Patterned neuronal networks using nanodiamonds and the effect of varying nanodiamond properties on neuronal adhesion and outgrowth

Edgington, Robert; Thalhammer, Agnes; Welch, Joseph; Bongrain, Alexandre; Bergonzo, P.; Scorsone, Emmanuel; Jackman, Richard B.; Schoepfer, Ralf

doi:10.1088/1741-2560/10/5/056022

Cited by 51 publications

(54 citation statements)

References 39 publications

(71 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Consistent with previous researches15161731, we did not observe any cytotoxic effect of FND on primary CNS or PNS neurons. Furthermore, intracranial injection of FND into the hippocampus of the rat did not alter the animal behavior.…”

Section: Discussionsupporting

confidence: 93%

“…However, a recently research showed that ND-coated surface enhanced neurite outgrowth in CNS neuron culture16. These seemingly contradicting results may be explained by the particle size of the NDs used and the attachment of NDs to the culture surface.…”

Section: Discussionmentioning

confidence: 94%

“…This result opens up the possibility of using ND to treat nervous system-related diseases and injuries. Previous researches have demonstrated the low cytotoxicity of ND in primary neurons or neurons derived from embryonal carcinoma cells151617. Thalhammer et al .…”

mentioning

confidence: 99%

“…first demonstrated that culturing mouse hippocampal neurons on ND monolayer did not affect the neuronal attachment, neurite outgrowth, or cell-autonomous neuronal excitability compared to the traditional ECM coating15. The same group recently showed that the size and curvature of the NDs exhibited a significant correlation with neurite extension, the smaller the nanodiamond the longer the neurites extend16. Other diamond or diamond-like carbon surfaces have also been tested and exhibited excellent biocompatibility with neurons1819.…”

mentioning

confidence: 99%

See 3 more Smart Citations

The effect of fluorescent nanodiamonds on neuronal survival and morphogenesis

Huang

Kao

Liu

et al. 2014

Sci Rep

View full text Add to dashboard Cite

Nanodiamond (ND) has emerged as a promising carbon nanomaterial for therapeutic applications. In previous studies, ND has been reported to have outstanding biocompatibility and high uptake rate in various cell types. ND containing nitrogen-vacancy centers exhibit fluorescence property is called fluorescent nanodiamond (FND), and has been applied for bio-labeling agent. However, the influence and application of FND on the nervous system remain elusive. In order to study the compatibility of FND on the nervous system, neurons treated with FNDs in vitro and in vivo were examined. FND did not induce cytotoxicity in primary neurons from either central (CNS) or peripheral nervous system (PNS); neither did intracranial injection of FND affect animal behavior. The neuronal uptake of FNDs was confirmed using flow cytometry and confocal microscopy. However, FND caused a concentration-dependent decrease in neurite length in both CNS and PNS neurons. Time-lapse live cell imaging showed that the reduction of neurite length was due to the spatial hindrance of FND on advancing axonal growth cone. These findings demonstrate that FNDs exhibit low neuronal toxicity but interfere with neuronal morphogenesis, and should be taken into consideration when applications involve actively growing neurites (e.g. nerve regeneration).

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 94%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

The effect of fluorescent nanodiamonds on neuronal survival and morphogenesis

Huang

Kao

Liu

et al. 2014

Sci Rep

View full text Add to dashboard Cite

show abstract

“…6,7 Diamond particles can be used for many applications in industry. 7,8 Obviously, the hardness of nano-diamond particles (ND) is related to the strong covalent bonds between the adjacent carbon atoms. [9][10][11] During anodising in their presence, the ND particles can be incorporated in the formed porosities of the anodised films, thus allowing the mechanical properties to be modified due to the high hardness of the diamond particles.…”

Section: Introductionmentioning

confidence: 99%

Study on mechanical properties of anodised film containing nano-diamond particles

Jabbari¹,

Mirjani²,

Rezagholi³

2016

Transactions of the IMF

View full text Add to dashboard Cite

Anodic-composite films containing nano-diamond particles (ND) were prepared on 7000 aluminium alloy using an anodising method. The micro-structures of the film were studied by scanning electron microscopy, energy dispersive X-ray spectroscopy, atomic force microscopy and minitab software (version 16). The results indicate that the micro-hardness and wear resistance of the films were improved using the nano-additive. Thus, the wear loss of the composite (the film anodised in the bath containing of sulphric acid, oxalic acid and 85 g L −1 ND) evaluated by dry sliding friction test for 50 000 cycles at room temperature, was 0.4 mg compared to 3 mg in the absence of ND, a 750% improvement. The friction of ND composite anodic films is significantly less than the samples without the added particles. Moreover, the micro-hardness of the composite film reached a maximum value of 770 VHN. The results also show that the mechanical properties of the composite films are mainly dependent on the structure of the oxide films.

show abstract

Monolayer Graphene Coating of Intracortical Probes for Long‐Lasting Neural Activity Monitoring

Bourrier

Shkorbatova

Bonizzato

et al. 2019

Adv Healthcare Materials

View full text Add to dashboard Cite

The invasiveness of intracortical interfaces currently used today is responsible for the formation of an intense immunoresponse and inflammatory reaction from neural cells and tissues. This leads to a high concentration of reactive glial cells around the implant site, creating a physical barrier between the neurons and the recording channels. Such a rejection of foreign analog interfaces causes neural signals to fade from recordings which become flooded by background noise after a few weeks. Despite their invasiveness, those devices are required to track single neuron activity and decode fine sensory or motor commands. In particular, such quantitative and long‐lasting recordings of individual neurons are crucial during a long time period (several months) to restore essential functions of the cortex, disrupted after injuries, stroke, or neurodegenerative diseases. To overcome this limitation, graphene and related materials have attracted numerous interests, as they gather in the same material many suitable properties for interfacing living matter, such as an exceptionally high neural affinity, diffusion barrier, and high physical robustness. In this work, the neural affinity of a graphene monolayer with numerous materials commonly used in neuroprostheses is compared, and its impact on the performance and durability of intracortical probes is investigated. For that purpose, an innovative coating method to wrap 3D intracortical probes with a continuous monolayer graphene is developed. Experimental evidence demonstrate the positive impact of graphene on the bioacceptance of conventional intracortical probes, in terms of detection efficiency and tissues responses, allowing real‐time samplings of motor neuron activity during 5 weeks. Since continuous graphene coatings can easily be implemented on a wide range of 3D surfaces, this study further motivates the use of graphene and related materials as it could significantly contribute to reduce the current rejection of neural probes currently used in many research areas, from fundamental neurosciences to medicine and neuroprostheses.

show abstract

Patterned neuronal networks using nanodiamonds and the effect of varying nanodiamond properties on neuronal adhesion and outgrowth

Abstract: The results of nanoparticle size and curvature being influential upon neuronal adhesion has great implications towards biomaterial design, and the ability to pattern neurons using nanodiamond tracks shows great promise for applications both in vitro and in vivo.

Cited by 51 publications

References 39 publications

The effect of fluorescent nanodiamonds on neuronal survival and morphogenesis

The effect of fluorescent nanodiamonds on neuronal survival and morphogenesis

Study on mechanical properties of anodised film containing nano-diamond particles

Monolayer Graphene Coating of Intracortical Probes for Long‐Lasting Neural Activity Monitoring

Contact Info

Product

Resources

About