Magnetic microreactors for efficient and reliable magnetic nanoparticle surface functionalization

Abstract

Microreactors have attracted wide attention in the nano-and biotechnology field because they offer many advantages over standard liquid phase reactions. We report the development of a magnetic microreactor for reliable, fast and efficient surface functionalization of superparamagnetic iron oxide nanoparticles (SPIONs). A comprehensive study is described of the development process in terms of setup, loading capacity and efficiency. We have performed experimental and computational studies in order to evaluate the trapping efficiencies, maximum loading capacity and magnetic alignment of the nanoparticles. The results show that capacity and trapping efficiencies are directly related to the flow rate, elution time and reactor type. Based on our results and the developed magnetic microreactor, we describe a model multistep surface derivatization procedure of SPIONs.

Uptake and Intracellular Fate of Peptide Surface-Functionalized Silica Hybrid Magnetic Nanoparticles In Vitro

“…As a control, dye‐labeled analogs using AlexaFluor488‐peptide or SAMSA‐fluorescein‐PEG have been synthesized to further demonstrate the presence of the polymer and peptides, respectively, and to obtain a rough estimate about their quantity. The fluorescence spectra show an emission at 515 nm, which is characteristic for the SAMSA‐fluorescein dye . A PEG grafting density of approximately 200 PEG molecules per NP can be estimated, corresponding to a coverage density of 0.01 PEG chains per nm 2 , which is consistent with previous studies .…”

Magnetic Microreactors with Immobilized Enzymes—From Assemblage to Contemporary Applications

“…Moreover, greater control over reaction conditions and rapid temperature changes are possible, leading to greater yields of products and higher purities. Small volumes required and facile heat exchange and mass transfer are also crucial features that distinguish microsystems from conventional apparatus [20,21].…”

Plasmonic nanoparticles and their characterization in physiological fluids

“…One approach to characterize aggregation and the proteins adsorbed onto the surface of magnetic NPs is to separate the NPprotein complex after injection into the blood stream of rats by a high magnetic field gradient magnetic reactor [35]. It was reported that the protein composition depends on the initial surface charge of different functionalized NPs [36].…”