“…In contrast to the lobster and the spiny lobster where all subunits are glycosylated, there is only one subunit glycosylated in A. leptodactylus hemocyanin. Subunit b of Astacus hemocyanin, which shows a high degree of sequence similarity to the subunit a of the Panulirus hemocyanin (Gaykema et al, 1984;Bak et al, 1986;Neuteboom et al, 1986Neuteboom et al, , 1992Schneider et al, 1986;Soeter et al, 1986;Jekel et al, 1988;Volbeda and Hoi, 1988) has at the corresponding glycosylation site (Asn-X-Ser/Thr) in domain 1 an exchange of amino acids Asn-Val-Ser from Panulirus to Asn-MetAsp (Schneider ef al., 1986) and is therefore not glycosylated, as demonstrated here. The glycosylation site(s) in A leptodactylus hemocyanin are not yet known, since with the exception of a part of subunit b, no other subunits are sequenced till now.…”