Electrochemical-Fluorescent Bimodal Biosensor Based on Dual CRISPR-Cas12a Multiple Cascade Amplification for ctDNA Detection
Hehua Zhang,
Hongmin Gao,
Wendi Mu
et al.
Abstract:Circulating tumor DNA (ctDNA) is a critical biomarker
for early
tumor detection. However, accurately quantifying low-abundance ctDNA
in human serum remains a significant challenge. To address this challenge,
we introduce a bimodal biosensor tailored for detecting the epidermal
growth factor receptor (EGFR) mutation L858R in specific nonsmall
cell lung cancer (NSCLC) patients. This biosensor utilizes dual CRISPR-Cas12a
systems to quantify the target via fluorescence and electrochemical
signals. In our system, t… Show more
Set email alert for when this publication receives citations?
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.