Participation of excitatory amino acid receptors in the slow excitatory synaptic transmission in rat spinal dorsal horn

Gerber, Gábor; Cerne, R.; Randić, Mirjana

doi:10.1016/0006-8993(91)91600-6

Cited by 55 publications

(20 citation statements)

References 75 publications

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“…Excitatory amino acid receptor ligands excite different groups of spinal cord neurons (Jahr and Jessell, 1985;Evans et al, 1987;Davies et al, 1988;King et al, 1988;Schneider and Perl, 1988;Jeftinija, 1989;Yoshimura and Jessell, 1990;Gerber and Randic, 1991). One view has been that AMPA and kainate receptors play a role in the generation of fast EPSPs at the first synapse between primary afferents and secondary sensory neurons (Schneider and Perl, 1988;Yoshimura and Jessell, 1990) or motoneurons (Buhrle and Sonnhof, 1983;Jahr and Yoshioka, 1986), while NMDA receptors are involved in the excitatory transmission through polysynaptic pathway in the dorsal horn (Watkins and Evans, 1981;Jessell et al, 1986;Headley and Grillner, 1991).…”

Section: Lamina IImentioning

confidence: 99%

Cobalt accumulation in neurons expressing ionotropic excitatory amino acid receptors in young rat spinal cord: Morphology and distribution

Nagy

Woolf

Dray³

et al. 1994

J of Comparative Neurology

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Excitatory amino acids (EAA) acting on N-methyl-D-aspartate (NMDA), alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and kainate receptors play an important role in synaptic transmission in the spinal cord. Quantitative autoradiography and physiological experiments suggest that NMDA receptors are localized mainly in lamina II while kainate and AMPA receptors are found on both dorsal and ventral horn neurons. However the cell types expressing EAA receptors and their laminar distribution is not known. We have used a cobalt uptake method to study the morphology and distribution of spinal cord neurons expressing AMPA, kainate, or NMDA excitatory amino acid receptors in the lumbar enlargement of the rat spinal cord. The technique involved superfusion of hemisected spinal cords of 14 day-old rat pups in vitro with excitatory amino acid receptor ligands in the presence of CoCl2. Cobalt has been shown to enter cells through ligand-gated ion channels in place of Ca2+. Cells which accumulated cobalt ions following activation by ionotropic excitatory amino acid receptors were visualized histochemically. The cobalt uptake generated receptor-specific labeling of cells, as the NMDA receptor antagonist D-(-)-2-amino-(5)-phosphonovaleric acid (D-AP-5) (20 microM) blocked the NMDA, but not kainate-induced cobalt uptake. The kainate-induced cobalt labeling was reduced by the non-selective excitatory amino acid receptor antagonist kynurenic acid (4 mM). Passive opening of the voltage-gated Ca(2+)-channels by KCl (50 mM) did not result in cobalt uptake, indicating that cobalt enters the cells through ligand-gated Ca(2+)-channels. AMPA (500 microM), kainate (500 microM), or NMDA (500 microM) each induced cobalt uptake with characteristic patterns and distributions of neuronal staining. Overall, kainate induced cobalt uptake in the greatest number of neuronal staining. Overall, kainate induced cobalt uptake in the greatest number of neuronal perikarya while NMDA-induced uptake was the lowest. AMPA and kainate, but not NMDA superfusion, resulted in cobalt labeling of glial cells. Our results show that the cobalt uptake technique is a useful way to study the morphology and distribution of cells expressing receptors with ligand-gated Ca2+ channels.

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Section: Lamina IImentioning

confidence: 99%

Cobalt accumulation in neurons expressing ionotropic excitatory amino acid receptors in young rat spinal cord: Morphology and distribution

Nagy

Woolf

Dray³

et al. 1994

J of Comparative Neurology

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“…It appears likely that both glutamate and neuropeptides are released following activation of terminals containing both these substances. While glutamate rapidly excites dorsal horn neurons, peptides are presumably involved in more long-term effects on the response of dorsal horn neurons (although EAAs have been implicated in slow excitatory neurotransmission as well ;Gerber et al, 1991).…”

Section: Glutamate As a Putative Primary Afferent Neurotransmittermentioning

confidence: 99%

“…Recent experiments Perl, 1985, 1988) have indicated that only some dorsal horn neurons respond to exogeneously applied glutamate, and that such neurons occur mainly in the superficial part of the dorsal horn, thus supporting a role for glutamate as a transmitter primarily in primary afferent fibres that convey nociceptive information. However, the results of other investigations suggest that glutamate is a neurotransmitter also of primary afferent fibres that terminate in the deeper parts of the dorsal horn (Schouenborg and Sjolund, 1986;King et al, 1988; Morris, 1989;Gerber and Randic, 1989;Gerber et al, 1991;Dougherty et al, 1992).…”

Section: Introductionmentioning

confidence: 98%

Enrichment of Glutamate‐like Immunoreactivity in Primary Afferent Terminals Throughout the Spinal Cord Dorsal Horn

Broman

Anderson

Ottersen

1993

Eur J of Neuroscience

106

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Although several lines of evidence indicate that glutamate is a neurotransmitter in primary afferent terminals, controversies exist on the proportion and types of such terminals that release glutamate. In the present study quantitative analysis of immunogold labelling was used to assess the presence of glutamate-like immunoreactivity in primary afferent terminals in laminae I-V of the rat spinal cord dorsal horn. Anterograde transport of choleragenoid-horseradish peroxidase from a spinal ganglion and tetramethyl benzidine histochemistry were used to identify primary afferent terminals in laminae I and III-V. Presumed C-fibre terminals in lamina II were identified on morphological criteria (dense sinusoid axon terminals). Primary afferent terminals in all dorsal horn laminae displayed significantly higher levels of glutamate-like immunoreactivity than pleomorphic vesicle-containing profiles in laminae III-IV and large neuronal cell bodies in laminae III-V. The density of gold particles over primary afferent terminals also significantly exceeded the average density of gold particles over laminae II and III-IV. The highest densities of gold particles were present over dense sinusoid axon terminals in lamina II. These findings suggest that glutamate, alone or in combination with other neuroactive compounds, is involved in the transfer of all sensory modalities from primary afferent fibres to dorsal horn neurons.

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“…Glutamatergic influence is exerted through ionotropic and metabotropic receptor classes, but those that usually guarantee fast responses (Watkins et al, 1990;Gerber et al, 1991) controlling neural reflexes, are typically performed by ion channel receptor-type (Currie and Stein, 1992;Smith and Darlington, 1996;Priesol et al, 2000). Based on their pharmacologic properties, glutamate ionotropic family receptors include NMDA (N-methyl-D-aspartate) and two non-NMDA subtypes: a-amino-3-hidroxy-5-methyl-4-isoxalone propionate (AMPA) type and kainate receptor-type (Foster and Fagg, 1984;Ozawa et al, 1998;Chen et al, 2000;Chan et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Morphometric analysis of the AMPA-type neurons in the Deiters vestibular complex of the chick brain

Passetto

Britto

Toledo

2008

Journal of Chemical Neuroanatomy

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Participation of excitatory amino acid receptors in the slow excitatory synaptic transmission in rat spinal dorsal horn

Cited by 55 publications

References 75 publications

Cobalt accumulation in neurons expressing ionotropic excitatory amino acid receptors in young rat spinal cord: Morphology and distribution

Cobalt accumulation in neurons expressing ionotropic excitatory amino acid receptors in young rat spinal cord: Morphology and distribution

Enrichment of Glutamate‐like Immunoreactivity in Primary Afferent Terminals Throughout the Spinal Cord Dorsal Horn

Morphometric analysis of the AMPA-type neurons in the Deiters vestibular complex of the chick brain

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