Pectinase production by Aspergillus niger LB-02-SF is influenced by the culture medium composition and the addition of the enzyme inducer after biomass growth

Reginatto, Caroline; Rossi, Caroline; Miglioranza, Betina Gamba; Santos, Marielem dos; Meneghel, Lenara; Silveira, Maurício Moura da; Malvessi, Eloane

doi:10.1016/j.procbio.2017.04.018

Cited by 26 publications

(20 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, higher fungal pectinase activities have been reported in the literature; for instance, Penicillium verruculosum reached 10.32 U mL -1 in culture media supplemented with orange peel (Stroparo et al 2012), and Aspergillus niger LB-02-SF exhibited 14 U mL -1 , when pectin was added to the medium (Reginatto et al 2017). Despite this, several pectinases have been hitherto reported for A. japonicus, such as endopolygalacturonases and pectinesterases (Hasunuma et al 2003, Semenova et al 2003, Teixeira et al 2011, pectin lyases (Semenova et al 2003, Ishii & Yokotsuka 1975, and exo-polygalacturonases (Teixeira et al 2011), indicating that this species can be highly promising for the production of these enzymes.…”

Section: Enzymesmentioning

confidence: 99%

Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1

Ferreira

Dall'Antonia

Shiga

et al. 2018

Hoehnea

View full text Add to dashboard Cite

-(Sugarcane bagasse as a source of carbon for enzyme production by fi lamentous fungi). The aim of the present work was to assess the enzymatic activity of six strains of fi lamentous fungi grown in liquid media containing 1% sugarcane bagasse as the sole carbon source. All fungal strains were able to use this agro-industrial residue, producing various types of enzymes, such as cellulases, xylanases, amylases, pectinases, and laccases. However, Aspergillus japonicus Saito was the most effi cient producer, showing the highest enzymatic activity for laccase (395.73 U L -1 ), endo-β-1,4-xylanase (3.55 U mL -1 ) and β-xylosidase (9.74 U mL -1 ) at seven, fourteen and twenty-one days in culture, respectively. Furthermore, the endo-β-1,4-xylanases and β-xylosidases of A. japonicus showed maximum activity at 50°C, and pH 5.5 and pH 3.5-4.5, respectively. Thus, these results indicate that A. japonicus has a great biotechnological potential for the production of these enzymes using sugarcane bagasse as the sole source of carbon.

show abstract

Section: Enzymesmentioning

confidence: 99%

Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1

Ferreira

Dall'Antonia

Shiga

et al. 2018

Hoehnea

View full text Add to dashboard Cite

show abstract

“…DT7, Aspergillus niger LB-02-SF, Streptomyces sp. P12-137, have been used for the separate production of xylanase and pectinase enzymes from such crude polysaccharide substrates [21][22][23][24] . Thus, it was necessary to screen these substrates for efficient concurrent induction of xylanase and pectinase enzymes.…”

Section: Resultsmentioning

confidence: 99%

“…In literature, B. pumilus AJK has been reported for the production of xylanase and pectinase after growing on 2% WB and 2% Mosambi Peels 11 . Enhancement of pectinase activities by Aspergillus niger LB-02-SF has been reported after the supplementation of wheat bran to the orange residues containing media 23 .…”

Section: Effect Of Simultaneous Supplementation Of Wb and Cp On Concumentioning

confidence: 99%

Optimization of concurrent production of xylanolytic and pectinolytic enzymes by Bacillus safensis M35 and Bacillus altitudinis J208 using agro-industrial biomass through Response Surface Methodology

Thite

Nerurkar

Baxi

2020

Sci Rep

View full text Add to dashboard Cite

Application of crude xylanolytic and pectinolytic enzymes in diverse industrial processes make these enzymes commercially valuable and demand their production process to be cost-effective. Out of four different agrowaste biomass, wheat bran (WB) and citrus peel (CP), when amended as fermentation substrates, respectively induced the highest xylanolytic enzymes and pectinolytic enzymes from both, B. safensis M35 and B. altitudinis J208. Further, the simultaneous amendment of WB and CP yielded concurrent production of these cellulase free xylanolytic and pectinolytic enzymes. Hence, the quadratic model was developed using the Central Composite Design of Response Surface Method (CCD-RSM). The model gave the concentration values for WB and CP substrates to be amended in one single production medium for obtaining two optimized predicted response values of xylanase activity and pectinase activity units, which were further practically validated for the xylanase and pectinase production responses from the optimized production medium (OPM). These practically obtained response values from OPM were found to be in accordance with a range of 95% predicted intervals (PI) values. These observations verified the validity of the predicted quadratic model from RSM and suggested that both xylanase and pectinase enzymes can be induced concurrently from both of the bacterial strains. Xylanases and pectinases are the commercially important industrial groups of enzymes, members of which exhibit diverse xylanolytic and pectinolytic enzymatic activities. These groups of enzymes harbor a huge commercial potential as their biotechnological applications span broad spectra in diverse industries such as biofuels, pulp-paper, food, animal feed, textile, fiber, etc. Out of these, biofuel industries demand these xylanolytic and pectinolytic enzymes play their accessory role to the core cellulase enzymes for improving plant biomass saccharification. Whereas, animal feed industries require combination of cellulase, xylanase and or pectinase for improving the nutrition quality of grain and feed. On the other hand, pulp and paper as well as textile industries need the cellulase free xylanase and pectinase enzymes for their respective applications, i.e., to prepare hemicellulose free cellulose papers, to remove pectin (rhamnogalacturonan) coating from cotton and denim fibers, Food and beverage industries also need xylanase and pectinase enzymes to clarify pectin polymeric fraction from fruit juices and to improve the tea flavor 1,2. These applications append the worth to the organisms which can produce the xylanase and pectinase enzymes and the cellulase free nature of such enzymes is an add-on benefit to this.

show abstract

“…While the commercial availability of A. niger pectinase cocktails indicates that the industry has established strains for pectinase production, almost no information on their specific origin is publically accessible. In the academic field, numerous studies on optimization of fermentation conditions for pectinase production with various strains have been published (e.g., [1,18,42]). However, these do not allow for direct performance comparison of individual strains due to varying study designs and fermentation conditions.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Comparative evaluation of Aspergillus niger strains for endogenous pectin-depolymerization capacity and suitability for d-galacturonic acid production

Schäfer

Schmitz

Weuster‐Botz

et al. 2020

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

Pectinaceous agricultural residues rich in d-galacturonic acid (d-GalA), such as sugar beet pulp, are considered as promising feedstocks for waste-to-value conversions. Aspergillus niger is known for its strong pectinolytic activity. However, while specialized strains for production of citric acid or proteins are well characterized, this is not the case for the production of pectinases. We, therefore, systematically compared the pectinolytic capabilities of six A. niger strains (ATCC 1015, ATCC 11414, NRRL 3122, CBS 513.88, NRRL 3, and N402) using controlled batch cultivations in stirred-tank bioreactors. A. niger ATCC 11414 showed the highest polygalacturonase activity, specific protein secretion, and a suitable morphology. Furthermore, d-GalA release from sugar beet pulp was 75% higher compared to the standard lab strain A. niger N402. Our study, therefore, presents a robust initial strain selection to guide future process improvement of d-GalA production from agricultural residues and identifies a high-performance base strain for further genetic optimizations.

show abstract

Pectinase production by Aspergillus niger LB-02-SF is influenced by the culture medium composition and the addition of the enzyme inducer after biomass growth

Cited by 26 publications

References 34 publications

Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1

Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1

Optimization of concurrent production of xylanolytic and pectinolytic enzymes by Bacillus safensis M35 and Bacillus altitudinis J208 using agro-industrial biomass through Response Surface Methodology

Comparative evaluation of Aspergillus niger strains for endogenous pectin-depolymerization capacity and suitability for d-galacturonic acid production

Contact Info

Product

Resources

About