Patatin, the Tuber Storage Protein of Potato (<i>Solanum tuberosum</i> L.), Exhibits Antioxidant Activity in Vitro

Liu, Yen Wenn; Han, Chuan Hsiao; Lee, Mei Hsien; Hsu, Feng‐Lin; Hou, Wen Chi

doi:10.1021/jf030016j

Cited by 110 publications

(83 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Patatin has also been shown to possess antioxidant or antiradical activity. Liu and colleagues [12] found that purified patatin exert antioxidant or antiradical activity in various in vitro tests, such as radical, scavenging activity assay and protection against hydroxyl radical-induced calf thymus DNA damage. Potato protein hydrolysates showed antioxidant activity [13] and enhanced oxidative stability of soybean oil emulsions [14].…”

Section: Biological Activities Of Plant Proteinsmentioning

confidence: 99%

Antihypertensive Properties of Plant Protein Derived Peptides

Pihlanto¹,

Mäkinen²

2013

Bioactive Food Peptides in Health and Disease

View full text Add to dashboard Cite

Section: Biological Activities Of Plant Proteinsmentioning

confidence: 99%

Antihypertensive Properties of Plant Protein Derived Peptides

Pihlanto¹,

Mäkinen²

2013

Bioactive Food Peptides in Health and Disease

View full text Add to dashboard Cite

“…Potato storage proteins provide necessary nutrients for the development of tuber, mature-tosprouting tuber transition and successful plant growth [1][2][3][4]. The patatin is the major VSP of S. tuberosum, accounting for up to 45% of the total soluble protein [1,[4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…The patatin is the major VSP of S. tuberosum, accounting for up to 45% of the total soluble protein [1,[4][5][6][7]. However, the molecular mechanism that triggers the cleavage of the patatin along the tuber life cycle has not to date been identified.…”

Section: Introductionmentioning

confidence: 99%

Identification and Mapping of Phosphorylated Isoforms of the Major Storage Protein of Potato Based on Two- Dimensional Electrophoresis

Bernal¹,

López‐Pedrouso²,

Daprà³

et al. 2017

Advances in Seed Biology

View full text Add to dashboard Cite

Protein phosphorylation plays a key role in the synthesis and degradation of dry seed storage proteins. In contrast, no evidence for phosphorylation has been reported to date in vegetative storage proteins (VSPs). The patatin multigene family encodes the major VSP of the potato, Solanum tuberosum L. This study addresses for the first time the identification and mapping of phosphorylated patatin forms based on high-resolution two-dimensional electrophoresis (2-DE) profiles. Patatin isoforms from mature tubers of cultivar Kennebec were separated by 2-DE and subsequently identified by tandem mass spectrometry. In-gel identification and mapping of phosphorylated isoforms were performed using the multiplex phosphoprotein-specific staining Pro-Q DPS. We found that phosphorylation is a ubiquitous post-translational protein modification associated with isoforms of patatin. In addition, protein dephosphorylation with hydrogen fluoride-pyridine coupled to 2-DE was used for quantitative profiling of phosphorylated patatin. This experimental approach showed that patatin comprises multiple isoforms with very different phosphorylation level. Thus, phosphorylation rates over isoforms ranged from 4.6 to 52.3%. Overall, the identification and mapping of differentially phosphorylated patatin opens up new exploratory ways to unravel the molecular mechanisms underlying its mobilization along the tuber life cycle.

show abstract

“…Also several proteins are induced by diverse types of stress (Mehta et al, 1991) and similarly as phenols generate antiradical activities, i.e. health promoting effects in humans when consumed (Grace, 2005;Liu et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Influence of Drought and Wounding Stress on Soluble Phenols and Proteins in Potato Tubers

Wegener¹,

Jansen²,

Jürgens³

2014

SAR

View full text Add to dashboard Cite

Potato is a valuable source for phytochemicals like vitamins, minerals, plant phenols, amino acids and proteins. However, environmental stress may affect the level of all these components. In this study, two purple breeding clones and one yellow fleshed cultivar (cv.) were assessed in their response to drought and wounding stress in two consecutive years. The plants were grown in the greenhouse under control (sufficient water supply) and with drought stress conditions. After harvest the tubers were analyzed for the content of soluble phenols and proteins in fresh tissue and after wounding. In addition, free amino acids (AAS) and activity of lipid acyl hydrolases (LAH) were assayed in control and drought stressed tubers. The results revealed significant differences in soluble phenols and proteins between genotypes, and that drought stress significantly increased the level of soluble proteins (P < 0.0001) and LAH activity (P < 0.001), but had no significant effect on the concentration of phenols. Moreover, total amounts of free AAS were higher in the drought stress variant. Wounding stress caused a significant increase of soluble phenols in cv. Agave. But, this was less prominent in purple clones which in general had higher contents of phenols. Proteins were also enhanced as a result of wounding, although, the effect of wounding stress on their level was smaller than that of drought stress.

show abstract

Patatin, the Tuber Storage Protein of Potato (Solanum tuberosum L.), Exhibits Antioxidant Activity in Vitro

Cited by 110 publications

References 23 publications

Antihypertensive Properties of Plant Protein Derived Peptides

Antihypertensive Properties of Plant Protein Derived Peptides

Identification and Mapping of Phosphorylated Isoforms of the Major Storage Protein of Potato Based on Two- Dimensional Electrophoresis

Influence of Drought and Wounding Stress on Soluble Phenols and Proteins in Potato Tubers

Contact Info

Product

Resources

About