Detection of Tetracycline-Resistant and Susceptible Pasteurellaceae in the Nasopharynx of Loose Group-Housed Calves

Catry, Boudewijn; Decostere, Annemie; Schwarz, Štefan; Kehrenberg, Corinna; Kruif, Aart de; Haesebrouck, Freddy

doi:10.1007/s11259-006-3347-8

Cited by 28 publications

(28 citation statements)

References 19 publications

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“…Increase in resistance against antibiotics in both P. multocida and M. haemolytica isolates have been reported [27,28]. In our study, according to the antimicrobial susceptibility test results, chloramphenicol (100%), sulfamethoxazole (89.1%) and tetracycline (84.4%) were the most effective drugs, whereas ampicilin (53.1%) was the only intermediate drug.…”

Section: Discussionsupporting

confidence: 53%

A study on Ovine pneumonic pasteurellosis: Isolation and Identification of Pasteurellae and their antibiogram susceptibility pattern in Haramaya District, Eastern Hararghe, Ethiopia

2013

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BackgroundSheep constitute the second major component of livestock in Ethiopia. However, efficient utilization of this potential resource is hampered by combination of health problems, poor management and feed shortage. Haramaya district is one of the remote settings in Ethiopia where information about the livestock disease is not well documented. Hence this study was conducted to determine the causative agents and their antimicrobial susceptibility pattern of bacterial Pasteurella isolates among pneumonic ovine in Haramaya district, Eastern Hararghe, Ethiopia.ResultsOut of 256 samples examined, Pasterurella was isolated in 64 (25%), of which 38 (59.4%) were from lungs and 26 (40.6%) were from nasal cavities. 87.5% of the isolates were Mannheimia haemolytica and 12.5% were Pasteurella multocida. All of the isolates from the lungs were Mannheimia haemolytica whereas 69% of the isolates from nasals cavities were Mannheimia haemolytica. Age and body temperature were significantly associated with Pasteurella isolates from clinic (P < 0.05). Despite diverse in the site of origins, the isolates exhibited uniformity in sensitivity to a majority of the antibacterial agents. The most effective drug was Cholramphenicol (100%) followed by Sulfamethoxazole (89.1%) and Tetracycline (84.4%). Both species were completely resistant to Gentamycin and Vancomycin.ConclusionMannheimia haemolytica is the most common cause of ovine pneumonic pasteurellosis in the study area. The isolates were susceptible to limited antimicrobial agents. Therefore, the antimicrobial susceptibility test should be conducted before treatment, except for critical cases.

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Section: Discussionsupporting

confidence: 53%

A study on Ovine pneumonic pasteurellosis: Isolation and Identification of Pasteurellae and their antibiogram susceptibility pattern in Haramaya District, Eastern Hararghe, Ethiopia

2013

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“…Swabs were stored in Cary Blair transport medium (BD Canada, Inc., Mississauga, ON, Canada) at 4°C prior to processing, as described by Klima et al (2011). Briefly, swabs were vortexed in 0.7 mL of brain heart infusion (BHI) broth, and a 100-μL aliquot of the suspension was cultured on tryptic soy agar (TSA) plates containing 5% sheep blood and 15 μg/mL of bacitracin (Dalynn Biologicals, Inc., Calgary, AB, Canada) at 37°C for 24 h (Catry et al, 2006). Isolates displaying morphology indicative of Mannheimia were subcultured (conditions as above) from each plate and tested for catalase and oxidase.…”

Section: Methodsmentioning

confidence: 99%

Susceptibility to tulathromycin in Mannheimia haemolytica isolated from feedlot cattle over a 3-year period

et al. 2013

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Mannheimia haemolytica isolated from feedlot cattle were tested for tulathromycin resistance. Cattle were sampled over a 3-year period, starting 12 months after approval of tulathromycin for prevention and treatment of bovine respiratory disease. Nasopharyngeal samples from approximately 5,814 cattle were collected when cattle entered feedlots (N = 4) and again from the same cattle after ≥60 days on feed. The antimicrobial use history for each animal was recorded. Mannheimia haemolytica was isolated from 796 (13.7%) entry samples and 1,038 (20.6%) ≥ 60 days samples. Of the cattle positive for M. haemolytica, 18.5, 2.9, and 2.4% were administered therapeutic concentrations of tulathromycin, tilmicosin, or tylosin tartrate, respectively. In addition, 13.2% were administered subtherapeutic concentrations of tylosin phosphate in feed. In years one and two, no tulathromycin-resistant M. haemolytica were detected, whereas five isolates (0.4%) were resistant in year three. These resistant isolates were collected from three cattle originating from a single pen, were all serotype 1, and were genetically related (≥89% similarity) according to pulsed-field gel electrophoreses patterns. The five tulathromycin-resistant isolates were multi-drug resistant also exhibiting resistance to oxytetracycline, tilmicosin, ampicillin, or penicillin. The macrolide resistance genes erm(42), erm(A), erm(B), erm(F), erm(X) and msr(E)-mph(E), were not detected in the tulathromycin-resistant M. haemolytica. This study showed that tulathromycin resistance in M. haemolytica from a general population of feedlot cattle in western Canada was low and did not change over a 3-year period after tulathromycin was approved for use in cattle.

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“…Another phenotypic approach to the question of transmission is assessment of antimicrobial resistance. Catry et al found tetracycline-resistant P. multocida in the nasopharynx of 5 calves on 1 farm, while no resistant P. multocida were found in the other 56 calves from 15 farms (Catry et al, 2006). No tetracycline had been used on any farms.…”

Section: Epidemiology Of P Multocida Pneumoniamentioning

confidence: 99%

Pasteurella multocidaand bovine respiratory disease

Dabo

Taylor

Confer

2007

Anim. Health. Res. Rev.

183

153

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Pasteurella multocida is a pathogenic Gram-negative bacterium that has been classified into three subspecies, five capsular serogroups and 16 serotypes. P. multocida serogroup A isolates are bovine nasopharyngeal commensals, bovine pathogens and common isolates from bovine respiratory disease (BRD), both enzootic calf pneumonia of young dairy calves and shipping fever of weaned, stressed beef cattle. P. multocida A:3 is the most common serotype isolated from BRD, and these isolates have limited heterogeneity based on outer membrane protein (OMP) profiles and ribotyping. Development of P. multocida-induced pneumonia is associated with environmental and stress factors such as shipping, co-mingling, and overcrowding as well as concurrent or predisposing viral or bacterial infections. Lung lesions consist of an acute to subacute bronchopneumonia that may or may not have an associated pleuritis. Numerous virulence or potential virulence factors have been described for bovine respiratory isolates including adherence and colonization factors, iron-regulated and acquisition proteins, extracellular enzymes such as neuraminidase, lipopolysaccharide, polysaccharide capsule and a variety of OMPs. Immunity of cattle against respiratory pasteurellosis is poorly understood; however, high serum antibodies to OMPs appear to be important for enhancing resistance to the bacterium. Currently available P. multocida vaccines for use in cattle are predominately traditional bacterins and a live streptomycin-dependent mutant. The field efficacy of these vaccines is not well documented in the literature.

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Detection of Tetracycline-Resistant and Susceptible Pasteurellaceae in the Nasopharynx of Loose Group-Housed Calves

Cited by 28 publications

References 19 publications

A study on Ovine pneumonic pasteurellosis: Isolation and Identification of Pasteurellae and their antibiogram susceptibility pattern in Haramaya District, Eastern Hararghe, Ethiopia

A study on Ovine pneumonic pasteurellosis: Isolation and Identification of Pasteurellae and their antibiogram susceptibility pattern in Haramaya District, Eastern Hararghe, Ethiopia

Susceptibility to tulathromycin in Mannheimia haemolytica isolated from feedlot cattle over a 3-year period

Pasteurella multocidaand bovine respiratory disease

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