Partial purification of a Bacillus licheniformis levansucrase producing levan with antitumor activity

Dahech, Imen; Belghith, Karima; Belghith, Hafedh; Mejdoub, Hafedh

doi:10.1016/j.ijbiomac.2012.04.030

Cited by 43 publications

(27 citation statements)

References 30 publications

(28 reference statements)

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“…Levan, a fructan with β ‐(2‐6) glycosidic linkages and some β ‐(1‐2)‐branching, also has applications in food and pharmaceutical industries. It can act as a stabilizer, encapsulating agent, is used in bio‐films and has shown anti‐tumour activities . With its low caloric value, levan‐type FOSs and levan can also be used in the formulation of low‐calorie foods …”

Section: Introductionmentioning

confidence: 99%

Immobilization and stabilization of levansucrase biocatalyst of high interest for the production of fructooligosaccharides and levan

Hill

Karboune

Mateo

2015

J of Chemical Tech & Biotech

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BACKGROUND: Levansucrase (LS)-catalyzed-transfructosylation reaction is a potential approach for the synthesis of fructooligosaccharides (FOSs) and levan as health promoting compounds. This biocatalytic approach is hindered by low thermal stability of LS and its high rate of hydrolysis. In the present study, LS from Bacillus amyloliquefaciens was immobilized onto modified and unmodified epoxy-activated supports (Eupergit ® C; Sepabeads ® ) and on modified cross-linked-agarose beads, to increase its thermal stability and modulate its reaction selectivity (hydrolysis/transfructosylation).

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Section: Introductionmentioning

confidence: 99%

Immobilization and stabilization of levansucrase biocatalyst of high interest for the production of fructooligosaccharides and levan

Hill

Karboune

Mateo

2015

J of Chemical Tech & Biotech

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“…. licheniformis reported by Dahech et al catalyzes the formation of fructo-oligosaccharides and the synthesis of Levan polymers, a larger group of commercially important polymers which is a source of prebiotic bre(Dahech et al, 2012;Franken, Brandt, Tai, & Bauer, 2013;GMT, Lima, de Franca, & Lopes, 2000;Kim et al, 2005;Yoo, Yoon, Cha, & Lee, 2004). Levan showed in vitro cytotoxicity against HepG2 cells (highest at 200 mg/ ml) when applied in a dose-dependent manner whereas no e ect was observed against WRL68 (Human fetal liver) cells(Dahech et al, 2012).…”

mentioning

confidence: 99%

Bacillus spp.: Attractive Sources of Anti-cancer and Anti-proliferative Biomolecules

Ferdous¹,

Shishir²,

Khan³

et al. 2018

Microb. Bioacts.

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Cancer treatment remains as an expensive process due to the cost of sophisticated infrastructure development as well as its maintenance with skilled personnel. At the same time, the success rate is not very inspiring since non-specific target oriented medication could cause other health complexities leading to death. Research for alternative therapies aimed at minimizing the side effects of treatments and increasing the survival rates of patients includes routine explorations for anticancer agents from numerous sources (e.g. microbes, plants and nanoparticles). Anticancer activities of several bacterial components especially from Bacillus spp. were reported in many scientific reports. For economic production of these agents, potential strains from this genus could be feasible and sustainable for their long and successful utilization in industries. The review is therefore, focused on describing the available anticancer and anti-proliferative agents reported worldwide from Bacillus spp.

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“…The dried levan was quantified by weighing the dried precipitate (Han ; Dahech et al . ). Purity and molecular weight of the levan were determined by the above‐mentioned methods.…”

Section: Methodsmentioning

confidence: 97%

“…The sediment obtained from the 200-mL culture liquid was incubated with 100 mL of 600 g L À1 sucrose at 45°C for 48 h. Levan was purified from the incubation solution by repeated ethanol precipitation, dissolved in distilled water and dried using freeze drier (EYELA Freeze Dryer FDU-2200; Tokyo Rikakikai, Tokyo, Japan). The dried levan was quantified by weighing the dried precipitate (Han 1990;Dahech et al 2012). Purity and molecular weight of the levan were determined by the abovementioned methods.…”

Section: Mass Levan Production By B Licheniformis Fri My-55mentioning

confidence: 99%

“…Microbial levans are produced from sucrose‐based substrates via transfructosylation of levansucrase (beta‐2, 6 fructan: D‐glucose‐fructosyl transferase, EC 2.4.1.10) by microorganisms, such as Aerobacter aerogenes , Bacillus subtilis , Erwinia herbicola and Zymomonas mobilis (Han ; Keith, Wiley, Ball, Arcidiacono, Zorfass & Mayer ). Levans have industrial importance and applications in the cosmetic, pharmaceutical and food industries (Han ; Calazans, Lopes, Lima & de França ; Yoon, Yoo, Cha & Lee ; No, Park, Kim, Jo, Lee & Ly ; Belghith, Dahech, Hamden, Feki, Mejdoub & Belghith ; Dahech, Belghith, Belghith & Mejdoub ). They also have prebiotic properties (Dal Bello, Walter, Hertel & Hammes ; Korakli, Gänzle & Vogel ; Semjonovs & Zikmanis ; Huang, Lee, Ho, Lin & Pan ) and can influence the immune system (Calazans, Lima, de França & Lopes ; Yoo, Yoon, Cha & Lee ; Liu, Lu, Lu, Liu, Wang & Xiao ).…”

Section: Introductionmentioning

confidence: 99%

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Effects of dietary levan on growth performance, nonspecific immunity, pathogen resistance and body composition of orange-spotted grouper (Epinephelus coioidesH.)

Huang

Chang

et al. 2014

Aquac Res

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This study investigated the effects of levan produced by Bacillus licheniformis FRI MY‐55 on growth performance, intestinal count of viable bacteria, immune status, pathogen resistance and body composition of orange‐spotted grouper (Epinephelus coioides). Orange‐spotted grouper were fed diets supplemented with levan at concentrations of 0 (control), 5.0, 10.0, 25.0 and 50.0 g kg−1 for 12 weeks. The final weight and per cent weight gain were significantly higher in the 25.0 g kg−1 levan‐supplemented group than in the control group (p < 0.05). All levan‐supplemented diets significantly decreased the count of total viable aerobic bacteria and Vibrio spp. in the intestines of groupers (p < 0.05). Serum total protein levels, globulin levels, lysozyme activity and survival rate of orange‐spotted grouper after challenge with V. harveyi were significantly higher in the 25.0 g kg−1 levan‐supplemented group than in the control group (p < 0.05). This study also found that the 10.0, 25.0 and 50.0 g kg−1 levan‐supplemented diets significantly increased the crude protein level in the body composition of orange‐spotted grouper. Overall, the results of this study indicate that dietary levan (25.0 g kg−1) could be an effective method for enhancing the growth performance and disease resistance in orange‐spotted grouper.

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Partial purification of a Bacillus licheniformis levansucrase producing levan with antitumor activity

Cited by 43 publications

References 30 publications

Immobilization and stabilization of levansucrase biocatalyst of high interest for the production of fructooligosaccharides and levan

Immobilization and stabilization of levansucrase biocatalyst of high interest for the production of fructooligosaccharides and levan

Bacillus spp.: Attractive Sources of Anti-cancer and Anti-proliferative Biomolecules

Effects of dietary levan on growth performance, nonspecific immunity, pathogen resistance and body composition of orange-spotted grouper (Epinephelus coioidesH.)

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