Morphometric analysis of the AMPA-type neurons in the Deiters vestibular complex of the chick brain

Passetto, Maria F.; Britto, Luiz Roberto Giorgetti de; Toledo, Cláudio Fabiano Motta

doi:10.1016/j.jchemneu.2008.02.003

Cited by 7 publications

(16 citation statements)

References 92 publications

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“…Our high resolution electroporation-based labelling allowed for a detailed characterisation of the axial origin of vestibular neuronal types (Figure 8). The Nucleus Deiters Ventralis (Dv) and Nucleus Deiters Dorsalis (Dd) of birds are homologous to the rostroventral and dorsocaudal part of the mammalian lateral vestibular nucleus, respectively (Passetto et al, 2008), which is mainly derived from r4 progenitors (Chen et al, 2012;Di Bonito et al, 2015), with additional contribution from r3 and r5 (Pasqualetti et al, 2007). Here, we observed a similar axial origin for both the Dv and Dd, with progenitors allocated to r3, r4 and r5 (Figures 6 I,J and 9).…”

Section: Anatomical Morphological and Developmental Features Indicatsupporting

confidence: 61%

Conserved and divergent development of brainstem vestibuloacoustic nuclei

Lipovsek

Wingate

2018

Preprint

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Vestibular function was established early in vertebrates and has remained, for the most part, unchanged. In contrast, tetrapods underwent independent evolutionary processes to solve the problem of hearing on land. Thus, the vestibuloacoustic nuclei of the hindbrain provide an ideal framework on which to address the participation of developmental processes to the evolution of neuronal circuits.We employed an electroporation strategy to unravel the contribution of dorsoventral and axial lineages to the development of the chick hindbrain vestibular and auditory nuclei. We compare the chick developmental map with recently stablished genetic fate-maps of the mouse hindbrain.Overall, we find considerable conservation of developmental origin for the vestibular nuclei. In contrast, auditory hindbrain development echoes the complex evolutionary history of the auditory system. In particular, we find that the developmental origin of a chick sound localisation circuit supports its emergence from an ancient vestibular network, unrelated to the analogous mammalian counterpart.

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Section: Anatomical Morphological and Developmental Features Indicatsupporting

confidence: 61%

Conserved and divergent development of brainstem vestibuloacoustic nuclei

Lipovsek

Wingate

2018

Preprint

View full text Add to dashboard Cite

show abstract

“…Our high resolution electroporation-based labelling allowed for a detailed characterisation of the axial origin of vestibular neuronal types (Figure 8). The Nucleus Deiters Ventralis (Dv) and Nucleus Deiters Dorsalis (Dd) of birds are homologous to the rostroventral and dorsocaudal part of the mammalian lateral vestibular nucleus, respectively (Passetto et al, 2008), which is mainly derived from r4 progenitors (Chen et al, 2012; Di Bonito et al, 2015), with additional contribution from r3 and r5 (Pasqualetti et al, 2007). Here, we observed a similar axial origin for both the Dv and Dd, with progenitors allocated to r3, r4 and r5 (Figures 4I,J and 8).…”

Section: Discussionmentioning

confidence: 99%

Conserved and divergent development of brainstem vestibular and auditory nuclei

Lipovsek

Wingate

2018

eLife

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Vestibular function was established early in vertebrates and has remained, for the most part, unchanged. In contrast, each group of tetrapods underwent independent evolutionary processes to solve the problem of hearing on land, resulting in a remarkable mixture of conserved, divergent and convergent features that define extant auditory systems. The vestibuloacoustic nuclei of the hindbrain develop from a highly conserved ground plan and provide an ideal framework on which to address the participation of developmental processes to the evolution of neuronal circuits. We employed an electroporation strategy to unravel the contribution of two dorsoventral and four axial lineages to the development of the chick hindbrain vestibular and auditory nuclei. We compare the chick developmental map with recently established genetic fate-maps of the developing mouse hindbrain. Overall, we find considerable conservation of developmental origin for the vestibular nuclei. In contrast, a comparative analysis of the developmental origin of hindbrain auditory structures echoes the complex evolutionary history of the auditory system. In particular, we find that the developmental origin of the chick auditory interaural time difference circuit supports its emergence from an ancient vestibular network, unrelated to the analogous mammalian counterpart.

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“…The images were captured by selecting brain regions with a 10× ocular of a Nikon Eclipse E 800 (Nikon Corporation, Chiyoda-Ku, Tokyo-To, Japan), a microscope equipped with a CCD camera (Optronics MagnaFire, Goleta, CA, USA) connected to a Macintosh desktop computer, and figures were prepared using the Adobe Photoshop software. No attempt was made to quantify the labeled structures or to evaluate the intensity of the markers, but when needed, we estimated the cellular size by measuring along the larger neuronal axis as priory described 16 . Some general controls for the specificity of the AMPA immuno-staining were previously reported [24][25][26][27] , but to validate our own results incubation without the primary antibodies was performed.…”

Section: Methodsmentioning

confidence: 99%

“…1). The VNC neurons are rich in AMPA-type glutamic acid receptors [16][17][18] , supposedly targeted by the glutamic acid of Scarpa's ganglion neurons, but the characteristics of these connections are missing. While many pharmacological properties are familiar 12,13,[18][19][20] the morphological details of its circuitry remain unknown.…”

Section: Introductionmentioning

confidence: 99%

Pigeon as a model to study peripheral projections from the horizontal semicircular canal vestibular apparatus to a brainstem target immunoreactive for AMPA

et al. 2021

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Purpose: To evaluate whether the pigeon (Columba livia) is a good model for evaluating the vestibular system involved with postural maintenance during movement. Methods: This study maps the brainstem targets of the horizontal ampullary inputs from the vestibular periphery of the pigeon. We used biotin dextran amine (BDA) injection in horizontal semicircular canal (HSCC), immunohistochemistry for GluR2/3 and GluR4 AMPA and computerized histomorphology reconstruction. Results: Our results show the same distribution pattern with ipsilateral projections to vestibular nuclear complex (VNC) from the HSCC, with the majority of labeled fibers being, long, thin, with few varicosities and many ramifications. Horizontal semicircular canal projections achieve neurons belonging to all nuclei of the VNC with exception of dorsal portion of lateral vestibular nucleus and this area express GluR2/3 and GluR4 AMPA receptors reinforcing the idea of glutamate participation in these connections. Conclusions: Pigeon is an appropriated experimental model to study of projections of HSCC and reinforcing the information that the vestibular system has strong relation with the fast responses necessary for postural control. Moreover, its phylogenetic organization apparently conservation, also seems to be a fundamental characteristic for vertebrates.

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Morphometric analysis of the AMPA-type neurons in the Deiters vestibular complex of the chick brain

Cited by 7 publications

References 92 publications

Conserved and divergent development of brainstem vestibuloacoustic nuclei

Conserved and divergent development of brainstem vestibuloacoustic nuclei

Conserved and divergent development of brainstem vestibular and auditory nuclei

Pigeon as a model to study peripheral projections from the horizontal semicircular canal vestibular apparatus to a brainstem target immunoreactive for AMPA

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