Pcr-rflp para identificação de ostras perlí­­feras (Pinctada imbricate) do Brasil e Venezuela

Abstract

PCR-RFLP PARA IDENTIFICAÇÃO DE OSTRAS PERLÍFERAS (Pinctada imbricate)DO BRASIL E VENEZUELA. RESUMOAs ostras do género Pinctada são de grande importância económica devido ao seu uso extensivo na alimentação humana e cultivo de pérolas. Inclui quatro espécies: Pinctada radiata (Europa), P. imbricata (Atlântico Ocidental) e P. fucatamartensi (Pacífico), sendo esta última uma espécie complexa de difícil diferenciação morfológica. Embora este complexo de espécies tenha vários estudos moleculares corroborando cada espécie como válida, ainda há dúvidas sobre a validade de P. imbricata no Sul do Atlântico Ocidental. Neste trabalho realializou-se um estudo de RFLP com populações do Ceará, Rio de Janeiro, São Paulo e costa da Venezuela. Foram analisados genes mitocondriais (16S) e nucleares (IGS parcial). Este estudo confirma que os espécimes brasileiros e venezuelanos são geneticamente mais próximos às populações de P. imbricata do Caribe do que o complexo P. martensi-fucata. Esse resultado é importante para os produtores de ostras perlíferas, demonstrando que os estoques brasileiro e venezuelano não são espécies exóticas ou híbridas de espécies indo-pacíficas. Palavras-chave:Ostras, Pinctada imbricata, América do Sul, ostra perlífera ABSTRACTOysters of the genus Pinctada are of great economic importance due to their extensive use in human feeding and pearl cultivation. It includes four species: Pinctada radiata (Europe), P. imbricata (Western Atlantic) and the P. fucata-martensi complex (Pacific), the latter being a species complex of difficult morphological differentiation. Although this species complex has several molecular studies corroborating each species as valid, there are still doubts about the validity of P. imbricata in the South of Western Atlantic (ie Brazilian Coast). Here we carried out a RFLP study with populations from Ceará, Rio de Janeiro, São Paulo and Venezuela coast. We analyzed mitochondrial (16S) and nuclear genes (partial IGS). This study confirms the Brazilian and Venezuelan stocks as genetically close to the P. imbricata stocks from Caribbean than the P. martensi-fucata complex. This result is important for pearl-oyster farmers, demonstrating that the Brazilian and Venezuelan stocks are not alien species or hybrids of Indo-Pacific species.

Identificação Molecular De Ostras Nativas Na Costa Do Maranhão, Brasil

“…The oysters of the genus Crassostrea (SACCO, 1897) are widely distributed and are found attached to substrates such as roots and rocks in the intertidal zone along the whole coast of Brazil (RIOS, 1994). The considerable morphological similarities of the different taxa hamper the reliable identification of these organisms, and contribute to widespread taxonomic uncertainties (RIOS, 1994;IGNACIO et al, 2000;BONDIOLI et al, 2017).…”

GENES DE VIRULENCIA E RESISTí­Å NCIA ANTIMICROBIANA DE Vibrio parahaemolyticus EM ÁREAS DE OSTREICULTURA

“…This is due to the proximity of natural banks in the town of Taperoá, a municipality with a population 40-times greater than that of the town of Torrinhas (E2) -a quilombola community composed of 450 inhabitants (IBGE, 2015). The lack of a sewage treatment system in most Brazilian municipalities directly affects the environmental quality of the estuaries due to the release of domestic wastes, as observed in the estuary of the river Una (SANTOS et al, 2015;BONDIOLI et al, 2017), which contributes to the proliferation and maintenance of Vibrio in the water due to the increase of debris and nutrients (COSTA et al, 2009).…”

GENETIC IMPLICATIONS OF RESTOCKING PROGRAMS ON WILD POPULATIONS OF STREAKED PROCHILOD <i>Prochilodus lineatus<i>

“…Genetic diversity levels and genetic composition of wild populations and farmed fish are fundamental to conservation strategies designed to improve the genetic composition of fish used in restocking projects (Bondioli et al, 2017;Duong and Scribner, 2018). However, reproduction within the confines of a commercial fish farm stands in stark contrast to the complexity of natural reproduction events that occur in large Neotropical rivers, especially in long-migration fish (Ribolli et al, 2016).…”