Molecular markers linked with bruchid resistance in Vigna radiata var. Sublobata and their validation

Sarkar, S.; Ghosh, Sandipan; Chatterjee, Moulita; Das, P. K.; Lahari, Triparna; Maji, Anirban; Mondal, N; Pradhan, K.; Bhattacharyya, Sukanta

doi:10.1007/s13562-011-0039-4

Cited by 22 publications

(13 citation statements)

References 14 publications

(16 reference statements)

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“…The authors reported close linkage in a recombinant inbred line (RIL) population between ACC41 and ‘Berken’. Further, Sarkar et al (2011) showed that STSbr1 amplified a 225bp fragment in V. sublobata accession (sub2) and 12 other cultivars that were resistant to bruchids. Though RAPD markers are fast and simple, the distance between them is high from the bruchids resistant gene.…”

Section: Sources Of Resistance Against Insect-pestsmentioning

confidence: 99%

Biotic and Abiotic Constraints in Mungbean Production—Progress in Genetic Improvement

Nair¹,

Pandey²,

War³

et al. 2019

Front. Plant Sci.

163

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Mungbean [Vigna radiata (L.) R. Wilczek var. radiata] is an important food and cash legume crop in Asia. Development of short duration varieties has paved the way for the expansion of mungbean into other regions such as Sub-Saharan Africa and South America. Mungbean productivity is constrained by biotic and abiotic factors. Bruchids, whitefly, thrips, stem fly, aphids, and pod borers are the major insect-pests. The major diseases of mungbean are yellow mosaic, anthracnose, powdery mildew, Cercospora leaf spot, halo blight, bacterial leaf spot, and tan spot. Key abiotic stresses affecting mungbean production are drought, waterlogging, salinity, and heat stress. Mungbean breeding has been critical in developing varieties with resistance to biotic and abiotic factors, but there are many constraints still to address that include the precise and accurate identification of resistance source(s) for some of the traits and the traits conferred by multi genes. Latest technologies in phenotyping, genomics, proteomics, and metabolomics could be of great help to understand insect/pathogen-plant, plant-environment interactions and the key components responsible for resistance to biotic and abiotic stresses. This review discusses current biotic and abiotic constraints in mungbean production and the challenges in genetic improvement.

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Section: Sources Of Resistance Against Insect-pestsmentioning

confidence: 99%

Biotic and Abiotic Constraints in Mungbean Production—Progress in Genetic Improvement

Nair¹,

Pandey²,

War³

et al. 2019

Front. Plant Sci.

163

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“…Great efforts have been invested in mapping a Br gene in mungbean [ 19 ]. First, a single Br gene was proposed in TC1966, and later additional minor modifier factors were postulated [ 16 , 17 , 44 ]; nevertheless, the nature of the Br gene(s) of TC1966 remained unclear. Biochemically, several Br candidate genes highly associated with bruchid resistance have been reported, including the Va gene for vignatic acid biosynthesis [ 20 ]; vicilins, also known as 7S storage globulins [ 22 ]; and the cysteine-rich protein VrD1 [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

Genomic and transcriptomic comparison of nucleotide variations for insights into bruchid resistance of mungbean (Vigna radiata [L.] R. Wilczek)

Liu

Kuo

et al. 2016

BMC Plant Biol

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BackgroundMungbean (Vigna radiata [L.] R. Wilczek) is an important legume crop with high nutritional value in South and Southeast Asia. The crop plant is susceptible to a storage pest caused by bruchids (Callosobruchus spp.). Some wild and cultivated mungbean accessions show resistance to bruchids. Genomic and transcriptomic comparison of bruchid-resistant and -susceptible mungbean could reveal bruchid-resistant genes (Br) for this pest and give insights into the bruchid resistance of mungbean.ResultsFlow cytometry showed that the genome size varied by 61 Mb (mega base pairs) among the tested mungbean accessions. Next generation sequencing followed by de novo assembly of the genome of the bruchid-resistant recombinant inbred line 59 (RIL59) revealed more than 42,000 genes. Transcriptomic comparison of bruchid-resistant and -susceptible parental lines and their offspring identified 91 differentially expressed genes (DEGs) classified into 17 major and 74 minor bruchid-resistance–associated genes. We found 408 nucleotide variations (NVs) between bruchid-resistant and -susceptible lines in regions spanning 2 kb (kilo base pairs) of the promoters of 68 DEGs. Furthermore, 282 NVs were identified on exons of 148 sequence-changed-protein genes (SCPs). DEGs and SCPs comprised genes involved in resistant-related, transposable elements (TEs) and conserved metabolic pathways. A large number of these genes were mapped to a region on chromosome 5. Molecular markers designed for variants of putative bruchid-resistance–associated genes were highly diagnostic for the bruchid-resistant genotype.ConclusionsIn addition to identifying bruchid-resistance-associated genes, we found that conserved metabolism and TEs may be modifier factors for bruchid resistance of mungbean. The genome sequence of a bruchid-resistant inbred line, candidate genes and sequence variations in promoter regions and exons putatively conditioning resistance as well as markers detecting these variants could be used for development of bruchid-resistant mungbean varieties.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0736-1) contains supplementary material, which is available to authorized users.

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“…sublobata accession ( Sub2 ) and also in some of the mung bean cultivars with contrasting host response to bruchid infestation. It behaves as a dominant marker among the Indian genotypes, and is efficiently used for screening bruchid‐resistant genotypes (Sarkar et al ., ). Blair et al .…”

Section: Biotechnological Approachesmentioning

confidence: 97%

“…Only in mung bean, a STS marker ( STSbr1 ) which was reported to be associated with the bruchid resistance gene in the Australian V. radiata var. sublobata ( ACC41 ), was validated among the Indian sublobata accession ( Sub2 ) and mung bean cultivars conferring bruchid resistance (Sarkar et al ., ). The utilisation of this marker in the MAB (Man and the Biosphere) programmes of mung bean is under progress.…”

Section: Biotechnological Approachesmentioning

confidence: 97%

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Bruchid pest management in pulses: past practices, present status and use of modern breeding tools for development of resistant varieties

Mishra

Macedo

Panda

et al. 2017

Annals of Applied Biology

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Bruchids (Callosobruchus spp.) are recognised as the most detrimental storage pest of pulses, especially in the tropics and subtropics. They invade matured pods as well as seeds during storage and, to some extent, farming fields, in turn reducing the net yield of the crops. Several approaches including cultural, biological, physical and chemical control measures have been implemented with the aim of managing these pests, but none of these have been successful across time and space. Recently, transgenic-and marker-assisted breeding approaches have appeared as promising tools for the successful management of these pests. Although some efforts have been made on the development of bruchid-resistant transgenic crops, the cultivars developed are yet to be commercialised worldwide because of various limitations. In contrast, marker-assisted breeding involving the identification of DNA-based markers linked to host resistance against bruchids, have shown some success in the quest for the development of bruchid-resistant cultivar(s). DNA markers linked to bruchid resistance have been identified in various grain legumes, particularly in the genus Vigna, and include mung bean (Vigna radiata), azuki bean (Vigna angularis), rice bean (Vigna umbellata), cowpea (Vigna unguiculata) and black gram (Vigna mungo). After their validation in different genetic backgrounds, these markers could be utilised for marker-assisted selection and breeding ventures to protect pulse crops. The present study discusses the pros and cons of different approaches for the successful management of the bruchid pests in pulses. The review also highlights about the integrative approach aided with molecular interventions to improve productivity by avoiding losses incurred due to bruchids, and to attain sustainable yields for major pulse crops.

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Molecular markers linked with bruchid resistance in Vigna radiata var. Sublobata and their validation

Abstract: Bruchid, Callosobruchus chinensis (L.) is an important pest of Vigna radiata during storage. RFLP and PCR based markers identified, linked with bruchid resistance gene in wild accession of greengram (V. radiata var.

Cited by 22 publications

References 14 publications

Biotic and Abiotic Constraints in Mungbean Production—Progress in Genetic Improvement

Biotic and Abiotic Constraints in Mungbean Production—Progress in Genetic Improvement

Genomic and transcriptomic comparison of nucleotide variations for insights into bruchid resistance of mungbean (Vigna radiata [L.] R. Wilczek)

Bruchid pest management in pulses: past practices, present status and use of modern breeding tools for development of resistant varieties

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