Parentage and relatedness determination in farmed Atlantic salmon (Salmo salar) using microsatellite markers

Norris, Ashie; Bradley, Daniel G.; Cunningham, E. P.

doi:10.1016/s0044-8486(99)00247-1

Cited by 172 publications

(95 citation statements)

References 16 publications

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“…It has a high degree of individual diversity and co-dominance, and is used for evaluation of genetic diversity and phylogenetic analysis in aquatic organisms. (Norris, Bradley & Cunningham 2000;Li, Park, Endo & Kijima 2004;Barroso, Hilsdorf, Moreira, Cabello & Traub-Cseko 2005).…”

Section: Introductionmentioning

confidence: 99%

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Han¹,

Lee²,

Jung³

et al. 2017

GenAqua

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Turbot (Scophthalmus maximus, Turbot) is a European flounder species, characterized by the absence of scales on the epidermis and the presence of small protuberances. Its capacity to be cultured effectively in fish farms at high densities makes it appealing in the aquaculture industry specifically in the UK, France and Spain. In recent years, development of a new strain by managing genetic resources is being intensively studied in Korea. It is a necessity in aquaculture to check the genetic diversity of a certain species in order to propagate better performing generations of its species. Microsatellite refers to 2-4 nucleotide repeats throughout genome with highly diverse repeat numbers, allowing of which to be used as biological marker for population identification and evaluation of genetic diversity. Population genetic analysis was carried out through the basis of 36 alleles which are different among the groups, presented by eleven markers that could be used in differentiating the genetic platforms of farmed turbot populations from Korea, France, and Turkey. The results obtained can be used in developing markers that are beneficial in identifying superior traits which in turn can be helpful in securing genetic diversity. In conclusion, this study identified genetic differences between farmed turbots in France and Turkey against the S. Korea. Based on the results, it can be implied that the genetic pool is capable of being expanded, and thereby maintenance of genetic diversity and improvement of excellent traits would be possible.

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Section: Introductionmentioning

confidence: 99%

Untitled

Han¹,

Lee²,

Jung³

et al. 2017

GenAqua

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“…In addition, reproduction procedures should be designed to minimize the loss of genetic variation. Maximal retention of genetic variability can be achieved through monitoring the pedigree structure of descendants and suitably selecting reproducers among unrelated descendants (Norris et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial and microsatellite DNA analyses showed comparative genetic diversity between parent and offspring populations of Korean black rockfish in a hatchery facility

An¹,

Lee²,

Park³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The black rockfish, Sebastes inermis (Sebastidae), is an important commercial fishery resource in Korea. As a preliminary investigation into the effect of artificial reproduction in a hatchery facility, the genetic divergence between parent and offspring populations of black rockfish was accessed using 10 polymorphic nuclear microsatellite DNA loci and a mitochondrial (mt) control gene. All loci that were screened showed marked polymorphisms. mtDNA control region sequences were also highly variable. Of approximately 350 base pairs (bp) sequenced, 52 variable sites, comprising 56 base substitutions, were found among 233 individuals. Offspring populations showed less genetic variability than the parent population in terms of numbers of microsatellite alleles and mtDNA haplotypes, as well as mtDNA haplotype diversity. Statistical analysis of the fixation index (Φ ST and F ST ) and analysis of molecular variance using both DNA markers showed significant genetic differences between the parent and offspring populations. These results suggest that random genetic drift and/or inbreeding events, as well as artificial selection and founder effects, occurred when the offspring strain was reproduced in a hatchery facility despite thousands of males and females from different hatcheries being maintained for artificial reproduction. Therefore, it is necessary to improve current hatchery programs by monitoring genetic variation in both the broodstock and progeny and controlling inbreeding within stocks in commercial breeding facilities to maintain the production of high-quality black rockfish. This information will be useful for determining suitable guidelines for establishing and maintaining cultured stocks and the aquaculture industry of S. inermis.

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“…More recently, the sequencing of the ends of Bacterial Artificial Chromosome (BAC) clones has provided a rich source of microsatellites Phillips et al, 2009). Microsatellites have been used extensively to examine the population structure of Atlantic salmon (e.g., King et al, 2001) as well as for assigning parentage and relatedness of individuals in a mixed family stock (Norris et al, 2000), which precludes the necessity of rearing families in individual tanks or using physical tags. Many of the microsatellite markers that have been developed for one species of salmonid can be used in others (e.g., McGowan et al, 2004;Presa & Guyomard, 1996;Scribner et al, 1996), which enables genomic comparisons among salmonids such as Atlantic salmon, brown trout (Salmo trutta), rainbow trout and Arctic charr Woram et al, 2004).…”

Section: Microsatellite Markersmentioning

confidence: 99%