PCR-Based Detection of Extended-Spectrum β-Lactamases (bla CTX-M-1 and bla TEM ) in Escherichia coli, Salmonella spp. and Klebsiella pneumoniae Isolated from Pigs in North Eastern India (Mizoram)

Lalzampuia, H.; Dutta, Tapan Kumar; Warjri, Iadarilin; Chandra, Rajesh

doi:10.1007/s12088-013-0378-z

Cited by 28 publications

(22 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some combinations of β-lactam resistance encoding genes were detected significantly. In contrast to our results, Lalzampuia et al, [43] found that for eight isolates, 7 harbored CTX-M-1 gene and/or TEM gene. The CTX-M and TEM were the most common gene combinations (33.33%) in E. coli isolates from broiler farms in the Philippines [44].…”

Section: Plos Onecontrasting

confidence: 99%

Characterization of β-lactam resistance in K. pneumoniae associated with ready-to-eat processed meat in Egypt

Abdel-Rhman

2020

PLoS ONE

View full text Add to dashboard Cite

K. pneumoniae was known as a nosocomial infection that causes human diseases. It is considered as one of the food-borne pathogens as it causes septicemia and diarrhea in humans. This study aims to characterize K. pneumoniae strains isolated from ready to eat processed meat phenotypically and genetically. Three hundred and fifty ready to eat processed meat (Luncheon-meat) samples were collected. Forty-four (12.6%) K. pneumoniae strains were isolated and bio-typed, where the majority were identified to belong to biotype B1. K1 and K2 serotypes were detected and strains were classified as hypermucoviscous K. pneumoniae (HVKP) and classic K. pneumoniae (CKP) (26 and 18 isolates, respectively). The isolates were resistant to several classes of β-lactam antibiotics, ceftazidim and cefotaxime (95.5%), cefoxitin (93.2%), ertapenem (90.9%) and amoxicillin-clavulanic acid (86.4%). They were classified as extended spectrum β-lactamases (ESBLs), AmpC or carbapenemase-producers phenotypically. Eighteen β-lactamase genes were investigated by PCR. The most prominent genes were SHV (63.6%), TEM (52.2%), CTX-M15 (50%), AMPC (47.7%), CIT-M (45.5%) and VIM (43.2%). Co-detection of β-lactam resistance genes revealed 42 gene profiles. Twenty-four isolates had the complete efflux system (AcrAB-ToƖC). Besides, Integrons (I, II, III) were detected in 20 isolates. Molecular typing by ERIC-PCR showed high genetic diversity between isolates as 34 different patterns were identified. Overall, this study confirmed the hazards posed by the presence of multiple resistance genes in the same isolate and this should not be undervalued. Besides, the horizontal transfer of plasmid harboring resistance genes between isolates in food represents potential health risks for consumers in Egypt and so the control and inhibition plans are necessary.

show abstract

Section: Plos Onecontrasting

confidence: 99%

Characterization of β-lactam resistance in K. pneumoniae associated with ready-to-eat processed meat in Egypt

Abdel-Rhman

2020

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Detection of bla CTX-M-1 and bla TEM in K . pneumoniae isolated from pigs in north eastern India has been reported previously [25]. In a previous study, Roy et al has demonstrated the high prevalence of bla TEM , bla SHV, bla CTX-M and bla QNR genes in pathogenic K .…”

Section: Introductionmentioning

confidence: 81%

Occurrence of Diverse Antimicrobial Resistance Determinants in Genetically Unrelated Biocide Tolerant Klebsiella pneumoniae

et al. 2016

View full text Add to dashboard Cite

Nosocomial infections due to Klebsiella pneumoniae is a significant problem in health care settings worldwide. In this study, we examined the antimicrobial susceptibility, genetic profiles and mechanisms of antibiotic resistance in K. pneumoniae isolates of Indian origin. To our knowledge this is the first report demonstrating the high prevalence of β-lactamases, aminoglycoside modifying enzymes, quinolone resistance genes besides demonstrating the involvement of active efflux in K. pneumoniae Indian isolates. This study has enabled us to correlate the phenotypic and genotypic characteristics in K. pneumoniae, providing an important base for continued monitoring and epidemiological studies of this emerging nosocomial pathogen in Indian hospitals.

show abstract

“…Lalzampuia et al . [ 11 ] reported from the same laboratory that none of the 102 E. coli isolates from pigs in Mizoram were carrying bla TEM gene. The bla TEM genes also have a tendency to mutate and secrete enzymes with extended spectrum of activity, which could have accounted for the high resistance to ampicillin in the population studied [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

Extended-spectrum β-lactamases producing multidrug resistance Escherichia coli, Salmonella and Klebsiella pneumoniae in pig population of Assam and Meghalaya, India

Lalruatdiki¹,

Dutta²,

Roychoudhury³

et al. 2018

Vet World

Self Cite

View full text Add to dashboard Cite

Aim:The present study was conducted to record the prevalence of extended spectrum β-lactamases (ESBLs) producing Escherichia coli, Salmonella spp., and Klebsiella pneumoniae from pig population of Assam and Meghalaya and to record the ability of the resistant bacteria to transfer the resistance genes horizontally.Materials and Methods:Fecal samples (n=228), collected from pigs of Assam (n=99) and Meghalaya (n=129), were processed for isolation and identification of E. coli and Salmonella spp. All the isolates were tested for ESBLs production by double disc synergy test (DDST) followed by screening for ESBLs producing genes (blaTEM, blaSHV, blaCTX-M, and blaCMY) by polymerase chain reaction (PCR). Possible transfer of resistance encoding genes between enteric bacterial species was carried out by in vitro and in vivo horizontal gene transfer (HGT) method.Results:A total of 897 enteric bacteria (867 E. coli and 30 Salmonella) were isolated and identified. Altogether 25.41% isolates were confirmed as ESBL producers by DDST method. Majority of the isolates were E. coli followed by Salmonella. By PCR, 9.03% isolates were found positive for at least one of the target resistance genes. blaSHV was absent in all the isolates. blaCMY was the most prevalent gene. All the E. coli isolates from Assam were negative for blaTEM. A total of 2.76% isolates were positive for blaTEM + blaCMY. On the other hand, 0.67% isolates were positive for blaCTX-M + blaCMY genes. Only 0.33% isolates carried all the three genes. Altogether, 4.68% bacteria carried the resistance encoding genes in their plasmids. blaTEM gene could be successfully transferred from Salmonella (donor) to E. coli (recipient) by in vitro (5.5-5.7×10−5) and in vivo (6.5×10−5 to 8.8×10−4) methods. In vivo method was more effective than in vitro in the transfer of resistance genes.Conclusion:The pig population of Assam and Meghalaya are carrying multidrug resistance and ESBLs producing E. coli and Salmonella. The isolates are also capable to transfer their resistance trait to other bacterial species by HGT. The present finding could be considered as a serious public health concern as similar trait can also be transmitted to the human commensal bacteria as well as pathogens.

show abstract

PCR-Based Detection of Extended-Spectrum β-Lactamases (bla CTX-M-1 and bla TEM ) in Escherichia coli, Salmonella spp. and Klebsiella pneumoniae Isolated from Pigs in North Eastern India (Mizoram)

Cited by 28 publications

References 32 publications

Characterization of β-lactam resistance in K. pneumoniae associated with ready-to-eat processed meat in Egypt

Characterization of β-lactam resistance in K. pneumoniae associated with ready-to-eat processed meat in Egypt

Occurrence of Diverse Antimicrobial Resistance Determinants in Genetically Unrelated Biocide Tolerant Klebsiella pneumoniae

Extended-spectrum β-lactamases producing multidrug resistance Escherichia coli, Salmonella and Klebsiella pneumoniae in pig population of Assam and Meghalaya, India

Contact Info

Product

Resources

About