Occurrence of Diverse Antimicrobial Resistance Determinants in Genetically Unrelated Biocide Tolerant Klebsiella pneumoniae

Mondal, Amitabha; Venkataramaiah, Manjunath; Rajamohan, Govindan; Srinivasan, Vijaya Bharathi

doi:10.1371/journal.pone.0166730

Cited by 6 publications

(1 citation statement)

References 40 publications

(52 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, 66%, 11% and 13% of the isolates had blaSHV, rmtB, and rmtC, respectively. This is consistent with our results, showing the highest resistance to kanamycin; however, the range of bla SHV and rmtB was reported to be higher than that in our study (35).…”

Section: Discussionsupporting

confidence: 93%

Assessment of 16srRNA Methylase Genes Among Non-ESBL and ESBL-Producing Klebsiella pneumoniae Isolates

Pakzad

Samadi

Imanieini

et al. 2020

Arch Clin Infect Dis

View full text Add to dashboard Cite

The aim of our study was to investigate mechanisms of aminoglycoside resistance in extended-spectrum beta-lactamases (ESBL)producing Klebsiella pneumoniae (K. pneumoniae) isolates from Iran. To this end, 154 clinical isolates of K. pneumoniae were collected from two hospitals in Ilam city, Iran. The Kirby-Bauer (agar diffusion) antibiotic testing method was used to determine the susceptibility pattern of the isolates against kanamycin, gentamicin, tobramycin, netilmicin and amikacin. Aminoglycoside acetyltransferases (aac(3)-IIa, aac(6')-Ib, and aac(3)-Ia), 16SrRNA methylase genes (armA and rmtB) and ESBL genes (blaTEM, blaSHV , and blaCTX-M) were detected by PCR amplification. 59.1% (n = 91) of K. pneumoniae isolates were detected ESBL producers with the phenotypic test. Moreover, blaTEM, blaSHV and blaCTX-M were detected in 83.5% (n=76), 52.7% (n=48) and 26.4% (n=24) of the ESBL-producing isolates, respectively. Among 52 resistant or intermediate isolates against aminoglycosides, the aac(3)-IIa, aac(6')-Ib and rmtB genes were detected in 55.8% (n = 29), 80.8% (n = 42) and 1.9% (n = 1) of the isolates, respectively; none of the isolates, however, had the aac(3)-Ia and armA genes. Therefore, the results showed the high prevalence of aminoglycosides resistance in the K. pneumoniae isolates. As observed, the acetyltransferase modifying enzymes (aac genes) played major roles in determining this resistance. However, the rate of 16srRNA methylase genes was extremely low in K. pneumoniae.

show abstract

Section: Discussionsupporting

confidence: 93%