Patterns of organ development in plant tissue culture and the problem of organ determination

Hicks, G. S.

doi:10.1007/bf02860865

Cited by 78 publications

(36 citation statements)

References 59 publications

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“…Increased efficiency in terms of the percentage of regeneration and the number of produced shoot explants was observed on BM without growth regulators. This result can be explained with the presence of predetermined cells which first dedifferentiate themselves, subsequently form new meristematic centres, and finally differentiate again producing new organs [34][35][36][37][38][39]. This process, due to cell totipotency, has been observed in several species [30,[40][41][42][43][44][45][46][47] and is very efficient for regeneration.…”

Section: Discussionmentioning

confidence: 99%

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

Perrini

Alba

Ruta

et al. 2009

Cellular and Molecular Biology Letters

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A protocol for the induction of regeneration from leaves of Helichrysum italicum was established. Calli were found to form on the basal medium only when it was supplemented with thidiazuron (TDZ) alone or in combination with naphthalene acetic acid (NAA), with a percentage ranking of at least 80%. The hormone-free medium showed the highest percentage of shoot regeneration (62%) even though no callus formed. AFLP markers were employed to verify tissue culture-induced variation in the regenerated plantlets obtained by direct shoot regeneration or the indirect shoot regeneration process (callus formation). Seven out of the eleven AFLP primer pairs yielded polymorphic patterns. The average number of fragments per primer pair was 64.1. Singletons were represented by 12 (2.7%) fragments. Student’s T-test was performed both on the average number of shared fragments and on the nucleotide diversity, and no significant statistical difference was observed between the two regeneration treatments.

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Section: Discussionmentioning

confidence: 99%

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

Perrini

Alba

Ruta

et al. 2009

Cellular and Molecular Biology Letters

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“…What acquisition of competence is in cellular or molecular terms is not known. It is generally thought that preincubation on CIM is required to permit the dedifferentiation of tissues that will ultimately redifferentiate into organs (Gautheret, 1966;Hicks, 1980). When CIM-preincubated root explants are transferred to a cytokinin-rich SIM, they first become committed to form shoots (will form shoots if transferred to basal medium) and then shoots emerge (Cary et al, 2002).…”

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confidence: 99%

Gene Expression Programs during Shoot, Root, and Callus Development in Arabidopsis Tissue Culture

et al. 2006

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Shoots can be regenerated from Arabidopsis (Arabidopsisthaliana) root explants in tissue culture through a two-step process requiring preincubation on an auxin-rich callus induction medium. Regenerating tissues can be directed along different developmental pathways leading to the formation of shoots, new roots, or callus by transferring to the appropriate organ induction medium. Using gene-profiling methods, we identified groups of genes that serve as molecular signatures of the different developmental processes, i.e. genes that were specifically up- or down-regulated on one developmental pathway, but not on others. One transcription factor gene that was up-regulated during early shoot development was RAP2.6L (At5g13330), a member of the ERF (ethylene response factor) subfamily B-4 of the ERF/APETALA2 transcription factor gene family. RAP2.6L functions in shoot regeneration because T-DNA knockdown mutations in the gene reduced the efficiency of shoot formation in tissue culture, but not normal embryo or seedling development. RAP2.6L promoter:β-glucuronidase fusions demonstrated that the up-regulation of the gene during shoot regeneration was, at least in part, transcriptionally controlled. The promoter:β-glucuronidase fusions also demonstrated that RAP2.6L expression was localized to the shoot and emerging leaves, but expression declined in the leaf lamina as leaves expanded. T-DNA knockdown mutations in RAP2.6L reduced the expression of many genes that are normally up-regulated during shoot development including CUP-SHAPED COTYLEDON2 that is involved in shoot meristem specification. Thus, RAP2.6L appears to be part of a network involved in regulating the expression of many other genes in shoot regeneration.

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“…Em conseqüência da formação dos centros meristemáticos, a região basal dos explantes sofreu completa desestruturação, tendo alterado a composição dos tecidos internos, principalmente do parênquima cortical e do procâmbio, o que permite atribuir a esses tecidos a principal capacidade de resposta morfogênica aos tratamentos (Figura 3). Segundo Hicks (1980), a competência celular para determinadas respostas morfogenéticas in vitro pode ser intrínseca ao explante Figura 2. Número médio de brotações por explante (taxa de multiplicação) de Eucalyptus grandis, sob diferentes concentrações de BAP e tempo de exposição, após 21 dias da colocação dos explantes in vitro.…”

Section: Resultsunclassified

Multiplicação in vitro de Eucalyptus grandis sob estímulo com benzilaminopurina

Almeida¹,

Gonçalves²

2006

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar os efeitos do estímulo com benzilaminopurina (BAP) na multiplicação in vitro de Eucalyptus grandis. Foram avaliadas as interações entre concentrações de BAP (0, 200, 400 e 600 mg L -1 ), tempo de exposição (1, 2 e 3 horas), pH (3 e 5,8) da solução e alterações morfológicas dos explantes. Semanalmente, foi determinada a massa da matéria fresca dos explantes. Aos 21 dias de cultura, foram avaliados: o número de brotações por tratamento, o número de brotações obtidas por explante (taxa de multiplicação), e foi realizado o resgate das plântulas para avaliação histológica. O pH não apresentou interação com os demais fatores estudados. Os tratamentos com BAP a 200 mg L -1 , durante 1 e 2 horas, apresentaram-se como os mais indicados na multiplicação do E. grandis. Houve intensificação da divisão celular, no parênquima cortical e no procâmbio, representada pelo surgimento de meristemóides, em resposta aos tratamentos com 200 mg L -1 de BAP, durante 1 e 2 horas. O estímulo com BAP na multiplicação in vitro de E. grandis é viável.Termos para indexação: micropropagação, essência florestal, histologia, regulador vegetal. In vitro multiplication of Eucalyptus grandis under BAP pulseAbstract -The objective of this work was to evaluate the effect of pulse on in vitro multiplication of Eucalyptus grandis. Interactions were evaluated among BAP concentrations (0, 200, 400 and 600 mg L -1 ), exposure time (1, 2 and 3 hours), pH values (3 and 5.8), and explant morphological changes. Fresh weight of the explants was determined weekly. At 21 days of culture, valuations were made of the number of shootings per treatment, number of shootings obtained per explant (multiplication rate), and seedlings rescue was accomplished for histological analysis. The pH values did not present any interaction with the other factors. The most significant treatments on E. grandis shoot multiplications were 200 mg L -1 of 6-BAP during 1 and 2 hours. There was an intensification of cell division in the cortical parenchyma and procambium, represented by the arising of meristems in response to 200 mg L -1 of BAP treatment, during 1 and 2 hours. The use of the pulse on in vitro multiplication of E. grandis is feasible.

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Patterns of organ development in plant tissue culture and the problem of organ determination

Cited by 78 publications

References 59 publications

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

Gene Expression Programs during Shoot, Root, and Callus Development in Arabidopsis Tissue Culture

Multiplicação in vitro de Eucalyptus grandis sob estímulo com benzilaminopurina

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