High incidence of Pelargonium line pattern virus infecting asymptomatic Pelargonium spp. in Spain

Alonso, Mertxe; Borja, M.

doi:10.1007/s10658-005-0803-1

Cited by 14 publications

(6 citation statements)

References 15 publications

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“…Strikingly, PLPV usually causes asymptomatic infections in their natural hosts (Pelargonium spp.) as well as in experimental hosts, such as N. benthamiana or Nicotiana clevelandii (58,94). Moreover, PLPV titers are considerably low in infected tissue (65), which could suggest that either the virus lacks an efficient VSR or the expression/activity of such a VSR is modulated during infection.…”

Section: Discussionmentioning

confidence: 99%

Key Importance of Small RNA Binding for the Activity of a Glycine-Tryptophan (GW) Motif-containing Viral Suppressor of RNA Silencing

Pérez-Cañamás

Hernández

2015

Journal of Biological Chemistry

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Background: GW/WG motif-containing viral suppressors of RNA silencing (VSRs) have been proposed to act through interaction with Argonaute (AGO) proteins. Results: The activity of a GW motif-containing VSR was found to rely on small RNA binding capability rather than on AGO interaction. Conclusion:Overlapping signals in VSRs may lead to misinterpretation of relevant molecular traits. Significance: Knowing primary target(s) of VSRs is critical for better understanding of the host-virus arms race.

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Section: Discussionmentioning

confidence: 99%

Key Importance of Small RNA Binding for the Activity of a Glycine-Tryptophan (GW) Motif-containing Viral Suppressor of RNA Silencing

Pérez-Cañamás

Hernández

2015

Journal of Biological Chemistry

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“…To date, work performed on PLPV at the molecular level includes (i) sgRNA mapping [5], (ii) generation of infectious cDNA clones under the control of the T7 RNA polymerase promoter or 35S cauliflower mosaic virus promoter [6], (iii) analyses of gene function and translational mechanisms [8], (iv) analyses of molecular variability [7], and (v) identification of the VSR and its molecular interactions [26]. Though limited, data on the incidence of PLPV in cultivated geraniums in Spain have been also obtained [2].…”

Section: Introductionmentioning

confidence: 99%

Pelarspovirus, a proposed new genus in the family Tombusviridae

et al. 2015

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Currently, the family Tombusviridae encompasses thirteen viral genera that contain single-stranded, positive-sense RNA genomes and isometric virions; the exception being the genus Umbravirus, whose members do not encode a coat protein (CP). A new genus, tentatively named Pelarspovirus, is proposed to be added to this family and would include five members, with Pelargonium line pattern virus recommended as the type species. Viruses assigned to this proposed genus have monopartite genomes encoding five open reading frames (ORFs) that include two 5 0 -proximal replication proteins, two centrally located movement proteins (MP1 and MP2) and a 3 0 -proximal CP that, at least for pelargonium line pattern virus (PLPV), has been shown to act also as suppressor of RNA silencing. Distinguishing characteristics of these viruses include i) production of a single, tricistronic subgenomic RNA for expression of MP and CP genes, ii) presence of a non-AUG start codon (CUG or GUG) initiating the MP2 ORF, iii) absence of AUG codons in any frame between the AUG initiation codons of MP1 and CP genes, and iv) sequence-based phylogenetic clustering of all encoded proteins in separate clades from those of other family members.

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“…throughout the world (1,3,6,19,41,52). Its most conspicuous symptoms are white flower streaking, chlorotic spotting of leaves, and growth reduction causing significant losses in crop production.…”

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Insights into the Selective Pressures Restricting Pelargonium Flower Break Virus Genome Variability: Evidence for Host Adaptation

Rico

Ivars

Elena

et al. 2006

J Virol

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The molecular diversity of Pelargonium flower break virus (PFBV) was assessed using a collection of isolates from different geographical origins, hosts, and collecting times. The genomic region examined was 1,828 nucleotides (nt) long and comprised the coding sequences for the movement (p7 and p12) and the coat (CP) proteins, as well as flanking segments including the entire 3 untranslated region (3 UTR). Some constraints limiting viral heterogeneity could be inferred from sequence analyses, such as the conservation of the amino acid sequences of p7 and of the shell domain of the CP, the maintenance of a leucine zipper motif in p12, and the preservation of a particular folding in the 3 UTR. A remarkable covariation, involving five specific amino acid sites, was found in the CP of isolates largely propagated in the local lesion host Chenopodium quinoa and in the progeny of a PFBV variant subjected to serial passages in this host. Concomitant with this covariation, up to 30 nucleotide substitutions in a 1,428-nt region of the viral RNA could be attributable to C. quinoa-specific adaptation, representing one of the most outstanding cases of host-driven genome variation for a plant virus. Globally, the results indicate that the selective pressures exerted by the host play a critical role in shaping PFBV populations and that these populations are likely being selected for at both protein and RNA levels.It is commonly believed that the high mutation rates characteristic of RNA viruses, together with their short replication times and large populations, favor their rapid adaptation to changing situations (12, 13). Despite the high potential for genetic variation of these pathogens, a considerable number of studies on the diversity of plant virus populations have found a remarkable genetic stability (reviewed in reference 21). This stability is usually considered to be the result of selective pressures inherently imposed by genome replication and expression strategies and by virus-vector and virus-host interactions. In addition, random genetic drift due to bottlenecks associated with transmission or colonization events may also limit the eventual accumulation of variation (21,23,47). Investigating the factors affecting the diversity levels of viral populations can undoubtedly provide significant clues for the development of efficient and stable control strategies for viral pathogens (22,27,49).Pelargonium flower break virus (PFBV) is one of the most prevalent viruses infecting geraniums (Pelargonium spp.) throughout the world (1, 3, 6, 19, 41, 52). Its most conspicuous symptoms are white flower streaking, chlorotic spotting of leaves, and growth reduction causing significant losses in crop production. PFBV is mainly transmitted by vegetative propagation and mechanical inoculation, although transmissions by irrigation systems and by the western flower thrips Frankiniella occidentalis may also occur (30).PFBV is a member of the genus Carmovirus (family Tombusviridae) and, like all the species in the group, produces icosahe...

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High incidence of Pelargonium line pattern virus infecting asymptomatic Pelargonium spp. in Spain

Cited by 14 publications

References 15 publications

Key Importance of Small RNA Binding for the Activity of a Glycine-Tryptophan (GW) Motif-containing Viral Suppressor of RNA Silencing

Key Importance of Small RNA Binding for the Activity of a Glycine-Tryptophan (GW) Motif-containing Viral Suppressor of RNA Silencing

Pelarspovirus, a proposed new genus in the family Tombusviridae

Insights into the Selective Pressures Restricting Pelargonium Flower Break Virus Genome Variability: Evidence for Host Adaptation

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