Partial induced reorientation of 5CB in a liquid crystal microarray and a signal-on sensing assay for the detection of aflatoxin B1

Wang, Shihong; Huang, Ting; Zhou, Jun; Chen, Qianshan; Wu, Zhaoyang; Yu, Ru‐Qin

doi:10.1039/d2cc00988a

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…Considering the accelerated HCR kinetics of pTDNs-HCR structures, it was applied in an indirect competitive ELISA for rapid detection of AFB1 (one of the most toxic mycotoxins) (Figure A). − At the detection system, the increased G 4 DNAzyme instead of conventional HRP was bound on the SA that labeled antibody as an amplified signal element, and Nb was used for specifically capturing the target (AFB1). Under the optimized conditions (displayed in Figure S7), the limit of detection (LOD) was calculated to be as low as 0.04 ng/mL (signal-to-noise ratio (S/N) = 3), , which was, respectively, ∼60, 10, and 30 times lower than the I-HCR, TDN-HCR-based ELISA, and traditional ELISA (Figures B,C and S8), it is better than other reported methods for AFB1 detection (Table S2).…”

Section: Resultsmentioning

confidence: 99%

Accelerated Hybridization Chain Reaction Kinetics Using Poly DNA Tetrahedrons and Its Application in Detection of Aflatoxin B1

Li,

Cheng,

Zeng

et al. 2023

ACS Appl. Mater. Interfaces

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Traditional hybridization chain reaction (HCR) as a popular isothermal amplification technique shows some inevitable disadvantages in bioanalysis due to its relatively slow kinetics, which could be markedly promoted when the HCR initiator occurs under tension. Herein, a poly DNA tetrahedrons (pTDNs)-mediated HCR was successfully constructed to make its initiator in a stretched state by long-range electrostatic forces owing to the superimposed electrostatic interactions derived from the synthesized pTDNs, and it was hypothesized that it could remarkably enhance HCR performance, which was testified by theoretical simulations and experimental studies. Consequently, pTDNs-mediated HCR was applied to develop a novel immunoassay for rapid and sensitive detection of aflatoxin B1 as a proof-of-concept, and its signal amplification was attributed to the increased G 4 DNAzyme that loaded on the second antibody. Our work paves a promising way using simple DNA frameworks alone to heighten HCR kinetics for reaction speed improvement and signal amplification in bioanalysis.

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Section: Resultsmentioning

confidence: 99%

Accelerated Hybridization Chain Reaction Kinetics Using Poly DNA Tetrahedrons and Its Application in Detection of Aflatoxin B1

Li,

Cheng,

Zeng

et al. 2023

ACS Appl. Mater. Interfaces

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“…Due to the binding between aptamer and AFB1, complementary ssDNA was released and the degree of orientation of LC increased. This order change can be transformed into micro-spectral optical sensing signals, providing quantitative measurement of AFB1 [ 166 ].…”

Section: Applications In Lc Biochemical Sensorsmentioning

confidence: 99%

State-of-the-Art Development in Liquid Crystal Biochemical Sensors

et al. 2022

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As an emerging stimuli-responsive material, liquid crystal (LC) has attracted great attentions beyond display applications, especially in the area of biochemical sensors. Its high sensitivity and fast response to various biological or chemical analytes make it possible to fabricate a simple, real-time, label-free, and cost-effective LC-based detection platform. Advancements have been achieved in the development of LC-based sensors, both in fundamental research and practical applications. This paper briefly reviews the state-of-the-art research on LC sensors in the biochemical field, from basic properties of LC material to the detection mechanisms of LC sensors that are categorized into LC-solid, LC–aqueous, and LC droplet platforms. In addition, various analytes detected by LCs are presented as a proof of the application value, including metal ions, nucleic acids, proteins, glucose, and some toxic chemical substances. Furthermore, a machine-learning-assisted LC sensing platform is realized to provide a foundation for device intelligence and automatization. It is believed that a portable, convenient, and user-friendly LC-based biochemical sensing device will be achieved in the future.

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Creating liquid crystal microdroplet arrays for multiplexed sensing by spatially-controlled molecular patterning

Chang

Chen

Kar

et al. 2023

Sensors and Actuators B: Chemical

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Partial induced reorientation of 5CB in a liquid crystal microarray and a signal-on sensing assay for the detection of aflatoxin B1

Abstract: A signal-on liquid crystal microarray (LCM) sensor with a micro-spectral optical signal for the detection of AFB1.

Cited by 4 publications

References 25 publications

Accelerated Hybridization Chain Reaction Kinetics Using Poly DNA Tetrahedrons and Its Application in Detection of Aflatoxin B1

Accelerated Hybridization Chain Reaction Kinetics Using Poly DNA Tetrahedrons and Its Application in Detection of Aflatoxin B1

State-of-the-Art Development in Liquid Crystal Biochemical Sensors

Creating liquid crystal microdroplet arrays for multiplexed sensing by spatially-controlled molecular patterning

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