PCR-based differentiation of Fusarium oxysporum ff. sp. lycopersici and radicis-lycopersici and races of F. oxysporum f. sp. lycopersici

Molecular fingerprinting of Fusarium oxysporum f. sp. passiflorae isolates using AFLP markers

“…However, the success in the differentiation of pathogenic isolates between different formae speciales has given rise to contradictions, for example, in not distinguishing isolates of F. solani that are pathogenic to either soybeans or beans from those that are pathogenic to both crops with the use of the ARDRA technique (Amplified Ribosomal DNA Restriction Analysis) (Oliveira and Costa, 2002). On the other hand, Werner and Irzykowska (2007) demonstrated the applicability of RAPD in differentiating between pathogenic and non-pathogenic isolates of F. oxysporum, and Hirano and Arie (2006) used molecular differences in the endo-polygalacturonase (pg1) and exo-polygalacturonase (pgx4) genes of F. oxysporum f. sp. lycopersici and radicis-lycopersici isolates to design a set of primers able to differentiate between the pathogenic types of F. oxysporum in tomato.…”

PCR detection of Fusarium oxysporum f.sp. radicis-lycopersici and races of F. oxysporum f.sp. lycopersici of tomato in protected tomato growing areas of Eastern Mediterranean Region of Turkey

“…The effectiveness of DNA isolation was checked in 0.8% agarose gel electrophoresis. To determine the formae speciales of FOL (race 1, 2, or 3) or FORL, PCR was performed with the specific primer sets uni, sp13, sp23, and sprl as described by Hirano and Arie (2006). Primers were synthesized by İontek (Adana, Turkey) ( Table 1).…”

“…A GeneAmp 9700 thermocycler (Eppendorf Mastercycler Gradient PCR Thermocycler) was used for PCR amplifications. The PCR conditions for all primers were set at the initial denaturation temperature of 94 °C for 5 min, followed by 45 cycles of 94 °C for 1 min, annealing at 61 °C for 1 min, and elongation at 72 °C for 2 min, with a final extension at 72 °C for 10 min (Hirano and Arie 2006). All PCR reaction products were electrophoresed in a 2% agarose gel, stained with ethidium bromide, and visualized under UV light.…”

Extractos de Larrea tridentata como una estrategia ecológica contra Fusarium oxysporum radicis-lycopersici en plantas de tomate bajo condiciones de invernadero

“…The fungus was distinguished by comparing its colonial and morphological characteristics using specialized literature (Leslie and Summerell, 2016;Nelson et al, 1983), which was identified molecularly with specific primers according to Hirano and Arie (2006) Preparation of the extracts. Extracts were produced using four solvents: dichloromethane (DCM), methanol (MeOH), ethanol (EtOH), and water (H 2 O) using young L. tridentata leaf and stem samples from the Sonora desert (Peñuelas et al, 2015).…”