In vivo and in vitro evidence of growth hormone-releasing factor-like produced locally in the adenohypophyseal cells of the rat

Carretero, José; Sánchez, Francisco Javier Sierro; Vázquez, Ricardo; Cacicedo, Lucinda; Sánchez‐Franco, Franco; Fernandez, G.; Montero, Manuel

doi:10.1016/0143-4179(91)90088-z

Cited by 4 publications

(1 citation statement)

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“…Mechani cal dispersion was achieved by passing pituitary' glands through Pas teur pipettes and 20-to 22-gauge needles. After centrifugation, the supernatant was removed and the cells were resuspended in an appropriate volume of Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% calf serum, 2.5% fetal calf serum, 2% /,-glutamine, 1,000 IU/ml of penicillin and 1.000 IU/ml of streptomycin [8][9][10]. Cells were seeded on culture dishes(30 x 15m m )at a final concentration of 5 x 105 cells/1.5 ml/dish and incubated at 37°C in a 5% CO:/95% air atmosphere for 7 days.…”

Section: Methodsmentioning

confidence: 99%

Decreases in the Size and Proliferation Rate of VIP-lmmunoreactive Cells Induced in vitro by Testosterone Are Associated with Decreases in VIP Release

Carretero

Rubio²,

Vázquez-Perfecto³

et al. 1997

Neuroendocrinology

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An in vitro study was carried out to determine whether testosterone directly inhibits the secretion of pituitary vasoactive intestinal peptide (VIP). A study was also conducted to elucidate the repercussions of treatment with 10-⁶M testosterone of monolayer cultures in the size and proliferation rate of pituitary VIP-immunoreactive cells, using double immunocytochemical labelling for proliferating cell nuclear antigen (PCNA) and VIP. Testosterone decreased the in vitro release of VIP from pituitary cells. It also decreased the percentage of pituitary VIP-immunoreactive and PCNA- and VIP-immunoreactive cells and decreased the size of the cellular and nuclear areas of these cells. Our results suggest that the inhibition of pituitary VIP secretion induced by testosterone could be exerted at pituitary level and that testosterone could act as a modulator of the proliferation rate of rat pituitary VIP-immunoreactive cells.

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Section: Methodsmentioning

confidence: 99%