Partitioning of xylanase from Thermomyces lanuginosus in PEG/NaCit aqueous two-phase systems: Structural and functional approach

Loureiro, Dana Belén; Braia, Mauricio; Romanini, Diana; Tubío, Gisela

doi:10.1016/j.pep.2016.09.003

Cited by 14 publications

(3 citation statements)

References 18 publications

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“…The conventional multistep purification techniques are time consuming, which increases the cost of the overall process and also results in loss of protein at each step (Iqbal et al 2016;Ramakrishnan et al 2016). The 60-70% of total processing cost in enzyme downstream process comes from the purification step (Loureiro et al 2017;Bhardwaj et al 2019). Therefore, several scientists suggested a single step liquid-liquid fractionation technique, i.e., aqueous two-phase system (Naganagouda and Mulimani 2008;Yasinok et al 2010;Glyk et al 2015).…”

Section: Aqueous Two-phase System Employed For Purification Of Xylanasementioning

confidence: 99%

A detailed overview of xylanases: an emerging biomolecule for current and future prospective

Bhardwaj

Kumar

Verma

2019

Bioresour. Bioprocess.

252

104

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Xylan is the second most abundant naturally occurring renewable polysaccharide available on earth. It is a complex heteropolysaccharide consisting of different monosaccharides such as l-arabinose, d-galactose, d-mannoses and organic acids such as acetic acid, ferulic acid, glucuronic acid interwoven together with help of glycosidic and ester bonds. The breakdown of xylan is restricted due to its heterogeneous nature and it can be overcome by xylanases which are capable of cleaving the heterogeneous β-1,4-glycoside linkage. Xylanases are abundantly present in nature (e.g., molluscs, insects and microorganisms) and several microorganisms such as bacteria, fungi, yeast, and algae are used extensively for its production. Microbial xylanases show varying substrate specificities and biochemical properties which makes it suitable for various applications in industrial and biotechnological sectors. The suitability of xylanases for its application in food and feed, paper and pulp, textile, pharmaceuticals, and lignocellulosic biorefinery has led to an increase in demand of xylanases globally. The present review gives an insight of using microbial xylanases as an "Emerging Green Tool" along with its current status and future prospective.

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Section: Aqueous Two-phase System Employed For Purification Of Xylanasementioning

confidence: 99%

A detailed overview of xylanases: an emerging biomolecule for current and future prospective

Bhardwaj

Kumar

Verma

2019

Bioresour. Bioprocess.

252

104

View full text Add to dashboard Cite

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“…The most common ATPS types are those formed by polymer–polymer or polymer–salt mixtures. ATPS possess several advantages compared to conventional liquid–liquid extraction methods, such as a high-water content, low cost of components, ease of scaling up, process integration capacity, and high yields of the target product (Glyk et al 2015 ; Gómez and Macedo 2019 ; Loureiro et al 2017 ). When a solute is added into the ATPS, the partition between the two phases responds to several variables, namely the type and concentration of phase-forming components, molecular weight of polymers, system pH, temperature, and intrinsic physicochemical properties of the solute (Asenjo and Andrews 2011 ; Gu and Glatz 2007 ; Iqbal et al 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Primary recovery of hyaluronic acid produced in Streptococcus equi subsp. zooepidemicus using PEG–citrate aqueous two-phase systems

et al. 2021

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Given its biocompatibility, rheological, and physiological properties, hyaluronic acid (HA) has become a biomaterial of increasing interest with multiple applications in medicine and cosmetics. In recent decades, microbial fermentations have become an important source for the industrial production of HA. However, due to its final applications, microbial HA must undergo critical and long purification processes to ensure clinical and cosmetic grade purity. Aqueous two-phase systems (ATPS) have proven to be an efficient technique for the primary recovery of high-value biomolecules. Nevertheless, their implementation in HA downstream processing has been practically unexplored. In this work, polyethylene glycol (PEG)–citrate ATPS were used for the first time for the primary recovery of HA produced with an engineered strain of Streptococcus equi subsp. zooepidemicus. The effects of PEG molecular weight (MW), tie-line length (TLL), volume ratio (VR), and sample load on HA recovery and purity were studied with a clarified fermentation broth as feed material. HA was recovered in the salt-rich bottom phase, and its recovery increased when a PEG MW of 8000 g mol−1 was used. Lower VR values (0.38) favoured HA recovery, whereas purity was enhanced by a high VR (3.50). Meanwhile, sample load had a negative impact on both recovery and purity. The ATPS with the best performance was PEG 8000 g mol−1, TLL 43% (w/w), and VR 3.50, showing 79.4% HA recovery and 74.5% purity. This study demonstrated for the first time the potential of PEG–citrate ATPS as an effective primary recovery strategy for the downstream process of microbial HA.

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“…[9,12,13] This extraction system has several advantages over conventional extraction procedures such as solvent extraction, including the absence of poisonous and dangerous ingredients, a quick phase separation time, [14] and low viscosity. [15][16][17] Metals [18,19] and biological compounds such as proteins, [20] enzymes, [21] and antibodies [22,23] can all be isolated and extracted using the aqueous two-phase system. Polymerpolymer, [24] polymer-salt, [25][26][27] ionic liquid-salt, [28] and alcohol-salt [8,29] are examples of aqueous two-phase systems.…”

Section: Introductionmentioning

confidence: 99%

Optimizing the extraction of cobalt ions under response surface methodology and without organic solutions

et al. 2022

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In this study, response surface methodology was used to evaluate the extraction of cobalt ions using an aqueous two‐phase system (ATPS). This method is environmentally benign and low‐risk because water has replaced the poisonous and dangerous organic solvents in this extraction procedure. For the first time, a central composite design was employed to examine the effect of various factors on the extraction of cobalt ions. The findings of the experimental design revealed that the concentration and volume of cobalt ions had a considerable impact on the extraction percentage in the ATPS system. In contrast, the concentration of ammonium sulphate and pH had only a minor effect. The value of the correlation coefficient (R2 = 0.9565) clearly shows the agreement between the mathematical model and the experimental results. The maximum extraction percentage was attained in optimum conditions (50% polyethylene glycol concentration, 3.75 M salt concentration, 0.5 ml cobalt volume, and pH 4).

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Partitioning of xylanase from Thermomyces lanuginosus in PEG/NaCit aqueous two-phase systems: Structural and functional approach

Cited by 14 publications

References 18 publications

A detailed overview of xylanases: an emerging biomolecule for current and future prospective

A detailed overview of xylanases: an emerging biomolecule for current and future prospective

Primary recovery of hyaluronic acid produced in Streptococcus equi subsp. zooepidemicus using PEG–citrate aqueous two-phase systems

Optimizing the extraction of cobalt ions under response surface methodology and without organic solutions

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