Pathophysiology of Haemonchus placei infection in calves

Gennari, Solange Maria; Bressan, Maria Cecília Reale Vieira; Rogero, J.R.; Maclean, John; Duncan, J.L.

doi:10.1016/0304-4017(91)90126-g

Cited by 21 publications

(6 citation statements)

References 12 publications

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“…Gastrointestinal nematodiasis (GIN) substantially affects cattle health, particularly in cases of co-infection by infectious and parasitic agents [1]. Among parasitic species of veterinary concern, the hematophagous nematode Haemonchus placei is particularly pathogenic to cattle in tropical regions, causing hypoproteinemia, anemia, and anorexia in host animals [2], often with considerable economic losses.…”

Section: Introductionmentioning

confidence: 99%

Discovery of potential ovicidal natural products using metabolomics

et al. 2019

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Plant extracts are a potential source of new compounds for nematode control and may be an excellent alternative for the control gastrointestinal nematodes that are resistant to conventional anthelmintics. However, research involving natural products is a complex process. The main challenge is the identification of bioactive compounds. Online analytical techniques with universal detectors, such as high-performance liquid chromatography-mass spectrometry (HPLC-MS), together with metabolomics could enable the fast, accurate evaluation of a massive amount of data, constituting a viable option for the identification of active compounds in plant extracts. This study focused on the evaluation of the ovicidal activity of ethanol extracts from 17 plants collected from the Pantanal wetland in the state of Mato Grosso do Sul, Brazil, against eggs of Haemonchus placei using the egg hatchability test. The ethanol extracts were obtained using accelerated solvent extraction. The data on ovicidal activity, mass spectrometry and metabolomics were evaluated using HPLC-DAD-MS, partial least squares regression analysis (PLS-DA) and a correlation map (univariate correlation analyses) to detect compounds that have a positive correlation with biological activity. Among the ten metabolites with the best correlation coefficients, six were phenylpropanoids, two were triterpene saponins, one was a brevipolide, and one was a flavonoid. Combinations of metabolites with high ovicidal action were also identified, such as phenylpropanoids combined with the triterpene saponins and the flavonoid, flavonoids combined with iridoid and phenylpropanoids, and saponins combined with phenylpropanoid. The positive correlation between classes of compounds in plants belonging to different genera and biological activity (as previously identified in the literature) reinforces the robustness of the statistical data and demonstrates the efficacy of this method for the selection of bioactive compounds without the need for isolation and reevaluation. The proposed method also enables the determination of synergism among the classes, which would be impracticable using traditional methods. The present investigation demonstrates that the metabolomic technique was efficient at detecting secondary metabolites with ovicidal activity against H. placei. Thus, the use of metabolomics can be a tool to accelerate and simplify bioprospecting research with plant extracts in veterinary parasitology.

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Section: Introductionmentioning

confidence: 99%

Discovery of potential ovicidal natural products using metabolomics

et al. 2019

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“…A verminose se apresenta sob a forma aguda, levando os animais rapidamente à morte; ou sob a forma crônica, em que os efeitos do parasitismo são gradativamente notados, tais como menor desenvolvimento corporal, perda de peso, redução na produção e na qualidade de lã, má eficiência reprodutiva, reduzida resistência a enfermidades e elevado índice de mortalidade, principalmente entre os animais jovens (Jardim 1974, Hammerberg 1986, Holmes 1987, Gennari et al 1991. Echevarria (1988) relatou que a verminose em ovinos pode reduzir de 20 a 60% o ganho de peso e ocasionar uma taxa de mortalidade que pode variar de 20 a 40% dos rebanhos.…”

Section: Introductionunclassified

Resistência anti-helmíntica de nematóides gastrintestinais em ovinos, Mato Grosso do Sul

Sczesny-Moraes

Bianchin²,

Silva³

et al. 2010

Pesq. Vet. Bras.

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ABSTRACT.-Sczesny-Moraes E.A., Bianchin I., Silva K.F., Catto J. Among the methods of control of gastrointestinal worms in sheep, the use of chemicals is the most common. However, the continued, and indiscriminate, use of these products has selected populations of resistant helminths to anthelmintics, a phenomenon reported in the whole world. This study aimed to identify the species of gastrointestinal parasites and diagnose the status of anthelmintic resistance in sheep in the State of Mato Grosso do Sul Brazil. Feacal egg count reduction tests (FECRT) were performed in flocks of sixteen farms, and the seven formulations used contained the following pharmacological bases: Albendazole, Ivermectin, Levamizol, Trichlorfon, Moxidectin, Closantel and one containing the first three in association. The species identified at necropsy, in adult sheep, were: Haemonchus contortus, Trichostrongylus colubriformis, Cooperia curticei, C. punctata, C. pectinata and Oesophagostomum columbianum, in order of prevalence. The formulations containing Albendazole and Ivermectin did not show efficacy in reducing the EPG in the flocks tested, with average reductions of 0.7 and -19.6%, respectively. Closantel presented an average efficacy of 6.7%; Levamisolee, Moxidectin and Trichlorfon, 28.7, 26.8 and 65% respectively, the combination of three bases (Albendazole, Ivermectin and Levamizol), an average efficacy of 55.8 %. The average percentages of infective larvae recovered in the faecal cultures, pre and post treatment were similar, indicating that resistance to the bases tested is present in all species cited, to a greater or lesser degree. The two genera predominantly resistant are Haemonchus sp., with 86.9%, followed by Trichostrongylus sp., with an average of 47.5%, Strongyloides sp. 33.6%, Oesophagostomum sp.

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“…No changes in iron and UIBC were found, although hematophagous parasites such as Haemonchus spp. could produce changes in iron and UIBC (Gennari et al, 1991). In addition total protein, albumin and globulin did not change in the parasitized animals.…”

Section: Tablementioning

confidence: 82%