Paragangliomas: Neuroendocrine features and cytometric DNA distribution patterns

Abstract

Paragangliomas from 22 patients with extraadrenal tumours of this type were studied. Neuroendocrine features were examined using immunohistochemical techniques. Twenty-two antisera raised against neuroendocrine "markers", regulatory peptides, serotonin and intermediate filament proteins were studied in this group and cytometric DNA assessments were made by means of image cytometry. One normal and 5 hyperplastic carotid bodies were used as controls in the DNA cytometric investigations. Clinical and/or histopathological evidence of "malignancy" was present in 5 cases. The tumour cells showed heterogeneity with regard to their expression of different peptides, and the immunohistochemical analyses did not permit differentiation between benign and malignant paragangliomas. An euploid nuclear DNA distribution pattern was found in all controls and in 17 of the tumours; all except 1 were clinico-pathologically benign. An aneuploid DNA pattern was observed in 5 of the cases and some malignant features were present in 4 of these cases. DNA data may give further information apart from that obtained from the histopathological findings which may be of value in predicting the biological behaviour of this tumour type.

A Functioning-Desensitization Paraganglioma Which Caused Hemodynamic Instability During Tumoral Resection: Report of a Case

“…The prognosis deteriorated progressively with a decrease in the number of sustentacular cells. 12 Sustentacular cells, which are located either at the periphery of nests of chief cells or are dispersed between the tumor cells, were graded semiquantitatively by cell density unit area counting the S-100 protein-positive cells. In our patient there was slightly more reaction to neuron-specific enolase and a weak S-100 protein antibody reactivity; therefore, we surmised that the prognosis of this patient may not be so favorable, and we thus plan to follow him more frequently than similar patients.…”

Bilaterales Paragangliom des Nervus vagus

“…Letzte Studien haben belegt, dass sich bei der Mehrheit der untersuchten Paragangliome (fast 100%) die typischen neuroendokrinen Marker nachweisen lassen [11]. Letzte Studien haben belegt, dass sich bei der Mehrheit der untersuchten Paragangliome (fast 100%) die typischen neuroendokrinen Marker nachweisen lassen [11].…”

“…Weiterhin kann in Paragangliomen das Protein PG 9.5 nachgewiesen werden, welches als ausgezeichneter neuroendokriner Marker breiten Spektrums gilt [11], sowie Neurofilament, Serotonin, Somatostatin und verschiedene andere Peptidhormone [14].…”

“…Dies würde u. U. eine Tracheotomie oder gar Laryngektomie aus funktionellen Gründen und/oder eine perkutane endoskopische Gastrostomie zur Ernährung erfordern [9,11,12]. Dies würde u. U. eine Tracheotomie oder gar Laryngektomie aus funktionellen Gründen und/oder eine perkutane endoskopische Gastrostomie zur Ernährung erfordern [9,11,12].…”