Multiplicação in vitro de Eucalyptus grandis sob estímulo com benzilaminopurina

Almeida, Marcílio de; Gonçalves, Antônio Natal

doi:10.1590/s0100-204x2006001200005

Cited by 17 publications

(12 citation statements)

References 12 publications

(8 reference statements)

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“…Pulsing techniques involve reducing the explants' exposure time to growth regulators (Andrade et al 2006;Aasim 2010), minimizing the required time to produce micropropagated plants (Pullman et al 2003;Andrade et al 2006). This study aimed to evaluate the effect of TDZ pulsing on the in vitro peach palm morphogenesis.…”

Section: Introductionmentioning

confidence: 99%

TDZ pulsing evaluation on the in vitro morphogenesis of peach palm

Graner

Oberschelp

Brondani

et al. 2013

Physiol Mol Biol Plants

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Peach palm (Bactris gasipaes Kunth.) cropping is an excellent alternative to native species exploitation; nevertheless, the problems with seed germination and conventional propagation justify the use of in vitro culturing. Aiming to asses TDZ pulsing effect on B. gasipaes morphogenesis, explants obtained from unarmed microplants were maintained in two treatments, half of them in MS free medium (without growth regulator) and the other half in MS with TDZ (0.36 μM). Both groups were transferred to growth regulator-free MS medium following 14 days of culture. After 84 days of culture, TDZ pulsing increased the growth and development of the shoots, restricted the growth and development of the roots, with no influence on adventitious bud induction or somatic embryogenesis. Furthermore, development of prickles, thickening of roots and chlorotic leaves were noted under TDZ pulsing. Leaf sheath histological analysis showed an epidermal origin and no vascularization of these prickles.

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Section: Introductionmentioning

confidence: 99%

TDZ pulsing evaluation on the in vitro morphogenesis of peach palm

Graner

Oberschelp

Brondani

et al. 2013

Physiol Mol Biol Plants

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“…Atualmente, entre as tecnologias empregadas na otimização da produção de mudas de essências florestais, estão sendo estudados alguns produtos, sintéticos ou biológicos, que apresentam capacidade de promoção do crescimento direto ou indireto das plantas (Mafia et al, 2007). Estes produtos podem atuar a partir do fornecimento de compostos reguladores do crescimento da planta ou reduzindo os efeitos negativos às plantas proporcionados pela ação de determinados organismos patogênicos ou adversidades ambientais (Mafia et al, 2005;Andrade et al, 2006;Mafia et al, 2007).…”

Section: Introductionunclassified

Efeito estimulante do óleo essencial de eucalipto na germinação e crescimento inicial de mudas de Eucalyptus grandis

2010

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Resumo-A utilização de extratos e óleos essenciais de plantas vem sendo uma alternativa eficiente no bioestímulo do crescimento e proteção vegetal. O trabalho teve por objetivo avaliar a utilização do óleo essencial extraído de folhas de Eucalyptus grandis sobre a germinação e o desenvolvimento inicial de mudas de eucalipto em condições controladas, em casa de vegetação. Sementes de eucalipto foram incubadas em concentrações de 0, 10, 25, 50, 75 e 100 µL L-1 do óleo essencial em ambiente controlado. Posteriormente, pulverizou-se plântulas de eucalipto com 0, 20, 30, 40, 50 e 60 µL L-1 do óleo essencial por planta, em intervalos de sete dias. Avaliou-se o efeito do óleo sobre o desenvolvimento vegetal aos 30 e 60 dias após o transplante. Os resultados evidenciam que a germinação foi significativamente maior quando as sementes foram tratadas com 25 µL L-1 do óleo essencial. A aplicação do óleo essencial nas concentrações de 30 e 40 µL L-1 proporcionou maior crescimento das raízes e da parte aérea das mudas de eucalipto em ambiente controlado, sendo uma alternativa eficiente na bioestimulação do crescimento vegetativo de mudas de eucalipto. Termos para indexação: Óleos essenciais, bioestimulação, essência florestal. Stimulatory effect of eucalyptus essential oil on the germination and early growth of Eucalyptus grandis seedlings Abstract-The use of essential oil and extracts from plants is becoming an efficient alternative in the biostimulation on growth and protection of plants. The aim of this work was to evaluate the use of leaf essential oil of Eucalyptus grandis on the germination and the development of Eucalyptus grandis seedlings in nursery conditions. The eucalyptus seeds were exposed to the concentrations of 0, 10, 25, 50, 75 and 100 µL L-1 of the essential oil in controlled conditions. The eucalyptus seedlings were sprayed with 0, 20, 30, 40, 50 and 60 µL L-1 of the essential oil per plant, at intervals of seven days. The effect of this application on the seedling development were analyzed after 30 and 60 days. The results show that the germination was significantly higher when the seeds were exposed to 25 µL L-1 of the essential oil. The application of essential oil in the concentration of 30 and 40 µL L-1 provided higher growth of the aerial part and of the roots in greenhouse conditions, being an effective alternative to biostimulation the vegetative growth of eucalyptus seedlings.

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“…Media that use MS salts are usually supplemented with organic additives, including some that are constituents of MS medium (e.g., 500-555 µM myo-inositol, 1-6.25 µM thiamine-HCl, 4.06 µM nicotinic acid, 2.43 µM pyridoxine-HCl, and/or 26.64 µM glycine) and some that are not (e.g., 0.4 µM biotin and/or 0.2 µM calcium pantothenate). Other proliferation media for eucalypts have included woody plant medium (WPM) [94,97], JADS media [98][99][100], DKW medium [101], mixtures of MS and de Fossard nutrients [102], and MS basal salts with either White vitamins or B5 vitamins [85,87,90,103]. Shoot proliferation is performed in the light, usually on semi-solid medium containing 58.4 mM or 87.6 mM sucrose (Table A1).…”

Section: Shoot Culturementioning

confidence: 99%

“…However, SP medium [108,[110][111][112], WPM minerals [113], WPM [114], B5 medium [115][116][117], N7 medium [86,118], JADS medium [98], EDM [119], and MS medium with White vitamins [89] have also been used. Callus is often induced in darkness, although basal organogenesis on shoots can occur in eucalypt shoot cultures that are maintained under light [45,46,64,98,120]. Callogenesis, shoot regeneration, and shoot elongation are performed on semi-solid media, typically containing 87.6 mM or, occasionally, 58.4 mM sucrose (Table A1).…”

Section: Organogenesismentioning

confidence: 99%

Tissue Culture of Corymbia and Eucalyptus

Trueman

Hung

Wendling

2018

Forests

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Eucalypts are among the world's most widely planted trees, but the productivity of eucalypt plantations is limited by their often-low amenability to true-to-type propagation from cuttings. An alternative approach to cutting propagation is tissue culture, which can be used to micropropagate valuable genotypes rapidly while simultaneously preserving germplasm in vitro. This review describes the use of tissue culture methods such as shoot culture, organogenesis, and somatic embryogenesis for micropropagating eucalypts. This review also discusses the use of cool storage, encapsulation, and cryopreservation methods for preserving eucalypt germplasm and delaying tissue maturation under minimal-growth conditions.

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Multiplicação in vitro de Eucalyptus grandis sob estímulo com benzilaminopurina

Cited by 17 publications

References 12 publications

TDZ pulsing evaluation on the in vitro morphogenesis of peach palm

TDZ pulsing evaluation on the in vitro morphogenesis of peach palm

Efeito estimulante do óleo essencial de eucalipto na germinação e crescimento inicial de mudas de Eucalyptus grandis

Tissue Culture of Corymbia and Eucalyptus

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