Influence of Drought and Wounding Stress on Soluble Phenols and Proteins in Potato Tubers

Wegener, Christina; Jansen, Gisela; Jürgens, Hans‐Ulrich

doi:10.5539/sar.v3n3p1

Cited by 15 publications

(17 citation statements)

References 68 publications

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“…The results of this study revealed significantly higher LAH activities in drought stressed tubers than in the control tubers (Table 3A). An increase in LAH by DS was consistently found with all GTs, in the first (+42.8%) and second year (+54.7%), which supports the results of previous experiments [32].…”

Section: Protein Myo-inositol and Fatty Acidssupporting

confidence: 91%

“…The analytical block digestion system Kjeldatherm®, the distillation system VAPODEST® both from C. Gerhard, Germany and the TitroLine easy apparatus from Schott Instruments, Germany were combined for the determination of crude protein by means of the Kjeldahl method [30]. Lipid acyl hydrolase (LAH) enzyme activity was measured on a UV spectrophotometer from Kontron Instruments, Germany according to Bohac [31] with modifications [32]. Extraction of total tuber lipids was performed as detailed by Bligh and Dyer [33] before fatty acids were analyzed as fatty acid methyl ester (FAME) on an Agilent 6890A GC with FID (Agilent Technologies, USA) as described by Arens et al [34].…”

Section: Analysesmentioning

confidence: 99%

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Drought stress affects bioactive compounds in potatoes (Solanum tuberosum L.) relevant to non-communicable diseases

Wegener¹,

Jürgens²,

Jansen³

2017

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Background: Potatoes react very sensitive to drought during growth, and appropriate plant stress responses may affect metabolites associated with the health quality of tubers.Objective: The aim of this study is to determine the effects of drought stress (DS) on soluble sugars, starch, crude protein, minerals, free amino acids (AAs) and fatty acids (FAs).Design: The experiment was carried out on three potato genotypes during two years with four replications. The plants were grown in pots, in a glasshouse with optimal water supply and under drought stress conditions. After harvest, the tubers of these two variants were analyzed for bioactive compounds relevant to non-communicable diseases (NCDs). Results: Apart from genotypic differences in most parameters, the results revealed that the DS caused a decline in glucose and fructose (P < 0.05, all) in both years, whereas sucrose was increased, especially in the second year with severe stress (P < 0.01). Starch was significantly reduced by moderate stress in the first year (P < 0.01), but less affected in the second year. Crude proteins and total amounts of free amino acids (AAs) were clearly enhanced by the stress in both years (P < 0.05, all), similarly as the minerals magnesium (Mg), potassium (K) and phosphorus (P) (P < 0.05, all), whereas calcium (Ca) declined (P < 0.05). The portion of α-linolenic acid (ALA) on total lipids was elevated in the stress variants (P < 0.01), while oleic acid (OLA), its precursor decreased significantly (P < 0.05), but only in the first year. In the second year, ALA was in general higher and not further induced by the stress. Also OLA was less affected in that year, similarly as all other FAs in both years. Interestingly, Myo-inositol (MI) and lipid acyl hydrolases (LAH) associated with modulation in cell membrane lipids raised by the drought stress in each year (P < 0.01, all). In the second year, MI and LAH data of the drought stressed tubers correlated significantly (r = 0.90, P < 0.01) indicating their joined action within plant stress responses.Conclusions: The biochemical changes induced by DS are not alarming with regard to NCDs. Decline in glucose, fructose and starch, and increase in crude proteins, free AAs, ALA, MI and minerals like Mg, K and P, on the other side, is profitable for the health benefits of tubers. However, a better quality is associated with a decrease in tuber yield.Keywords: potato, drought stress, bioactive compounds, chronic diseases

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Section: Protein Myo-inositol and Fatty Acidssupporting

confidence: 91%

Section: Analysesmentioning

confidence: 99%

Drought stress affects bioactive compounds in potatoes (Solanum tuberosum L.) relevant to non-communicable diseases

Wegener¹,

Jürgens²,

Jansen³

2017

FFHD

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“…Analyses: Lyophilized tissue samples were used for the analyses of anthocyanins according to Fuleci and Francis [16] and for the assay of LAH activity according to Bohac [5], both with modifications [17]. Total amounts of Ac were measured at 545 nm on a UV spectrometer (Kontron Instruments, Germany), then calculated as malvidin-3-p-coumaryl-glucoside and expressed as milligrams per 100 grams freeze-dried matter (FDM).…”

Section: Drought Stressmentioning

confidence: 99%

“…Amounts of proteins were expressed as mg/mL of tissue extract. Total amounts of free AAS were measured by the method of Cohen & Michaud [21] adapted to a Luna C18 (2) bonded silica column (Phenomenex, Germany) according to Hernández-Orte et al [22] with modifications [17]. The HPLC assay was carried out on an Agilent 1100 series liquid chromatograph system (Agilent Technologies, Germany), and amounts of AAS were expressed in milligrams per 100 g of FW.…”

Section: Drought Stressmentioning

confidence: 99%

Bioactive compounds in potatoes: Accumulation under drought stress conditions

Wegener

Jansen

Jürgens

2015

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Background: Potato (Solanum tuberosum) is a valuable source of bioactive compounds. Besides starch, crude fibre, amino acids (AAS), vitamins and minerals, the tubers contain diverse phenolic compounds. These phenolics and AAS confer anti-oxidant protection against reactive oxygen species, tissue damage, and diseases like atherosclerosis, renal failure, diabetes mellitus, and cancer. Climate change and drought stress may become a major risk for crop production worldwide, resulting in reduced access for those who depend on the nutritional value of this staple crop.Objective: The aim of this study is to determine the effect of drought stress on water, lipid soluble antioxidants, anthocyanins (Ac), soluble phenols, proteins, free AAS, peroxidase (POD) and lipid acyl hydrolase activity (LAH) in tuber tissue.Methods: The study was carried out on three potato genotypes comprising one yellow-fleshed cultivar and two purple breeding clones. The plants were grown in pots (from April to September) in a glasshouse with sufficient water supply and under drought stress conditions. After harvest, the tubers of both variants were analysed for antioxidants measured as ascorbic acid (ACE) and Trolox equivalent (TXE) using a photo-chemiluminescent method. Amounts of anthocyanins (Ac), soluble phenols, proteins, as well as POD and LAH activities were analysed using a UV photometer. Finally, free AAS were measured by HPLC.Results: The results revealed that drought stress significantly reduces tuber yield, but has no significant effect on antioxidants, Ac, soluble phenols and POD. Drought stress significantly increased the levels of soluble protein (P < 0.0001) and LAH (P < 0.001). Also, total amounts of Functional Foods in Health and Disease 2015; 5(3): 108-116 Page 109 of 116 free AAS were higher in the drought stressed tubers (+34.2%, on average) than in the tubers grown with a sufficient water supply. Above all, proline was elevated due to drought stress.Key words: anthocyanins, antioxidants, free amino acids, phenols, proteins, tuber quality

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“…SP Mafakheri et al, 2011), Chl (Ma et al, 2004;Zaefyzadeh et al, 2009), RWC (Golparvar, 2012;Teulat et al, 2003), RWL (Zhang et al, 2004), MDA (Guan et al, 2015), SOD and POD activities (Yang et al, 2014;Weng et al, 2015) which are closely associated with drought tolerance can be used as reference indexes for evaluation of drought resistance among varieties. And these indexes have been widely utilized for the study of maize, potato, soybean and wheat drought resistance (Bano et al, 2012;Mohammadkhani and Heidari, 2008;Paknejad et al, 2007;Wegener, et al, 2014;Zhang et al, 2007b).…”

Section: Changes In Physiological Indexes In Response To Drought Stressmentioning

confidence: 99%

Evaluation of drought resistance in Iris germanica L. based on subordination function and principal component analysis

Wei

Qin

et al. 2017

Emir J Food Agric

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Drought is one of the major environment stresses that have a wide range of impact on plants. In this study, seven physiological indexes including the content of soluble protein (SP), chlorophyll (Chl) and malondialdehyde (MDA), superoxide dismutase (SOD) and peroxidase (POD) activities, leaf relative water content (RWC), rate of water loss (RWL) from excised leaves were measured in leaves of Iris germanica before and after the drought treatment. It was found that the content of MDA and SP, POD and SOD activity increased, while RWL and RWC decreased in response to drought stress. Based on the subordinate function values of seven physiological indexes, seven single indexes were transformed into three principal components namely damage degree, active oxygen removal ability and moisture condition and the composite score (F value) of each iris variety was calculated by principal component analysis (PCA). Based on the F values, 10 iris cultivars could be divided into three groups by cluster analysis (CA): drought-resistance (2 varieties), medium drought-resistance (5 varieties), and low drought-resistance (3 varieties). Meanwhile, optimum regression equation was constructed. Therefore, this work provides a comprehensive and reliable method for evaluating drought resistance in the varieties of Iris germanica.

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Influence of Drought and Wounding Stress on Soluble Phenols and Proteins in Potato Tubers

Cited by 15 publications

References 68 publications

Drought stress affects bioactive compounds in potatoes (Solanum tuberosum L.) relevant to non-communicable diseases

Drought stress affects bioactive compounds in potatoes (Solanum tuberosum L.) relevant to non-communicable diseases

Bioactive compounds in potatoes: Accumulation under drought stress conditions

Evaluation of drought resistance in Iris germanica L. based on subordination function and principal component analysis

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