Effect of temperature and drying on the longevity of stored<i>Nothofagus alpina</i>pollen

Cruzatty, Luz García; Rivero, Magaly; Droppelmann, Fernando

doi:10.1080/0028825x.2015.1045528

Cited by 5 publications

(13 citation statements)

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“…Se logró prolongar la viabilidad del polen de O. pyramidale reduciendo el contenido de humedad por debajo del 5 %, independientemente de las temperaturas de almacenamiento; bajo estas condiciones, prácticamente, no existe agua libre para el transporte intracelular (Shivanna y Jhori 1985, García et al 2015. Por otra parte, un elevado contenido de humedad permite que continúe la actividad metabólica y se pierda la viabilidad a medida que transcurre el tiempo de almacenamiento.…”

Section: Discussionunclassified

“…Contenido de humedad y almacenamiento del polen. Para la extracción y manejo del polen se siguió con la metodología de García et al (2015), aunque se probaron diferentes tiempos y temperatura de secado. Para calcular el Cuadro 1.…”

Section: Formulación Y Evaluación De Los Medios De Germinaciónunclassified

“…Tanto para calcular el contenido de agua del polen fresco, como para calcular la pérdida de agua después de la deshidratación, el polen fue pesado utilizando una balanza analítica, antes y después del secado. El contenido de agua de una muestra se calculó a partir de la pérdida de peso, teniendo como base su peso fresco (García et al 2015). Para el ensayo de almacenamiento, se mezcló el polen extraído para obtener una muestra homogénea.…”

Section: Sacarosaunclassified

“…Desde el siglo pasado, se han probado varios métodos para almacenar polen en diferentes especies (Yates et al 1991, Connor y Towill 1993, Rajasekharan et al 1994, García et al 2015 demostrando que la temperatura y el contenido de humedad son factores importantes que influyen en su conservación. Por ejemplo, ha sido posible prolongar la viabilidad del polen disminuyendo su contenido de humedad y bajo condiciones de crioconservación en varias especies, por ejemplo: Carya illinoensis (Wangenh.)…”

Section: Introductionunclassified

“…Por ejemplo, ha sido posible prolongar la viabilidad del polen disminuyendo su contenido de humedad y bajo condiciones de crioconservación en varias especies, por ejemplo: Carya illinoensis (Wangenh.) K. Koch (Yates et al 1991) (-196 °C), Nothofagus alpina (García et al 2015), algunas especies del género Pinus (Tighe 2004), especies del género Protea (Van der Walt y Littlejohn 1996). El contenido de agua es un parámetro que, de acuerdo con la comprensión actual, afecta directamente la viabilidad del polen.…”

Section: Introductionunclassified

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Condiciones óptimas para almacenamiento del polen de Ochroma pyramidale

et al. 2019

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Knowledge about pollen biology is fundamental for the planning of controlled crosses in programs of conservation and genetic improvement of plant species. The objectives of this research were to determine the ideal medium for in vitro germination of pollen and to evaluate the effect of temperature and drying on the viability of stored pollen from Ochroma pyramidale. Pollen was obtained from flowers collected before anthesis. The appropriate culture medium for in vitro germination of pollen was determined by testing modifications of the medium proposed by Brewbaker and Kwack. Pollen drying was performed during different times (60, 120, 180 and 240 minutes) at 29 °C and 45 % relative humidity. These samples were stored under temperatures of 5,-20 and-196 °C, and evaluated for 45 days. The ideal medium for pollen germination was composed of 10 % sucrose, 30 mg L-1 boric acid, 430 mg L-1 calcium nitrate. The initial viability calculated by in vitro germination was high, which indicates that the individuals evaluated can be used as pollen donors in the controlled crossing plans. Humidity below 5 % allowed keeping the pollen stored, independently of the storage temperature during the 45 days of the test, without significant decrease in their viability and germination rate. However, pollen viability decreased rapidly when it was stored with high moisture content, regardless of storage temperatures.

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Section: Discussionunclassified

Section: Formulación Y Evaluación De Los Medios De Germinaciónunclassified

Section: Sacarosaunclassified

Section: Introductionunclassified

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Condiciones óptimas para almacenamiento del polen de Ochroma pyramidale

et al. 2019

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Morphology, viability, and longevity of pollen of National Type and Trinitarian (CCN-51) clones of cocoa (Theobroma cacao L.) on the Coast of Ecuador

et al. 2019

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The objective of this study was to determine the ideal method for in vitro germinability of Theobroma cacao L. (Malvaceae) pollen, evaluate the initial viability and longevity, and describe its morphology. In order to gather information on the morphological and viability characteristics of pollen in Theobroma cacao, flowers were collected at noon. Viability was assessed by in vitro and in vivo germination and staining. The pollen of this species is small (20 μm) and spherical to prolate spheroidal in shape. The best way to evaluate the viability in vitro was using the sucrose and agar compound. Its initial viability through germination in vitro was between 56.6 and 86.6%; with the staining method it was 100%, and by germination in vivo 40.7-58.7%. There was a moderate correlation between germination in vitro and germination in vivo (r = 0.5), but no correlation between the viability calculated by the staining method and by germination in vivo; the latter method was recommended to perform evaluations of both viability and pollen longevity. In the clones of cocoa National type, pollen quickly loses its viability, and at 240 min there was no germination; nevertheless, the pollen of clone CCN-51 has a longevity of 24 h.

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New protocol for storage of viable pollen of Lapageria rosea (Philesiaceae), an endangered plant species endemic to temperate forests of Chile

Cruzatty

Droppelmann

Izaguirre‐Mayoral

2020

Plant Species Biology

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Lapageria rosea is an emblematic species, endemic to the temperate rainforest of Chile. As a result of extensive plantation of commercial exotic pine and eucalypts, scattered individuals of L. rosea are now encountered mainly in fragmented forest areas. Therefore, L. rosea has been declared in danger of extinction. However, the success of national conservation programs depends, in part, on the availability of stored viable pollen grains for pollinating assays, in order to sustain the genetic diversity of L. rosea offspring. Here we describe a simple and low-cost protocol for storing viable pollen grains for 1 year. The protocol involves oven-drying freshly collected pollen grains at 37 C for 15 min, followed by storage at-13 C. The stored pollen has an average of 60-65% in vitro germination after 5 months, with a reduction to 50% by the end of

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Effect of temperature and drying on the longevity of storedNothofagus alpinapollen

Cited by 5 publications

References 11 publications

Condiciones óptimas para almacenamiento del polen de Ochroma pyramidale

Condiciones óptimas para almacenamiento del polen de Ochroma pyramidale

Morphology, viability, and longevity of pollen of National Type and Trinitarian (CCN-51) clones of cocoa (Theobroma cacao L.) on the Coast of Ecuador

New protocol for storage of viable pollen of Lapageria rosea (Philesiaceae), an endangered plant species endemic to temperate forests of Chile

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