Pecan Pollen Stored Over a Decade Retains Viability

Sparks, Darrell; Yates, I.E.

doi:10.21273/hortsci.37.1.176

Cited by 15 publications

(5 citation statements)

References 4 publications

(6 reference statements)

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“…Since the last century, various methods for storing pollen have been tested in different species (Barnabas & Rajki 1981;Barnabas 1984;Yates et al 1991;Connor & Towill 1993;Rajasekharan et al 1994;Spark & Yates 2002), proving that temperature and moisture content are important factors that influence its preservation. It has been possible to store Zea mays L. pollen under cryogenic temperatures when the water content, in relationship to the fresh weight, is reduced to 30% of the original water content (Barnabas & Rajki 1981;Barnabas 1984).…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, it has been possible to store Carya illinoensis (Wangenh.) C. Koch pollen at -196°C for a period of 13 years without any effects on viability, and storage has been successful at -12°C for 2 years following a drying process at constant weight (Yates et al 1991;Connor & Towill 1993;Rajasekharan et al 1994;Spark & Yates 2002). Additionally, pollen from Gladiolus sp., an important ornamental plant, was kept viable for a period of 10 years under cryogenic temperatures (Rajasekharan et al 1994).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of temperature and drying on the longevity of storedNothofagus alpinapollen

Cruzatty

Rivero²,

Droppelmann

2015

New Zealand Journal of Botany

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Long-term pollen storage is important so that producers and breeders may be able to utilise stored pollen to cross individuals that bloom at different times or in different places. The objective of the current study was to determine the moisture content and optimal temperature for the storage of Nothofagus alpina (P. et E.) Oerst pollen. Pollen samples used for this study were obtained from established trees at the 'Huillilemu' clonal seed orchard, located in the commune of San José de la Mariquina, in southwestern Chile. Pollen extraction was conducted by placing it in a climatic chamber (25°C and 50% relative humidity) for 24 hours. The obtained pollen was subjected to various drying times and stored at three temperatures: 4°C; -13°C; and -80°C. Longevity of pollen stored at 4°C and -13°Cwas short, although it was possible to increase longevity by storing it at -80°C.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of temperature and drying on the longevity of storedNothofagus alpinapollen

Cruzatty

Rivero²,

Droppelmann

2015

New Zealand Journal of Botany

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“…This event was reported for other species such as Prunus nume [ 83 ], pineapple [ 78 ], Solanum spp. [ 65 ], pecan [ 87 ], mango, and litchi pollen [ 88 ]. This increase can be attributed to the very low temperature or/and the dehydration that, in combination or individually, contributed to the breakdown of the grain dormancy with an improvement in the percentage of vitality and germination [ 78 , 83 ].…”

Section: Resultsmentioning

confidence: 99%

Long-Term Conservation for the Safeguard of Abies nebrodensis: An Endemic and Endangered Species of Sicily

Benelli,

Tarraf,

İzgü

et al. 2024

Plants

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The combined approaches between ex situ and in situ conservation are of great importance for threatened species in urgent need of protection. This study aims to develop concrete actions to preserve the relic of 30 adult trees of the Sicilian fir (Abies nebrodensis) from extinction using long-term germplasm conservation in liquid nitrogen (LN, −196 °C). Pollen grains were collected, and their moisture content (MC) was measured. Then, viability (2,3,5-tryphenyl tetrazolium chloride, TTC), in vitro germinability, and enzymatic antioxidant activity (ascorbate peroxidase, APX; catalase, CAT) were evaluated before and after cryopreservation. Seeds collected from mature cones underwent X-ray analysis, and only full seeds were used to excise the zygotic embryos (ZEs) for cryopreservation. The MC percentage of ZEs was determined, and then they were plunged in LN with (+PVS2) or without (−PVS2) Plant Vitrification Solution 2; untreated ZEs were used as a control. Viability (TTC test) and in vitro germination were assessed for all ZEs (+PVS2, −PVS2, and control). Embryogenic callus (EC) lines obtained from mature ZEs were cryopreserved applying the ‘encapsulation-dehydration’ technique. This study has allowed, after optimizing cryopreservation protocols for pollen, ZEs, and EC of A. nebrodensis, to establish the first cryobank of this endangered species in Polizzi Generosa (Palermo, Italy), inside the ‘Madonie Regional Park’. The strategy developed for Sicilian fir conservation will pave the way for similar initiatives for other critically endangered conifer species.

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“…The maintenance of pollen viability using ultra-low temperature preservation has been realized in many species. It was reported that the viability of 8–10-years cryopreserved pollen from 12 species/cultivars of ornamental plants was higher than the fresh pollen, 17 species/cultivars retained the same viability as the control, and the viability of pecan pollen was still significantly higher than that of fresh pollen after cryopreservation (preservation in LN) of 13 years (Sparks and Yates, 2002 ; Ren et al, 2019 ). In maize, there was no significant difference in pollen pollination capacity between 1- and 2-years cryopreserved pollen and the control (Shi et al, 1996 ).…”

Section: Discussionmentioning

confidence: 99%

Improving Ultra-Low Temperature Preservation Technologies of Soybean Pollen for Off-Season and Off-Site Hybridization

et al. 2022

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Preserving viable pollen is of great interest to breeders to maintain desirable germplasm for future inbreeding. Ultra-low temperature preservation of pollen is an effective and safe way for long-term storage of plant germplasm resources. In this study, we improved methods for the preservation of soybean pollen at ultra-low temperature. Soybean flowers at the initially-open stage were collected at 6–10 a.m. during the fully-bloom stage of soybean plants and were dehydrated for 10 h and then frozen and stored at −196 or −80°C. In vitro culture experiments showed that the viability of preserved pollen remained as high as about 90%. The off-season (local site Heihe) and off-site (Beijing, after long-distance express delivery from Heihe) hybridization verification was conducted, and no significant difference in true hybrid rate was founded between the preserved pollen and the fresh pollen. The ultra-low temperature preservation technology for soybean pollen could break the spatiotemporal limit of soybean hybridization and facilitate the development of engineered soybean breeding.

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Pecan Pollen Stored Over a Decade Retains Viability

Cited by 15 publications

References 4 publications

Effect of temperature and drying on the longevity of storedNothofagus alpinapollen

Effect of temperature and drying on the longevity of storedNothofagus alpinapollen

Long-Term Conservation for the Safeguard of Abies nebrodensis: An Endemic and Endangered Species of Sicily

Improving Ultra-Low Temperature Preservation Technologies of Soybean Pollen for Off-Season and Off-Site Hybridization

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