Accelerated Hybridization Chain Reaction Kinetics Using Poly DNA Tetrahedrons and Its Application in Detection of Aflatoxin B1

Abstract

Traditional hybridization chain reaction (HCR) as a popular isothermal amplification technique shows some inevitable disadvantages in bioanalysis due to its relatively slow kinetics, which could be markedly promoted when the HCR initiator occurs under tension. Herein, a poly DNA tetrahedrons (pTDNs)-mediated HCR was successfully constructed to make its initiator in a stretched state by long-range electrostatic forces owing to the superimposed electrostatic interactions derived from the synthesized pTDNs, and it was hypothesized that it could remarkably enhance HCR performance, which was testified by theoretical simulations and experimental studies. Consequently, pTDNs-mediated HCR was applied to develop a novel immunoassay for rapid and sensitive detection of aflatoxin B1 as a proof-of-concept, and its signal amplification was attributed to the increased G 4 DNAzyme that loaded on the second antibody. Our work paves a promising way using simple DNA frameworks alone to heighten HCR kinetics for reaction speed improvement and signal amplification in bioanalysis.

Improved Catalytic Activity of Spherical Nucleic Acid Enzymes by Hybridization Chain Reaction and Its Application for Sensitive Analysis of Aflatoxin B1

“…Additionally, the accuracy of the developed method was also verified by commercial ELISA kits. The test results are shown in Table S4 with acceptable t exp using Student's classic t test (lower than 2.78), demonstrating good accuracy compared to commercial methods [43]. The results presented indicate that the suggested approach demonstrated a satisfactory performance in identifying AFB1 in actual food samples.…”

Multiple Isothermal Amplification Coupled with CRISPR–Cas14a for the Naked-eye and Colorimetric Detection of Aflatoxin B1

“…Researchers are therefore focused on developing new technologies that simplify sample preparation and yield more portable detection devices. These include colorimetric, fluorescent, and electrochemical methods combined with various biosensors for AFB1 detection. − Among these, colorimetry has gained wide acceptance due to its portability, rapid analysis, and high sensitivity. − Magnetic nanoparticles (MNPs) offer advantages such as easy preparation, a large specific surface area, and superparamagnetic properties, making them useful for efficient target enrichment and rapid sample matrix separation . Additionally, gold nanoparticles (AuNPs) exhibit local surface plasmon resonance characteristics; their color change can be used as a biosensor response signal .…”