PCR Amplification from Fixed Tissue Indicates Frequent Involvement of
            <i>Brachyspira aalborgi</i>
            in Human Intestinal Spirochetosis

Mikosza, Andrew S. J.; La, Tom; Brooke, C.J.; Lindboe, Christian F.; Ward, Peter; Heine, Ralf G.; Guccion, John G.; Boer, W. Bastiaan de; Hampson, D.J.

doi:10.1128/jcm.37.6.2093-2098.1999

Cited by 70 publications

(47 citation statements)

References 42 publications

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“…Studies using enzymatic and genetic analysis have shown that there are several species of intestinal spirochetosis. Mikosza et al [6] reported the organism to be related to Brachyspira aalborgia, which is probably non pathogenic in the immunocompetent patient, however, a study to Trivett-Moore et al [7] suggested Serpulina pilosicoli to be the agent in patients with symptomatic spirochetosis. The latter organism showed attachment of a phenotypically and genotypically distinct weakly beta haemolytic intestinal spirochete to the brush border of colonic enterocytes suggesting enteropathogenecity [10] and the same organism has been shown to be enterotoxic in animal models resulting in watery diarrhoea [11,12].…”

Section: Discussionmentioning

confidence: 99%

“…While some authors suggest that the organism is non pathogenic, others have shown clinical manifestations of symptoms such as diarrhoea and a clinical response to antimicrobial therapy [4,5]. No common organism has been cultured in a large number of cases of spirochetosis, but a Brachyspira aalborgi specific sequence was detected by PCR in 62.5% of patients with spirochetosis [6]. In another study Serpulina pilosicoli was recovered from rectal biopsies in 50% of symptomatic patients with a histological diagnosis of intestinal spirochetosis [7].…”

Section: Introductionmentioning

confidence: 99%

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Intestinal spirochetosis: clinicopathological features with review of the literature

Alsaigh

Fogt

2002

Colorectal Disease

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OBJECTIVE: The purpose of this study is to evaluate the clinical presentation of patients with intestinal spirochetosis, as it is has not been well described in the literature. METHODS: We studied 15 patients with colonic biopsies that showed spirochetosis. The study group consisted of 11 males and 4 females, 10 of the males were heterosexual and one was homosexual. None of these patients were clinically immunocompromized. A colonoscopy was performed for evaluation of different symptoms. The histological diagnosis of spirochetosis was made on H&E examination and confirmed by Giemsa and/or Steiner stain. There was no evidence of associated active or any specific colitis in any of the cases. The control group consisted of 30 patients, 16 males and 14 females that were matched for age and clinical indications for obtaining a colonic biopsy. RESULTS: Colonic mucosa with prominent brush border-like surface colonized with large amounts of spirochetes was noted in all 15 cases in the study group and in none of 30 cases in the control group. The clinical presentation in patients with spirochetosis was compared to the control group. Of the spirochetosis patients 86% presented with some form of clinical symptoms compared with 13% of controls. These symptoms included chronic watery diarrhoea in 40% spirochetosis patients vs 5% in controls; a change in bowel habit was present in 33% of spirochetosis patients vs 3.3% in controls. The endoscopic appearance in spirochetosis patients was reported as normal in six patients, 'polyoid' in seven patients, erythematous in one patient and 'lesion' in one patient. CONCLUSION: These findings suggest that colonic spirochetosis affects a heterogenous group of patients, the majority of whom presented with gastrointestinal symptoms. The variable clinical findings may be related to the infecting organism and the condition may not be harmless in all patients.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Intestinal spirochetosis: clinicopathological features with review of the literature

Alsaigh

Fogt

2002

Colorectal Disease

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“…A 471 base pair sequence of the 16S rRNA gene of B. aalborgi, equivalent to the base pair positions 172^675 of the 16S rRNA gene of Escherichia coli, was targeted for PCR ampli¢cation, as described previously [4], except that dimethyl sulfoxide was not added. Furthermore, the 16S rRNA gene of B. pilosicoli was targeted for PCR ampli¢cation as described previously [4], except that a di¡erent reverse primer (5P-CCCCTACAATATCCAAGACT-3P) was used. This produced a 439 bp product equivalent to the base pair positions 204^676 of the 16S rRNA gene of E. coli [16].…”

Section: Polymerase Chain Reactionsmentioning

confidence: 99%

“…Two species of anaerobic intestinal spirochaetes, Brachyspira (formerly Serpulina) pilosicoli [1] and Brachyspira aalborgi [2], colonise the human large intestine [3,4]. Both species are associated with a condition known as intestinal spirochaetosis (IS), in which a thin carpet-like layer of spirochaetes is found attached by one cell end to the colorectal surface epithelium [5].…”

Section: Introductionmentioning

confidence: 99%

“…Speci¢c information about the prevalence of B. aalborgi came from studies using the polymerase chain reaction (PCR) on DNA extracted from colorectal biopsy samples. It was the most common of the two spirochaete species involved in IS in humans in Norway, the USA and Australia [4,16]. Currently there is a lack of information about the distribution of B. aalborgi in human populations, directly related to the absence of reliable means to culture the spirochaete, and a lack of PCR or similar methods to detect the spirochaete in faeces.…”

Section: Introductionmentioning

confidence: 99%

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PCR detection of Brachyspira aalborgi and Brachyspira pilosicoli in human faeces

Mikosza¹

2001

FEMS Microbiology Letters

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Previously-developed PCR protocols specific for the 16S rRNA gene of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli were adapted for the detection of these species in human faeces, following DNA extraction and purification using mini-prep columns. The limits of detection in seeded faeces for B. aalborgi and B. pilosicoli respectively were 2U10 2 and 7U10 3 cells per PCR reaction, equivalent to 5U10 4 and 1U10 5 cells per g of faeces. The PCR techniques were applied to faecal samples from two patients with histological evidence of intestinal spirochaetosis. In the first patient, in whom B. aalborgi had been identified by 16S rDNA PCR from colonic biopsies, a positive amplification for B. aalborgi only was obtained from the faeces. The organism could not be isolated from these faeces. In the second patient, both colonic biopsies and faeces were PCR positive for B. pilosicoli only, and B. pilosicoli was isolated from the faeces. These new faecal PCR protocols should be valuable for future studies on the epidemiology of intestinal spirochaete infections in human populations, particularly as it is not currently possible to isolate B. aalborgi from faeces. ß

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The Genus Brachyspira

Stanton¹

2006

The Prokaryotes

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PCR Amplification from Fixed Tissue Indicates Frequent Involvement of Brachyspira aalborgi in Human Intestinal Spirochetosis

Cited by 70 publications

References 42 publications

Intestinal spirochetosis: clinicopathological features with review of the literature

Intestinal spirochetosis: clinicopathological features with review of the literature

PCR detection of Brachyspira aalborgi and Brachyspira pilosicoli in human faeces

The Genus Brachyspira

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