Particle size investigations of a multistep synthesis of PVA coated superparamagnetic nanoparticles

Chastellain, M.; Petri, A.; Hofmann, Heinrich

doi:10.1016/j.jcis.2004.06.025

Cited by 178 publications

(131 citation statements)

References 38 publications

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“…This is similar to other reports in the Refs. [13,20]. The PAMNs were nearly spherical in shape and well-separated, but there was no significant change in size resulting from the PA coating process, as shown in Fig.…”

Section: Preparation and Characterization Of Pamnsmentioning

confidence: 88%

“…Because of their hydrophobic surfaces and large surface area to volume ratio, in vivo use of nano-magnetic particles trends to result in agglomeration and very rapid clearance from the circulation. In addition, a common problem of uncoated magnetic nanoparticles is their chemical instability with respect to oxidation in air [13]. In the case of iron oxides, the final compositions are very often intermediate between magnetite (Fe 3 O 4 ) and maghemite (γ-Fe 2 O 3 ), due to oxidation of the particles during the synthesis [14].…”

Section: Introductionmentioning

confidence: 99%

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Pullulan acetate coated magnetite nanoparticles for hyper-thermia: Preparation, characterization and in vitro experiments

et al. 2010

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Amphipathic polymer pullulan acetate (PA)-coated magnetic nanoparticles were prepared and characterized by various physicochemical means. The cytotoxicity and cellular uptake of the magnetic nanoparticles were examined. The hyperthermic effect of the magnetic nanoparticles on tumor cells was evaluated. Transmission electron microscopy (TEM) showed that the PA coated magnetic nanoparticles (PAMNs) had spherical morphology. Dynamic light scattering (DLS) showed that the size distribution of PAMNs was unimodal,with an average diameter of 25.8 nm ± 6.1 nm. The presence of the adsorbed layer of PA on the magnetite surface was confirmed by Fourier transform infrared (FTIR) spectroscopy. Magnetic measurements revealed that the saturation magnetization of the PAMNs reached 51.9 emu/g and the nanoparticles were superparamagnetic. Thermogravimetric analysis (TGA) showed that the Fe 3 O 4 particles constituted 75 wt% of the PAMNs. The PAMNs had good heating properties in an alternating magnetic field. Cytotoxicity assay showed that PAMNs exhibited no significant cytotoxicity against L929 cells. TEM results showed that a large number of PAMNs were internalized into KB cells. PAMNs have good hyperthermia effect on KB cells in vitro by magnetic field induced hyperthermia. These novel magnetic nanoparticles have great potential as magnetic hyperthermia mediators.

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Section: Preparation and Characterization Of Pamnsmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Pullulan acetate coated magnetite nanoparticles for hyper-thermia: Preparation, characterization and in vitro experiments

et al. 2010

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“…SPIONs were synthesized by the alkaline co-precipitation of ferric and ferrous chlorides in aqueous solution 21 following the work reported by Bee 22 and van Ewijk 23 . Surface modification of SPIONs with APTES was accomplished adapting the procedure of Yamaura et al 24 .…”

Section: Synthesis and Surface Modification Of Spionsmentioning

confidence: 99%

Magnetic microreactors for efficient and reliable magnetic nanoparticle surface functionalization

et al. 2014

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Microreactors have attracted wide attention in the nano-and biotechnology field because they offer many advantages over standard liquid phase reactions. We report the development of a magnetic microreactor for reliable, fast and efficient surface functionalization of superparamagnetic iron oxide nanoparticles (SPIONs). A comprehensive study is described of the development process in terms of setup, loading capacity and efficiency. We have performed experimental and computational studies in order to evaluate the trapping efficiencies, maximum loading capacity and magnetic alignment of the nanoparticles. The results show that capacity and trapping efficiencies are directly related to the flow rate, elution time and reactor type. Based on our results and the developed magnetic microreactor, we describe a model multistep surface derivatization procedure of SPIONs.

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“…Briefly, SPIONs were prepared by alkaline co-precipitation of ferric and ferrous chlorides in aqueous solution (Bee et al 1995;Chastellain et al 2004). The obtained precipitate was washed several times with ultrapure water and the remaining solid was refluxed in nitric acid (10 M) in the presence of Iron-(III)-nitrate (FLUKA).…”

Section: Superparamagnetic Iron Oxide Nanoparticles (Spions)mentioning

confidence: 99%

Biomedical nanoparticles modulate specific CD4⁺T cell stimulation by inhibition of antigen processing in dendritic cells

et al. 2011

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Understanding how nanoparticles may affect immune responses is an essential prerequisite to developing novel clinical applications. To investigate nanoparticle-dependent outcomes on immune responses, dendritic cells (DCs) were treated with model biomedical poly(vinylalcohol)-coated super-paramagnetic iron oxide nanoparticles (PVA-SPIONs). PVA-SPIONs uptake by human monocyte-derived DCs (MDDCs) was analyzed by flow cytometry (FACS) and advanced imaging techniques. Viability, activation, function, and stimulatory capacity of MDDCs were assessed by FACS and an in vitro CD4 + T cell assay. PVA-SPION uptake was dose-dependent, decreased by lipopolysaccharide (LPS)-induced MDDC maturation at higher particle concentrations, and was inhibited by cytochalasin D pre-treatment. PVA-SPIONs did not alter surface marker expression (CD80, CD83, CD86, myeloid/plasmacytoid DC markers) or antigen-uptake, but decreased the capacity of MDDCs to process antigen, stimulate CD4 + T cells, and induce cytokines. The decreased antigen processing and CD4 + T cell stimulation capability of MDDCs following PVA-SPION treatment suggests that MDDCs may revert to a more functionally immature state following particle exposure.

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Particle size investigations of a multistep synthesis of PVA coated superparamagnetic nanoparticles

Cited by 178 publications

References 38 publications

Pullulan acetate coated magnetite nanoparticles for hyper-thermia: Preparation, characterization and in vitro experiments

Pullulan acetate coated magnetite nanoparticles for hyper-thermia: Preparation, characterization and in vitro experiments

Magnetic microreactors for efficient and reliable magnetic nanoparticle surface functionalization

Biomedical nanoparticles modulate specific CD4⁺T cell stimulation by inhibition of antigen processing in dendritic cells

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Particle size investigations of a multistep synthesis of PVA coated superparamagnetic nanoparticles

Cited by 178 publications

References 38 publications

Pullulan acetate coated magnetite nanoparticles for hyper-thermia: Preparation, characterization and in vitro experiments

Pullulan acetate coated magnetite nanoparticles for hyper-thermia: Preparation, characterization and in vitro experiments

Magnetic microreactors for efficient and reliable magnetic nanoparticle surface functionalization

Biomedical nanoparticles modulate specific CD4+T cell stimulation by inhibition of antigen processing in dendritic cells

Contact Info

Product

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Biomedical nanoparticles modulate specific CD4⁺T cell stimulation by inhibition of antigen processing in dendritic cells