PCR-based Characterization of Mung Bean (Vigna radiata) Genotypes from Indian Subcontinent at Intra- and Inter-Specific Level

Chattopadhyay, Krishnendu; Bhattacharya, Sandip; Mandal, Nirmal; Sarkar, H. K.

doi:10.1007/bf03263275

Cited by 12 publications

(8 citation statements)

References 17 publications

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“…Genetic integrity of the in vitrogenerated and acclimatized propagules was assessed through DNA fingerprinting with 10 ISSR primers ; Table 5). Initially, the genomic DNA was extracted from 80 mg tender leaves according to the procedure described by Chattopadhyay et al (2008). The extracted DNA samples were then subjected to the polymerase chain reaction (PCR) amplification using 10 ISSR primers as mentioned above.…”

Section: Acclimatizationmentioning

confidence: 99%

An elite protocol for accelerated quality-cloning in Gerbera jamesonii Bolus cv. Sciella

Gantait

Mandal

Bhattacharyya

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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A novel, efficient, and simple protocol was developed on in vitro mass propagation and acclimatization of Gerbera jamesonii Bolus cv. Sciella, an ornamental plant with attractive flowers. Shoot tip was used as the primary explant for in vitro establishment in which Murashige and Skoog (MS) medium supplemented with a low level of NAA (0.5 mgl −1 ) and BAP (1.5 mgl −1 ) promoted earliest axillary bud initiation within 5 d in 91.6% of the inoculants. Five axillary buds were initiated from a single explant within 13 d after inoculation. A very high rate of shoot multiplication (14 shoots per inoculated axillary bud) and proliferation was achieved when MS medium was fortified with a relatively higher level of BAP (2 mgl −1 ) and 60 mgl −1 ADS within 27 d of multiple shoot culture. A maximum number of well-developed roots per plant was observed in MS medium with 0.5 mg l −1 IAA in the next 26 d. In the easy low-cost acclimatization process of 20 d, a combination of sand, soil, cow urine, and tea leaves extract (1:1:1:1; v/v) ensured 95% survival rate. Sixty-one well-acclimatized plants were obtained from a single shoot tip within 86 d. The sustained multiple shoot culture for 15 mo paved the way toward the conservation of genetic resources as well as beneficial economics. The clonal fidelity study of micropropagated and sustained cultured clones using ISSR primers ensured the continuous supply of quality propagules retaining genetic uniformity. The in vitro-generated plants performed better over conventionally propagated plants in the field condition.

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Section: Acclimatizationmentioning

confidence: 99%

An elite protocol for accelerated quality-cloning in Gerbera jamesonii Bolus cv. Sciella

Gantait

Mandal

Bhattacharyya

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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“…A few Sublobata lines including Sub2 having different stress tolerance (Dana 1980(Dana , 1976 was used as a source of Bruchid resistance locus. Description of all these genotypes is as that of Chattopadhyay et al 2008. Fig.…”

Section: Plant Materialsmentioning

confidence: 99%

“…PCR from greengram genomic DNA Genomic DNA was extracted from leaves of two parents and 113 selected F 2 segregants following method as described earlier (Chattopadhyay et al 2008). Twelve pairs of SSR primers of V. radiata (Kumar et al 2002); thirty eight pairs of SSR (Simple sequence repeat) primers of V. angularis (Willd.)…”

Section: Plant Materialsmentioning

confidence: 99%

Molecular markers linked with bruchid resistance in Vigna radiata var. Sublobata and their validation

Sarkar

Ghosh

Chatterjee

et al. 2011

J. Plant Biochem. Biotechnol.

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“…Genomic DNA was extracted from 60 mg tender leaves according to the procedure described by Chattopadhyay et al (2008). The 25 ll optimized polymerase chain reaction (PCR) mixture contained 50 ng DNA, 2.5 ll 10 9 Taq polymerase assay buffer, 4.0 ll 2.5 mM dNTPs, 0.5 U Taq DNA polymerase (Sigma) and 200 ng of primer (Sigma).…”

Section: Clonal Fidelity Assessmentmentioning

confidence: 99%

Direct induction of protocorm-like bodies from shoot tips, plantlet formation, and clonal fidelity analysis in Anthurium andreanum cv. CanCan

et al. 2012

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Protocorm-like bodies (PLBs) were induced directly at high frequency from wounded surface of Anthurium andreanum cv. CanCan shoot tip-ends, used as explants. In order to obtain PLB directly, the influence of different types and concentrations of cytokinins were evaluated. Amid the cytokinins, N 6 -(D 2 -isopentenyl)-adenine (2-iP) at a concentration of 15 lM was most effective in inducing PLB whereby *98 (97.8) % of explants induced PLB with an average of 120 PLBs per shoot tip within 50 days of culture. Stereomicroscopic observation meticulously revealed the sequential changes from initiation to maturation of PLB gradually forming shoot apical meristem, shoot primordia and leaf primordia. Mature PLBs showed significant shoot proliferation (98.4 %) in media containing 10 lM 6-furfurylaminopurine forming 17 shoots per PLB within 30 days. The inclusion of activated charcoal (AC) in media containing auxin had promotive effect on rooting whereby 5 lM indole-3-butyric acid plus 500 lM AC resulted in highest number and length of roots. Successfully acclimatized plants, subjected to random amplified polymorphic DNA assessment for genetic fidelity, did not show any variation. Thus, this complete study has successfully outlined a rapid, high frequency direct induction of PLB of Anthurium from shoot tips inclusive of shoot proliferation, rooting and acclimatization.

show abstract

PCR-based Characterization of Mung Bean (Vigna radiata) Genotypes from Indian Subcontinent at Intra- and Inter-Specific Level

Cited by 12 publications

References 17 publications

An elite protocol for accelerated quality-cloning in Gerbera jamesonii Bolus cv. Sciella

An elite protocol for accelerated quality-cloning in Gerbera jamesonii Bolus cv. Sciella

Molecular markers linked with bruchid resistance in Vigna radiata var. Sublobata and their validation

Direct induction of protocorm-like bodies from shoot tips, plantlet formation, and clonal fidelity analysis in Anthurium andreanum cv. CanCan

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