Parenteral Administration of Glutamine Modulates Acute Phase Response in Postparturient Dairy Cows

Abstract

The objective of this study was to investigate whether administration of L-Gln would affect mediators of acute phase response in postparturient dairy cows. Twenty-four multiparous Holstein cows were blocked by the expected day of calving and randomly assigned to 1 of the 3 treatment groups (n = 8/group): 1) i.v. infusion of 10 L of 0.85% NaCl (control), 2) i.v. infusion of 106, or 3) 212 g/d of L-Gln mixed with 10 L of 0.85% NaCl solution; each treatment was given 8 h/d for each of 7 consecutive days starting on d 1 after calving. Blood samples were collected 1 wk before the expected day of parturition as well as on d 0, 7, 14, and 21 after parturition; plasma concentrations of serum amyloid A (SAA), haptoglobin, and lipopolysaccharide-binding protein were measured by ELISA, and alpha(1)-acid glycoprotein was assessed by radial immunodiffusion. Concentrations of SAA, haptoglobin, and alpha(1)-acid glycoprotein increased in control cows after parturition, reaching peak values on d 0 or 7 postpartum (60, 1,093, and 963 microg/mL, respectively). Cows infused with 106 g/d of L-Gln had greater concentrations of SAA in plasma on d 14 and 21 compared with controls (62.8 vs. 30.2 and 71.1 vs. 34.5 microg/mL, respectively). Cows infused with 212 g/d of L-Gln had greater concentrations of SAA on d 7 (82.5 vs. 53.9 microg/mL) and lower concentrations of haptoglobin on d 14 and 21 postpartum compared with controls (264 vs. 621 and 175 vs. 587 microg/mL, respectively). Cows treated with 106 and 212 g/d of L-Gln had greater plasma lipopolysaccharide-binding protein concentrations on d 7 compared with control group (50.0 and 35.6 vs. 10.8 microg/mL, respectively). There were no treatment differences with respect to milk yield and DM intake during the experimental period. In conclusion, our data indicate that i.v. administration of L-Gln modulated acute phase mediators in dairy cows after parturition and warrants further research into the mechanisms behind these effects.

Effects of dietary supplementation of glutamine and mannan oligosaccharides on plasma endotoxin and acute phase protein concentrations and nutrient digestibility in finishing steers

“…Therefore, further studies are warranted to verify the appropriate dose of Gln for steers. This study showed that Gln supplementation significantly increased LBP, which was in accordance with the study of Jafari et al (2006) where dairy cows were infused with 106 g/d and 212 g/d Gln. LBP can promote LPS to transfer to CD-14 to activate mononuclear macrophages and endothelial cells, which in turn activates Kupffer cells in liver to clear LPS via Toll-like receptors-4 (Purohit et al 2008).…”

“…LBP can promote LPS to transfer to CD-14 to activate mononuclear macrophages and endothelial cells, which in turn activates Kupffer cells in liver to clear LPS via Toll-like receptors-4 (Purohit et al 2008). Furthermore, LBP can transfer LPS to serum lipoproteins, such as low-density lipoprotein (LDL) and highdensity lipoprotein (HDL), for clearing LPS from the bloodstream (Jafari et al 2006). Ceciliani et al (2012) indicated that high concentrations of LBP play an anti-inflammatory role, thus LBP can be regarded as a defense strategy against LPS in disproportionate inflammatory response.…”

Comparative proteomics analysis of plasma proteins during the transition period in dairy cows with or without subclinical mastitis after calving

“…In addition, serum amyloid A is a family of proteins encoded in a multigene complex and is one of the acute-phase reactants in response to infection, inflammation, and trauma (Jensen and Whitehead, 1998). Serum amyloid A in periparturient dairy cows was measured by ELISA and it was found to increase dramatically after calving (Meglia et al, 2005;Jafari et al, 2006). In our study, isoforms of serum amyloid A were found to be altered in plasma before calving in cows with or without new intramammary infection.…”

Utilização da glutamina no tratamento de lesões de isquemia e reperfusão no jejuno em eqüinos

“…No entanto, a dose utilizada não atenuou as lesões decorrentes de I/R, devendo-se considerar doses maiores para tal finalidade. Na avaliação da diminuição da resposta inflamatória pós-parto em bovinos leiteiros, a glutamina foi utilizada nas doses de 159 e 319mg/ kg, tendo ambas igual eficácia (Jafari et al 2006).…”