Parathyroid Hormone (1-34) Augments Spinal Fusion, Fusion Mass Volume, and Fusion Mass Quality in a Rabbit Spinal Fusion Model

O’Loughlin, Padhraig F.; Cunningham, Matthew E.; Bukata, Susan V.; Tomin, Emre; Poynton, Ashley R.; Doty, Stephen B.; Sama, Andrew A.; Lane, Joseph M.

doi:10.1097/brs.0b013e318191e687

Cited by 105 publications

(91 citation statements)

References 66 publications

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“…Indeed, Nozaka et al (2008) reported a 5.3-fold reduction in osteoclast number. However, other studies by O'Loughlin et al (2009) and reported contrasting results with a 2.5-to 4-fold increase in osteoclast number. Markers of bone formation such as osteocalcin and alkaline phosphatase were also shown to be increased between 1.2 and 3.1 fold (Komrakova et al, 2011;Qiu et al, 2013), indicating upregulation of osteoblast activity.…”

Section: Direct Administration Of Pthmentioning

confidence: 44%

“…As previously mentioned, some studies have investigated cartilage formation as a precursor to bone tissue generation. Following PTH treatment chondrogenesis/cartilage formation was increased 3 to 9.9 fold (Kakar et al, 2007;O'Loughlin et al, 2009) leading to enhanced trabeculated callus formation . Bone architecture and structure are important quality indicators, yet many studies report only simple measurements of bone quantity.…”

Section: Direct Administration Of Pthmentioning

confidence: 99%

See 1 more Smart Citation

Tissue engineered bone using select growth factors: A comprehensive review of animal studies and clinical translation studies in man

Gothard¹,

Smith²,

Kanczler³

et al. 2014

eCM

154

121

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Section: Direct Administration Of Pthmentioning

confidence: 44%

Section: Direct Administration Of Pthmentioning

confidence: 99%

Tissue engineered bone using select growth factors: A comprehensive review of animal studies and clinical translation studies in man

Gothard¹,

Smith²,

Kanczler³

et al. 2014

eCM

154

121

View full text Add to dashboard Cite

“…The aim of this study was to achieve a fusion rate of less than 40 % and then determine whether autologous bone marrow augmented arthrodesis. Other studies subsequently used this bone paucity model to provide a larger potential for improved fusion rates with a variety of bone graft substitutes [13][14][15][16][17][18][19][20][21][22]. While our study found a significant positive relationship between volume of autograft and fusion rates, a tenfold range of volume of autograft (1.4-12 cm 3 ) was used in the studies evaluated.…”

Section: Discussionmentioning

confidence: 79%

Factors influencing arthrodesis rates in a rabbit posterolateral spine model with iliac crest autograft

et al. 2013

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Purpose The rabbit posterolateral intertransverse spine arthrodesis model has been widely used to evaluate spinal biologics. However, to date, the validity and reproducibility of performance of iliac crest bone graft, the most common and critical control group, has not been firmly established. We evaluated original research publications that utilized this model, identified which experimental conditions affected fusion rates, and developed an algorithm to predict fusion rates for future study designs. Methods A MEDLINE search was performed for publications through December, 2011 that utilized this model to evaluate fusion rates elicited by iliac crest autograft. All study parameters were recorded, and logistic regression analyses were performed to determine the effects of these variables on fusion rates as determined by either manual palpation or radiographs. Results Seventy studies with 959 rabbits in 102 groups met the inclusion criteria. Excluding studies that measured fusion at 4 or fewer weeks or intentionally tried to decrease the fusion rate, the overall fusion rate for autograft was 58.3 ± 16.3 % (mean ± SD) as determined by manual palpation and 66.4 ± 17.8 % by plain radiographs. Regression analysis demonstrated a difference between these outcome measures with a trend towards significance (p = 0.09). Longer time points and larger volumes of autograft resulted in significantly greater reported fusion rates (p \ 0.0001 and p \ 0.05, respectively). Neither strain, age, weight, nor vertebral level significantly affected fusion rates. Conclusions Although experimental conditions varied across studies, time point evaluation and autograft volume significantly affected fusion rates. Despite some variability demonstrated across certain studies, we demonstrated that when the time point and volume of autograft were controlled for, the iliac crest control group of the rabbit posterolateral spinal arthrodesis model is both reliable and predictably affected by different experimental conditions.

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“…The sample size estimation was based on data from a previous study by O'Loughlin et al [31] who reported a fusion rate of 80% in the treated group and 30% in the control group. To obtain a similar difference, with a significance level of 5% and a power of 80%, the smallest appropriate sample size was 15 animals in each group.…”

Section: Methodsmentioning

confidence: 99%

“…Autologous cortico-cancellous iliac crest bone graft was obtained through the same skin incision, but through a different fascial approach. In order to reduce the fusion rate described for this model (since a higher fusion rate makes it difficult to power a study to demonstrate a statistical difference), we chose to harvest a smaller graft volume, which was demonstrated to decrease fusion rates [12,31]; the graft volume used was 1.5 cc per side. Bone graft was applied between the decorticated intertransverse processes.…”

Section: Methodsmentioning

confidence: 99%

Effect of a single dose of pamidronate administered at the time of surgery in a rabbit posterolateral spinal fusion model

et al. 2010

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Spinal fusion is usually performed on patients who receive bisphosphonates (BP); however, limited data on their action on spinal fusion are available. Previous studies in animal models have shown that chronic administrations of BP reduced spinal fusion rates, and only one study has shown that a single dose administration of zolendronic acid increased fusion rate. The objective of the present study was to evaluate if pamidronate (PA), which was previously demonstrated to reduce spinal fusion rate when administered continuously for 8 weeks, would increase the spinal fusion rate if administered in a single dose at the time of surgery in a rabbit model. Thirty-two New Zealand rabbits underwent an L5-L6 posterolateral intertransverse fusion with iliac crest autograft. Animals were randomized to receive either PA 3 mg/kg in a single dose immediately after surgery, or normal saline. Animals were killed 8 weeks after surgery and fusion was determined by manual palpation and radiographic analysis. Fusion healing was obtained in eight rabbits (50%) in the PA group and in four animals (25%) in the control group, p = 0.137. In a rabbit model, a single dose of PA did not decrease lumbar spinal arthrodesis consolidation rates, but it obtained a nonsignificant higher spinal fusion rate.

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Parathyroid Hormone (1-34) Augments Spinal Fusion, Fusion Mass Volume, and Fusion Mass Quality in a Rabbit Spinal Fusion Model

Abstract: Intermittent PTH administration increased posterolateral fusion success in rabbits. Fusion bone mass and histologic determinants were also improved with PTH treatment. PTH has promise for use as an adjunctive agent to improve spinal fusion in clinical medicine.

Cited by 105 publications

References 66 publications

Tissue engineered bone using select growth factors: A comprehensive review of animal studies and clinical translation studies in man

Tissue engineered bone using select growth factors: A comprehensive review of animal studies and clinical translation studies in man

Factors influencing arthrodesis rates in a rabbit posterolateral spine model with iliac crest autograft

Effect of a single dose of pamidronate administered at the time of surgery in a rabbit posterolateral spinal fusion model

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