Pasteurella species isolated from the bovine respiratory tract and their antimicrobial sensitivity patterns

Allan, EM; Wiseman, A; Gibbs, H. A.; Selman, IE

doi:10.1136/vr.117.24.629

Cited by 31 publications

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“…The overall high detection rate of P. multocida (57.4%) in the nasopharynx of apparently healthy cattle is in contrast with other reports where it was found in only 0.5% of 971 (Allan et al, 1985), and 0% of 40 (Barbour et al, 1997) nasal swabs. This is probably due to the disinfection of the nostril and the use of selective medium in the present study, which minimized contamination of the samples by organisms other than Pasteurellaceae.…”

Section: Discussioncontrasting

confidence: 82%

Detection of Tetracycline-Resistant and Susceptible Pasteurellaceae in the Nasopharynx of Loose Group-Housed Calves

et al. 2006

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The aim of the present study was to determine which Pasteurella and Mannheimia species are present in the upper respiratory tract of healthy calves with no history of antimicrobial treatment prior to sampling. The presence of subpopulations of tetracycline-resistant Pasteurellaceae was also investigated. Nasal swabs from 61 loose group-housed, clinically healthy calves, 1 to 4 months old, from 16 dairy herds were inoculated aerobically on a selective medium (Columbia agar with 5% ovine blood and 16 mg/L bacitracin) with or without 4 mg/L oxytetracycline (OTC). A total of 43 strains belonging to the family Pasteurellaceae were isolated from 38 calves (62.3%) out of 13 herds (81.3%). The predominant organisms were Pasteurella multocida subsp. multocida (57.4%), Mannheimia varigena (4.9%) and M. haemolytica (3.2%). Growth of Pasteurellaceae on the OTC-containing medium was seen only with samples from two herds (6 animals; 9.8%), and on only one farm this proved to be an OTC-resistant subpopulation. Minimum inhibitory concentration (MIC) determinations by means of agar dilution confirmed a low prevalence of OTC-resistant Pasteurellaceae, with overall MIC(50) and MIC(90) values of 0.25 and 32 mg/L, respectively. These data do not support the hypothesis that the relative high frequency of tetracycline-resistant P. multocida isolates from fatal cases of bovine respiratory disease is related to the presence of minor tetracycline-resistance subpopulations within this species.

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Section: Discussioncontrasting

confidence: 82%

Detection of Tetracycline-Resistant and Susceptible Pasteurellaceae in the Nasopharynx of Loose Group-Housed Calves

et al. 2006

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“…Katoh et al [5] have shown that M. haemolytica isolates were susceptible to aminobenzylpenicillin, thiamphenicol, and enrofloxacine. Resistances to penicillin antibiotic of M. haemolytica were prevalent among cattle in North America and England [1,4,12]. This study showed that resistance to penicillin antibiotics (2 isolates) and thiamphenicol (2 isolates) was detected in Japanese isolates.…”

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confidence: 86%

“…Mannheimia haemolytica, which was previously described as Pasteurella haemolytica biotype A, is a causative pathogen of pneumonic pasteurellosis in calves and also isolated from the respiratory tract of cattle with BRDC, as well as P. multocida and Haemophilus somnus [1,10]. Although viral and bacterial vaccines have been used for preventing the individual diseases, controlling BRDC is usually difficult because BRDC is multifactorial disease.…”

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confidence: 99%

Antimicrobial Susceptibility of Mannheimia haemolytica Isolates from Cattle in Japan from 2001 to 2002

Esaki

Asai

Kojima

et al. 2005

The Journal of Veterinary Medical Science

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ABSTRACT. A total of 27 clinical isolates of Mannheimia haemolytica from cattle in Japan from 2001 to 2002 were examined for antimicrobial susceptibility to 25 antimicrobial agents. The minimum inhibitory concentrations of 25 different antimicrobials were determined by an agar dilution method according to the guidelines of the National Committee for Clinical Laboratory Standards. Of the 27 isolates, seven isolates (26.9%) were resistant to at least one of the 25 drugs and resistance rates ranged from 3.7 to 18.5%. Resistance rates to dihydrostreptomycin (18.5%), oxytetracycline (11.1%), and doxycycline (11.1%) were relatively high and those to the remaining drugs were less than 10%.

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“…Bovine pneumonic pasteurellosis (BPP) caused by Mannheimia (Pasteurella) haemolytica serotype 1 remains a major economic problem for the beef and dairy cattle industries in North America and Western Europe (2,10,14,47). The leukotoxin (LktA) produced by this bacterium is the primary virulence factor that contributes to the pathogenesis of the fibrinonecrotizing pleuropneumonia and death characteristic of this disease (7,8,43,44).…”

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confidence: 99%

Mannheimia haemolyticaLeukotoxin Activates a Nonreceptor Tyrosine Kinase Signaling Cascade in Bovine Leukocytes, Which Induces Biological Effects

et al. 2001

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The leukotoxin (LktA) produced by Mannheimia haemolytica binds to bovine lymphocyte function-associated antigen 1 (LFA-1) and induces biological effects in bovine leukocytes in a cellular and species-specific fashion. We have previously shown that LktA also binds to porcine LFA-1 without eliciting any effects. These findings suggest that the specificity of LktA effects must entail both binding to LFA-1 and activation of signaling pathways which are present in bovine leukocytes. However, the signaling pathways leading to biological effects upon LktA binding to LFA-1 have not been characterized. In this context, several reports have indicated that ligand binding to LFA-1 results in activation of a nonreceptor tyrosine kinase (NRTK) signaling cascade. We designed experiments with the following objectives: (i) to determine whether LktA binding to LFA-1 leads to activation of NRTKs, (ii) to examine whether LktA-induced NRTK activation is target cell specific, and (iii) to determine whether LktA-induced NRTK activation is required for biological effects. We used a biologically inactive mutant leukotoxin ( Bovine pneumonic pasteurellosis (BPP) caused by Mannheimia (Pasteurella) haemolytica serotype 1 remains a major economic problem for the beef and dairy cattle industries in North America and Western Europe (2, 10, 14, 47). The leukotoxin (LktA) produced by this bacterium is the primary virulence factor that contributes to the pathogenesis of the fibrinonecrotizing pleuropneumonia and death characteristic of this disease (7,8,43,44). A large body of evidence indicates that much of the lung injury in this disease is caused by inflammatory mediators released from alveolar leukocytes by LktA-induced activation and cytolysis (11,34,41,48).LktA is a member of a family of gram-negative RTX (repeats in toxin) cytolysins (12,20). Unlike most other RTX cytolysins, leukotoxins produced by Actinobacillus actinomycetemcomitans (LtxA) and M. haemolytica (LktA) demonstrate cell-type-specific and species-specific biological effects. The LtxA of A. actinomycetemcomitans, which causes dental caries in humans, interacts only with cells of the lymphocytic and monomyelocytic lineages of humans and some nonhuman primates and provokes biological effects (29); the LktA of M. haemolytica interacts only with ruminant leukocytes and platelets and induces biological effects (6,25,37). A study by Lally et al. (30) reported that human lymphocyte function-associated antigen 1 (LFA-1), a member of the ␤2 integrins, is a target cell receptor for LtxA of A. actinomycetemcomitans. Three studies have identified bovine CD18, the ␤ subunit of all three bovine ␤2 integrins (LFA-1, Mac-1, and p150,95), as a receptor for M. haemolytica LktA (3,33,42). However, in these studies no specific member of the ␤2 integrin family was identified as an LktA receptor. We have extended these observations and have shown that bovine LFA-1, but not other members of the ␤2 integrin family, is a receptor for M. haemolytica LktA (23).LFA-1 is a heterodimeric glycoprotein co...

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Pasteurella species isolated from the bovine respiratory tract and their antimicrobial sensitivity patterns

Cited by 31 publications

References 0 publications

Detection of Tetracycline-Resistant and Susceptible Pasteurellaceae in the Nasopharynx of Loose Group-Housed Calves

Detection of Tetracycline-Resistant and Susceptible Pasteurellaceae in the Nasopharynx of Loose Group-Housed Calves

Antimicrobial Susceptibility of Mannheimia haemolytica Isolates from Cattle in Japan from 2001 to 2002

Mannheimia haemolyticaLeukotoxin Activates a Nonreceptor Tyrosine Kinase Signaling Cascade in Bovine Leukocytes, Which Induces Biological Effects

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