranslated region (3 UTR). Some constraints limiting viral heterogeneity could be inferred from sequence analyses, such as the conservation of the amino acid sequences of p7 and of the shell domain of the CP, the maintenance of a leucine zipper motif in p12, and the preservation of a particular folding in the 3 UTR. A remarkable covariation, involving five specific amino acid sites, was found in the CP of isolates largely propagated in the local lesion host Chenopodium quinoa and in the progeny of a PFBV variant subjected to serial passages in this host. Concomitant with this covariation, up to 30 nucleotide substitutions in a 1,428-nt region of the viral RNA could be attributable to C. quinoa-specific adaptation, representing one of the most outstanding cases of host-driven genome variation for a plant virus. Globally, the results indicate that the selective pressures exerted by the host play a critical role in shaping PFBV populations and that these populations are likely being selected for at both protein and RNA levels.It is commonly believed that the high mutation rates characteristic of RNA viruses, together with their short replication times and large populations, favor their rapid adaptation to changing situations (12, 13). Despite the high potential for genetic variation of these pathogens, a considerable number of studies on the diversity of plant virus populations have found a remarkable genetic stability (reviewed in reference 21). This stability is usually considered to be the result of selective pressures inherently imposed by genome replication and expression strategies and by virus-vector and virus-host interactions. In addition, random genetic drift due to bottlenecks associated with transmission or colonization events may also limit the eventual accumulation of variation (21,23,47). Investigating the factors affecting the diversity levels of viral populations can undoubtedly provide significant clues for the development of efficient and stable control strategies for viral pathogens (22,27,49).Pelargonium flower break virus (PFBV) is one of the most prevalent viruses infecting geraniums (Pelargonium spp.) throughout the world (1, 3, 6, 19, 41, 52). Its most conspicuous symptoms are white flower streaking, chlorotic spotting of leaves, and growth reduction causing significant losses in crop production. PFBV is mainly transmitted by vegetative propagation and mechanical inoculation, although transmissions by irrigation systems and by the western flower thrips Frankiniella occidentalis may also occur (30).PFBV is a member of the genus Carmovirus (family Tombusviridae) and, like all the species in the group, produces icosahe...

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confidence: 99%

Insights into the Selective Pressures Restricting Pelargonium Flower Break Virus Genome Variability: Evidence for Host Adaptation

Rico

Ivars

Elena

et al. 2006

J Virol

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“…Plants are generally tested using ELISA in propagation nurseries, and this technique is reliable yearround (Bouwen and Maat 1992). A non-radioactive dot hybridisation assay has also been developed and successfully used to diagnose PFBV (Ivars et al 2004).…”

Section: Diagnostic Informationmentioning

confidence: 99%

A Photo-Based Key of Thrips (Thysanoptera) Associated with Horticultural Crops in Florida

Cluever

Smith

2017

Florida Entomologist

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“…Viruses infecting Pelargonium spp. have been described in Canada (Kemp, 1967), Spain (Pen˜a- Iglesias et al, 1974), Denmark (Paludan, 1976), Greece (Vovlas and Grigoriu, 1977), the United Kingdom (Stone, 1980), Belgium (Welvaert et al, 1982), The Netherlands (Bouwen and Maat, 1992) and Israel (Franck and Loebenstein, 1994). In SpainÕs temperate climate, geraniums are kept as perennials and can serve as reservoirs for new infections in both ornamental geraniums and horticultural plants.…”

Section: Introductionmentioning

confidence: 97%

High incidence of Pelargonium line pattern virus infecting asymptomatic Pelargonium spp. in Spain

Alonso¹,

Borja²

2005

Eur J Plant Pathol

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Geraniums (Pelargonium spp.) are traditional ornamental plants largely cultivated in Europe and northern America. Vegetative propagation makes them prone to viral infections, which have detrimental effects on crop production and quality. Asymptomatic samples collected in Spain were tested for a range of viruses using ELISA. The tobamovirus, Tobacco mosaic virus (TMV), the cucumovirus, Cucumber mosaic virus (CMV), and several viruses in the family Tombusviridae, namely, Pelargonium line pattern virus (PLPV), Pelargonium flower break virus (PFBV), and Pelargonium leaf curl virus (PLCV), were detected either singly or in combination in 59.2% of 800 samples. PLPV and PFBV infections were confirmed by dot-blot hybridisation. The most relevant viral infection found on Spanish asymptomatic geraniums was by Pelargonium line pattern virus (PLPV). Symptoms did not develop for 3 years on most of the PLPV infected geranium plants under greenhouse conditions.

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Pelargonium flower-break and pelargonium line pattern viruses in the Netherlands; purification, antiserum preparation, serological identification, and detection in pelargonium by ELISA

Cited by 18 publications

References 16 publications

Insights into the Selective Pressures Restricting Pelargonium Flower Break Virus Genome Variability: Evidence for Host Adaptation

Insights into the Selective Pressures Restricting Pelargonium Flower Break Virus Genome Variability: Evidence for Host Adaptation

A Photo-Based Key of Thrips (Thysanoptera) Associated with Horticultural Crops in Florida

High incidence of Pelargonium line pattern virus infecting asymptomatic Pelargonium spp. in Spain

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