Pathogenesis in Pseudomonas aeruginosa PAO1 Biofilm-Associated Is Dependent on the Pyoverdine and Pyocyanin Siderophores by Quorum Sensing Modulation

Díaz-Pérez, Sharel Pamela; Solis, Christian Said; López‐Bucio, Jesús Salvador; Alarcón, Juan José; Villegas, Javier; Cruz, Homero Reyes de la; Campos-Garcı́a, Jesús

doi:10.1007/s00248-022-02095-5

Cited by 17 publications

(11 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4A). Among the proteins depleted in Δ plaF , the strongest effect was observed for the proteins constituting the pathway facilitating iron uptake by pyoverdine, a major iron-binding chelator in P. aeruginosa , [75, 76] (Fig. 4B).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Virulence adaptation ofPseudomonas aeruginosaphospholipase mutant with altered membrane phospholipid composition

Caliskan

Poschmann

Gudzuhn

et al. 2022

Preprint

View full text Add to dashboard Cite

Membrane protein and phospholipid (PL) composition changes in response to environmental cues and during infections. Covalent modification and remodelling of the acyl chain length of PLs is an important bacterial adaptation mechanism. However, little is known about which bacterial pathways are regulated in response to altered PL composition. Here, we showed that P. aeruginosa phospholipase A, PlaF, which modulates membrane PL composition, is important for biofilm biogenesis, and we performed whole-cell quantitative proteomics of P. aeruginosa wild-type and delta plaF biofilms to identify pathways regulated by PlaF. The results revealed profound alterations in the abundance of several two-component systems (TCSs), including accumulation of PprAB, which controls the transition to biofilm. Furthermore, a unique phosphorylation pattern of transcriptional regulators, transporters and metabolic enzymes, as well as differential production of seven proteases, in delta plaF, indicate that PlaF-mediated virulence adaptation involves complex transcriptional and posttranscriptional regulation. Moreover, proteomics revealed the depletion of pyoverdine-mediated iron uptake pathway proteins in delta plaF, which agrees with the decreased concentrations of extracellular pyoverdine and intracellular iron and is likely responsible for its prolonged lag growth phase, presumably due to reduced iron uptake. Conversely, the accumulation of proteins from alternative iron-uptake systems in delta plaF suggests that PlaF may function as a switch between different iron-acquisition pathways. The observation that ∆plaF accumulates PL-acyl chain modifying and PL synthesis enzymes reveals novel insights into the role of PlaF for membrane PL homeostasis. Although the precise mechanism by which PlaF simultaneously affects multiple pathways remains to be elucidated, we suggest that PlaF-catalyses the degradation of PLs which then serve as a signal that is amplified by proteins of two-component, phosphorylation and proteolytic degradation systems to elicit the global adaptive response in P. aeruginosa.

show abstract

Section: Resultsmentioning

confidence: 99%

“…The interdependence of iron acquisition, biofilm formation and virulence is well documented in P. aeruginosa [56, 75, 117] and other major pathogens [25, 118, 119]. However, understanding the relationship between iron acquisition and membrane PL composition in bacteria is limited.…”

Section: Discussionmentioning

confidence: 99%

Virulence adaptation ofPseudomonas aeruginosaphospholipase mutant with altered membrane phospholipid composition

Caliskan

Poschmann

Gudzuhn

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…6 Quorum sensing (QS) modulators Parasitic microorganisms, particularly exemplified by Pseudomonas aeruginosa, deploy adaptive strategies, such as the quorum sensing (QS) controlled formation of biofilms and the production of countless, structurally diverse virulence factors (Díaz-Pérez et al, 2022). This enables the bacteria to function in a synchronized fashion (Lazar et al, 2021) which allows them to readily adapt to environmental changes and protect the biofilm integrity.…”

Section: Fk506mentioning

confidence: 99%

Late-stage diversification of bacterial natural products through biocatalysis

Lazic,

Filipovic,

Pantelic

et al. 2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Bacterial natural products (BNPs) are very important sources of leads for drug development and chemical novelty. The possibility to perform late-stage diversification of BNPs using biocatalysis is an attractive alternative route other than total chemical synthesis or metal complexation reactions. Although biocatalysis is gaining popularity as a green chemistry methodology, a vast majority of orphan sequenced genomic data related to metabolic pathways for BNP biosynthesis and its tailoring enzymes are underexplored. In this review, we report a systematic overview of biotransformations of 21 molecules, which include derivatization by halogenation, esterification, reduction, oxidation, alkylation and nitration reactions, as well as degradation products as their sub-derivatives. These BNPs were grouped based on their biological activities into antibacterial (5), antifungal (5), anticancer (5), immunosuppressive (2) and quorum sensing modulating (4) compounds. This study summarized 73 derivatives and 16 degradation sub-derivatives originating from 12 BNPs. The highest number of biocatalytic reactions was observed for drugs that are already in clinical use: 28 reactions for the antibacterial drug vancomycin, followed by 18 reactions reported for the immunosuppressive drug rapamycin. The most common biocatalysts include oxidoreductases, transferases, lipases, isomerases and haloperoxidases. This review highlights biocatalytic routes for the late-stage diversification reactions of BNPs, which potentially help to recognize the structural optimizations of bioactive scaffolds for the generation of new biomolecules, eventually leading to drug development.

show abstract

“…In a recent study, Díaz‐Pérez et al. (2022) demonstrated that the pathogenesis of P. aeruginosa PAO1 was dependent on the siderophores regulated by QS systems.…”

Section: Bacterial Qs Systems and The Food Industrymentioning

confidence: 99%

Quorum‐quenching enzymes: Promising bioresources and their opportunities and challenges as alternative bacteriostatic agents in food industry

Dangfeng

Cui

Ren

et al. 2023

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

The problems of spoilage, disease, and biofilm caused by bacterial quorum‐sensing (QS) systems have posed a significant challenge to the development of the food industry. Quorum‐quenching (QQ) enzymes can block QS by hydrolyzing or modifying the signal molecule, making these enzymes promising new candidates for use as antimicrobials. With many recent studies of QQ enzymes and their potential to target foodborne bacteria, an updated and systematic review is necessary. Thus, the goals of this review were to summarize what is known about the effects of bacterial QS on the food industry; discuss the current understanding of the catalytic mechanisms of QQ enzymes, including lactonase, acylase, and oxidoreductase; and describe strategies for the engineering and evolution of QQ enzymes for practical use. In particular, this review focuses on the latest progress in the application of QQ enzymes in the field of food. Finally, the current challenges limiting the systematic application of QQ enzymes in the food industry are discussed to help guide the future development of these important enzymes.

show abstract

Pathogenesis in Pseudomonas aeruginosa PAO1 Biofilm-Associated Is Dependent on the Pyoverdine and Pyocyanin Siderophores by Quorum Sensing Modulation

Cited by 17 publications

References 60 publications

Virulence adaptation ofPseudomonas aeruginosaphospholipase mutant with altered membrane phospholipid composition

Virulence adaptation ofPseudomonas aeruginosaphospholipase mutant with altered membrane phospholipid composition

Late-stage diversification of bacterial natural products through biocatalysis

Quorum‐quenching enzymes: Promising bioresources and their opportunities and challenges as alternative bacteriostatic agents in food industry

Contact Info

Product

Resources

About