Identificação Molecular De Ostras Nativas Na Costa Do Maranhão, Brasil

Abstract

Oysters are found along the whole coast of Brazil. The phenotypes of the species vary considerably according to the characteristics of the habitat. The present study investigated the existence of different oyster species of the genus Crassostrea on the coast of Maranhão, using the Multiplex PCR technique and DNA Barcoding. The results of the Multiplex PCR revealed two distinct bands characteristic of the species C. gasar and C. rhizophorae in a total of 135 samples analyzed. The sequencing of the COI gene of 98 samples produced a 695 bp fragment and 15 haplotypes for C. gasar and 640 bp and eight haplotypes for C. rhizophorae. The haplotype tree divided the two species clearly into different clades with 100% bootstrap support. Intraspecific genetic divergence was 0.2% in both species, while interspecific divergence was 23.6%. The similarity between the sequences generated and those available in BoldSystems ranged from 97.01% to 98.37% for C. rhizophorae and from 97.55% to 99.84% for both C. gasar and C. brasiliana, reinforcing the taxonomic problems in this group, which supports the synonymization of these species. The DNA barcoding permitted the reliable identification of the samples and confirmed the existence of two species of oyster in the study area.

Recruitment of oyster in artificial collectors on the Amazon macrotidal mangrove coast

“…In the present research, the Multiplex PCR was able to identify the species Crassostrea gasar and Crassostrea rhizophorae in the same study area. The data reported are in agreement with other researches that show that in the Brazilian estuaries there are two or more species of oysters of the genus Crassostrea (LAPÈGUE et al, 2002;PIE et al, 2006;GARDUNHO et al, 2012;LOPES et al, 2018). The knowledge of the native oyster species to be recruited is fundamental for an adequate management of this resource for both fishing and aquaculture (MELO et al, 2013).…”

Reproductive cycle of the mangrove oyster, Crassostrea rhizophorae (Bivalvia: Ostreidae) cultured in a macrotidal high-salinity zone on the Amazon mangrove coast of Brazil

“…Oyster spats used in the culture were recruited using artificial PET bottle collectors installed in the Paciência estuary. Oyster spats were genetically ACTA AMAZONICA characterized by the PCR-Multiplex species-specific technique and confirmed as C. rhizophorae by the presence of 640 bp (Lopes et al 2018).…”

A Deficiência De Micronutrientes Em Crianças E Adolescentes Com Síndrome De Down

“…A utilização do gene Citocromo oxidase subunidade I permitiu inferir quanto a identificação do táxon analisado: A. flexuosa. Este marcador se mostrou como uma ferramenta poderosa em estudos filogenéticos, fornecendo informações sobre a sistemática do grupo moluscos bivalves (KAPPNER e BIELER, 2006;MIKELSEN et al, 2006;ARRUDA et al, 2009;TURECK, 2010;MELO et al, 2010;MELO et al, 2013;LOPES, et al, 2018;LOPES, et al, 2019).…”

“…Pesquisas genéticas de identificação e caracterização populacional, avaliação de estoque de moluscos bivalves vêm sendo realizados utilizando o gene Citocromo Oxidase subunidade I (COI) com a finalidade de auxiliar na identificação precisa das espécies (RODRIGUES et al, 2010;LEMER et al, 2014;FERNANDES et al, 2018;LOPES et al, 2018;LOPES, et al, 2019). Portanto, o trabalho teve por objetivo confirmar via DNA barcoding a sinonímia de Anomalocardia brasiliana com Anomalocardia flexuosa na Ilha de Upaon-Açu, Maranhão, Brasil.…”