Uptake and Intracellular Fate of Peptide Surface-Functionalized Silica Hybrid Magnetic Nanoparticles In Vitro

Digigow, Reinaldo; Vanhecke, Dimitri; Rothen‐Rutishauser, Barbara; Clift, Martin J. D.; Petri‐Fink, Alke

doi:10.1002/ppsc.201400152

Cited by 4 publications

(3 citation statements)

References 53 publications

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“…The here described multifunctional NPs have been used in an in-depth microscopy study, where we use laser scanning (LSM), transmission electron (TEM) and dark field microscopy to investigate NP internalization and fate with macrophage (J774A.1) and epithelial (HeLa) cells in much detail. 48 …”

Section: Discussionmentioning

confidence: 99%

Magnetic microreactors for efficient and reliable magnetic nanoparticle surface functionalization

et al. 2014

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Microreactors have attracted wide attention in the nano-and biotechnology field because they offer many advantages over standard liquid phase reactions. We report the development of a magnetic microreactor for reliable, fast and efficient surface functionalization of superparamagnetic iron oxide nanoparticles (SPIONs). A comprehensive study is described of the development process in terms of setup, loading capacity and efficiency. We have performed experimental and computational studies in order to evaluate the trapping efficiencies, maximum loading capacity and magnetic alignment of the nanoparticles. The results show that capacity and trapping efficiencies are directly related to the flow rate, elution time and reactor type. Based on our results and the developed magnetic microreactor, we describe a model multistep surface derivatization procedure of SPIONs.

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Section: Discussionmentioning

confidence: 99%

Magnetic microreactors for efficient and reliable magnetic nanoparticle surface functionalization

et al. 2014

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“…There are several cellular candidates in the circulation which may act as NP Trojan horses such as red blood cells, monocytes/macrophages, T cells, B cells, or dendritic cells because of their proximity to circulation . In this study, we focused on monocytes/macrophages as a model cell type because they phagocytose nanomaterials and are mobile within tissue . Macrophages serve several physiological functions such as wound healing, host defense, and immune regulation.…”

Section: Introductionmentioning

confidence: 99%

“…15 Macrophages serve several physiological functions such as wound healing, host defense, and immune regulation. Monocytes, which originate from the hematopoietic stem cell line, circulate in the bloodstream and can be signaled to cross endothelial barriers via luminal surface markers such as intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1.…”

Section: ■ Introductionmentioning

confidence: 99%

Cellular Shuttles: Monocytes/Macrophages Exhibit Transendothelial Transport of Nanoparticles under Physiological Flow

Moore¹,

Hauser²,

Gruber

et al. 2017

ACS Appl. Mater. Interfaces

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A major hurdle in the development of biomedical nanoparticles (NP) is understanding how they interact with complex biological systems and navigate biological barriers to arrive at pathological targets. It is becoming increasingly evident that merely controlling particle physicochemical properties may not be sufficient to mediate particle biodistribution in dynamic environments. Thus, researchers are increasingly turning toward more complex but likewise more physiological in vitro systems to study particle−-cell/particle−system interactions. An emerging paradigm is to utilize naturally migratory cells to act as so-called "Trojan horses" or cellular shuttles. We report here the use of monocytes/macrophages to transport NP across a confluent endothelial cell layer using a microfluidic in vitro model. With a custom-built flow chamber, we showed that physiological shear stress, when compared to low flow or static conditions, increased NP uptake by macrophages. We further provided a mathematical explanation for the effect of flow on NP uptake, namely that the physical exposure times of NP to cells is dictated by shear stress (i.e., flow rate) and results in increased particle uptake under flow. This study was extended to a multicellular, hydrodynamic in vitro model. Because monocytes are cells that naturally translocate across biological barriers, we utilized a monocyte/macrophage cell line as cellular NP transporters across an endothelial layer. In this exploratory study, we showed that monocyte/macrophage cells adhere to an endothelial layer and dynamically interact with the endothelial cells. The monocytes/macrophages took up NP and diapedesed across the endothelial layer with NP accumulating within the cellular uropod. These data illustrate that monocytes/macrophages may therefore act as active shuttles to deliver particles across endothelial barriers.

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The timeline of corona formation around silica nanocarriers highlights the role of the protein interactome

et al. 2017

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Magnetic mesoporous silica nanoparticles (M-MSNs) represent promising targeting tools for theranostics. Engineering the interaction of nanoparticles (NPs) with biological systems requires an understanding of protein corona formation around the nanoparticles as this drives the biological fate of nanocarriers. We investigated the behavior of proteins in contact with M-MSNs by high-throughput comparative proteomics, using human and bovine sera as biological fluids, in order to assess the adsorption dynamics of proteins in these media. Using system biology tools, and especially protein-protein interaction databases, we demonstrated how the protein network builds up within the corona over the course of the experiment. Based on these results, we introduce and discuss the role of the "corona interactome" as an important factor influencing protein corona evolution. The concept of the "corona interactome" is an original methodology which could be generalized to all NP candidates. Based on this, pre-coating nanocarriers with specific proteins presenting minimal interactions with opsonins might provide them with properties such as stealth.

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Uptake and Intracellular Fate of Peptide Surface-Functionalized Silica Hybrid Magnetic Nanoparticles In Vitro

Cited by 4 publications

References 53 publications

Magnetic microreactors for efficient and reliable magnetic nanoparticle surface functionalization

Magnetic microreactors for efficient and reliable magnetic nanoparticle surface functionalization

Cellular Shuttles: Monocytes/Macrophages Exhibit Transendothelial Transport of Nanoparticles under Physiological Flow

The timeline of corona formation around silica nanocarriers highlights the role of the protein interactome

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