In mitochondrial (mt) DNA haplotyping for populations of Japanese ‰ounder, the nucleotide sequences of thê rst-half control region (FH CR) have been frequently used because of the high sequence variability. We studied the possibility of improving the accuracy of mtDNA haplotyping by adding sequence data of ND2 and ND5 genes.Theˆnest resolution of haplotypes was obtained when concatenated sequences of FH CR and ND2 were used. Thus, mtDNA haplotyping based on the simultaneous use of the two mtDNA regions will provide an e‹cient means to precisely evaluate the genetic diversity of Japanese ‰ounder in relation to the stock enhancement program.
キーワードND2 遺伝子,調節領域,ハプロタイプ,ヒラメ,ミトコンドリア