PCR detection of Brachyspira aalborgi and Brachyspira pilosicoli in human faeces

Mikosza, A

doi:10.1016/s0378-1097(01)00100-8

Cited by 18 publications

(27 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The sensitivity of PCR for detection of B. hyodysenteriae was higher than that for detection of B. pilosicoli and similar to the sensitivity of culture. The sensitivities of PCRs targeting the 23S rRNA genes or other genes of Brachyspira species for various species of intestinal spirochaetes have been reported (Park et al, 1995;Leser et al, 1997;Atyeo et al, 1998;Barcellos et al, 2000;Mikosza et al, 2001). These PCRs were applied directly to spirochaete cells or to spirochaetal growth on primary isolation plates, and their sensitivities were generally consistent with those of the PCR used in the present study.…”

Section: Resultssupporting

confidence: 82%

Immunomagnetic separation of the intestinal spirochaetes Brachyspira pilosicoli and Brachyspira hyodysenteriae from porcine faeces

Corona-Barrera¹,

Smith²,

La³

et al. 2004

Journal of Medical Microbiology

View full text Add to dashboard Cite

Porcine intestinal spirochaetes are fastidious anaerobic organisms and, as a consequence, it has been necessary to develop various protocols to enhance their isolation from or detection in faeces.Immunomagnetic separation (IMS) is a method developed recently to improve separation of target cells from mixed cell suspensions. The purpose of the present study was to compare the relative sensitivity of IMS for isolation of Brachyspira pilosicoli and Brachyspira hyodysenteriae with current routine diagnostic methods (culture on selective media and PCR) for detection of these microorganisms in pig faeces. Neither direct nor indirect IMS methods enhanced the sensitivity of detection of either organism when performed with the recommended washings during sample processing. Performance of the IMS procedure without washing gave sensitivity at levels similar to direct culture onto selective medium. Further development of IMS techniques is required to improve isolation rates of Brachyspira species from faecal samples. INTRODUCTIONIntestinal spirochaetes are mainly members of the genus Brachyspira, which includes species commensal and pathogenic for pigs and other animals and humans. Brachyspira hyodysenteriae is recognized as the aetiological agent of swine dysentery (SD) (Taylor & Alexander, 1971) and Brachyspira pilosicoli is the aetiological agent of a disease of pigs known as intestinal spirochaetosis (Taylor et al., 1980) or porcine colonic spirochaetosis (PCS;Duhamel et al., 1998). Infection with B. pilosicoli has been reported in a wide range of hosts, including dogs (Duhamel et al., 1995), chickens and other avian species (Dwars et al., 1992;McLaren et al., 1997), guinea pigs (Vanrobaeys et al., 1998), non-human primates (Takeuchi et al., 1974) and humans (Cooper et al., 1986; Surawicz et al., 1987). As interest in the field of intestinal spirochaetes has grown, further species have been identified recently as pathogens in animal species other than pigs. For instance, Brachyspira alvinipulli has been described and shown to be pathogenic for chickens (Stanton et al., 1998) and the provisionally designated 'Serpulina canis' has been found in dogs (Duhamel et al., 1998).Porcine intestinal spirochaetes are Gram-negative, anaerobic and fastidious organisms. As a result, various culture media and incubation conditions have been assessed in order to develop the most reliable and efficient method for their isolation from faecal samples. Typically, isolation of these bacteria is achieved by culture on selective media based on trypticase soy agar or Columbia agar base supplemented with blood and multiple antibiotics to reduce the growth of other bacteria. Various antibiotic combinations have been utilized including spectinomycin (TSA-S400 medium; Songer et al., 1976), spiramycin-colistin-vancomycin (TSA-CVS medium;Jenkinson & Wingar, 1981) and spectinomycin-colistinvancomycin-spiramycin-rifampicin (BJ medium; Kunkle & Kinyon, 1988). In a comparative study of these media, BJ proved to be the most efficient in eliminating norm...

show abstract

Section: Resultssupporting

confidence: 82%

Immunomagnetic separation of the intestinal spirochaetes Brachyspira pilosicoli and Brachyspira hyodysenteriae from porcine faeces

Corona-Barrera¹,

Smith²,

La³

et al. 2004

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…The PCRs used for the detection of B. pilosicoli and B. aalborgi were the same as described previously for identification of the two organisms in human faeces [11,19], with the primers and PCR conditions being as described by Mikosza and colleagues [3].…”

Section: Pcr Assaysmentioning

confidence: 99%

Comparison of prevalence and risk factors for faecal carriage of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli in four Australian populations

2005

View full text Add to dashboard Cite

SUMMARYThis study examined the prevalence of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli in different Western Australian (WA) populations. Faecal samples included 287 from rural patients with gastrointestinal symptoms, comprising 142 from non-Aboriginal and 145 from Aboriginal people ; 227 from recent healthy migrants to WA from developing countries; and 90 from healthy non-Aboriginal individuals living in Perth, WA. DNA was extracted from faeces, and subjected to PCR assays for both species. B. pilosicoli-positive individuals were confined to the rural Aboriginal (14 . 5 %) and migrant (15 . 0%) groups. B. aalborgi was detected at a lower but similar prevalence in all four groups: rural non-Aboriginals, 5 . 6% ; rural Aboriginals, 6 . 9% ; migrants, 7 . 9% ; controls, 5 . 6 %. In migrants and Aborigines, the presence of B. pilosicoli and B. aalborgi was associated (P<0 . 001), suggesting that colonization by B. pilosicoli may be facilitated by colonization with B. aalborgi. Amongst the Aboriginal patients, logistic regression identified both spirochaete species as being associated with chronic diarrhoea, failure to thrive and being underweight. Both species may have pathogenic potential, but B. aalborgi appears more host-adapted than the opportunistic B. pilosicoli.

show abstract

“…The PCRs used were based on those described for detection of the spirochaete species in human colorectal biopsies and faeces [25,28,30]. For B. aalborgi, a 471-bp section of the 16S rRNA gene equivalent to the base-pair positions 172-675 of the 16S rRNA gene of Escherichia coli was targeted for PCR amplification.…”

Section: Pcrsmentioning

confidence: 99%

“…Although one histological study in Southern India demonstrated that IS occurs commonly in apparently healthy villagers [29], the spirochaetes present in these samples were not identified. Recently, the PCR techniques used on DNA extracted from biopsies have been adapted for the detection of B. aalborgi and B. pilosicoli in human faeces [30]. Faecal samples are much easier to obtain than are colorectal biopsies, and hence this technology will facilitate epidemiological studies on these spirochaetes.…”

Section: Introductionmentioning

confidence: 99%

Colonization and risk factors for Brachyspira aalborgi and Brachyspira pilosicoli in humans and dogs on tea estates in Assam, India

et al. 2004

View full text Add to dashboard Cite

SUMMARYThe prevalence of colonization with the anaerobic intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli was investigated in humans (n=316) and dogs (n=101) living on three tea estates in Assam, India. Colonization was detected using PCR on DNA from faeces. Nineteen (6 %) human faecal samples contained B. aalborgi DNA, 80 (25 . 3 %) contained B. pilosicoli DNA, and 10 (3 . 2%) contained DNA from both species. One canine sample contained DNA from B. pilosicoli. Significant factors for B. aalborgi colonization in logistic regression were : infection of family members with B. aalborgi (P<0 . 001), being a resident of Balipara (P=0 . 03), and use of water treatment (P=0 . 03). For B. pilosicoli, significant factors were : other family members being positive for B. pilosicoli (P<0 . 001), water obtained from a well (P=0 . 006), water treatment (P=0 . 03), and not having visited a doctor in the previous 12 months (P=0 . 03).

show abstract

PCR detection of Brachyspira aalborgi and Brachyspira pilosicoli in human faeces

Cited by 18 publications

References 18 publications

Immunomagnetic separation of the intestinal spirochaetes Brachyspira pilosicoli and Brachyspira hyodysenteriae from porcine faeces

Immunomagnetic separation of the intestinal spirochaetes Brachyspira pilosicoli and Brachyspira hyodysenteriae from porcine faeces

Comparison of prevalence and risk factors for faecal carriage of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli in four Australian populations

Colonization and risk factors for Brachyspira aalborgi and Brachyspira pilosicoli in humans and dogs on tea estates in Assam, India

Contact Info

Product

Resources

About