Paracrine Interactions in the Anterior Pituitary

Robberecht, Wim; Denef, Carl

doi:10.1007/978-1-4612-3478-4_3

Neuroendocrine Perspectives

1989

DOI: 10.1007/978-1-4612-3478-4_3

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Paracrine Interactions in the Anterior Pituitary

Wim Robberecht¹,

Carl Denef²

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Cited by 3 publications

References 54 publications

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Morphometrical variations of prolactin cells in response to prolonged and systemic administration of Met-enkephalin in female rats

et al. 1992

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A stimulatory effect on prolactin secretion had been describe after acute and systemic administration of met-enkephalin, but the effects of this opioid after chronic administration has not been reported, and the response of mammotroph cells is not clear. As a complement to previous studies, a morphometric analysis (light and electron microscopy) was carried out on prolactin cells from female rats treated chronically with met-enkephalin. Clear features of cellular hyperactivity appeared after chronic and systemic administration of the opioid, and these persisted for two weeks. The changes consisted in increases of cellular, cytoplasmic and nuclear areas, volume and surface densities of the Golgi complex and rough endoplasmic reticulum, as well as the numbers of exocytotic figures. These morphological alterations were paralleled by an increase in serum prolactin levels as detected by RIA. It is concluded that the increase in the synthesis and secretory activity of prolactin cells following chronic and systemic administration of met-enkephalin is very similar to those observed after acute and intraventricular administration.

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Morphometrical variations of prolactin cells in response to prolonged and systemic administration of Met-enkephalin in female rats

et al. 1992

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In vitro immunoblockade of VIP inhibits the proliferation of pituitary prolactin cells

et al. 2005

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VIP is a peptide synthesised in the pituitary gland and is involved in the stimulation of prolactin secretion. However, to date it has not been determined whether VIP is able to regulate the proliferation of pituitary prolactin-producing cells, like other factors involved in the regulation of prolactin such as estradiol or dopamine. The aim of the present study was to address whether VIP is involved in regulating the proliferation of pituitary prolactin-secreting cells. Thus, we performed an in vitro study on monolayer cultures of rat pituitary cells, neutralising the possible paracrine effect of VIP by immunoblockade of the peptide and later determining the degree of proliferation of prolactin-secreting cells. The effects of immunoblockade were validated by determining the levels of VIP in the culture media, which were decreased (P < 0.01), and modifications in the patterns of the immunohistochemical reaction to prolactin-positive cells. Immunoblockade of VIP decreased the proliferation of pituitary prolactin-positive cells at all antibody concentrations analysed, mainly between 3 and 12 h (P < 0.01). Moreover, immunoblockade decreased the sizes of the cellular and nuclear areas, except at 1 h, at which point it only decreased the nuclear area of prolactin-positive cells. The results obtained suggest that-in the same way as it regulates the secretion of the hormone-VIP could be involved in regulating the proliferation of prolactin cells, like estradiol or dopamine.

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In vivo and in vitro evidence of growth hormone-releasing factor-like produced locally in the adenohypophyseal cells of the rat

et al. 1991

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Paracrine Interactions in the Anterior Pituitary

Cited by 3 publications

References 54 publications

Morphometrical variations of prolactin cells in response to prolonged and systemic administration of Met-enkephalin in female rats

Morphometrical variations of prolactin cells in response to prolonged and systemic administration of Met-enkephalin in female rats

In vitro immunoblockade of VIP inhibits the proliferation of pituitary prolactin cells

In vivo and in vitro evidence of growth hormone-releasing factor-like produced locally in the adenohypophyseal cells of the rat

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