Papierelektrophoretische trennung der mucoide des perchlorsäurefiltrates

Kaltwasser, F; Wolters, P; Pieper, Josef

doi:10.1016/0009-8981(66)90063-5

Cited by 3 publications

(1 citation statement)

References 27 publications

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“…But there is disadvantage in the use of precipitating agents as artefacts may appear. Our method is similar to the method of Kaltwasser et al [7]. These authors were able to fractionate the native serum mucoproteids of PC1A filtrates using paper electrophoresis.…”

Section: Discussionmentioning

confidence: 72%

The Urinary Trypsin Inhibitors in Some Diseases

Nowak¹

1968

Enzymol Biol Clin

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Summary. Urinary trypsin inhibitors (TI) were differentiated by the electrophoretic fractionation as well as by selective inactivation by heating, acidification, acetone and chloroform respectively. The labile TI has been found in urine of patients with nephrotic syndrome. It moves with α(1)-globulin fraction and its excretion corresponds to the degree of proteinuria. The urinary excretion of the stable, PCIA-soluble TI in renal diseases does not depend on the degree of proteinuria. The highest levels of this inhibitor may be observed in renal insufficiency. It moves with albumins. The same electrophoretic mobility has the inhibitor found in urine in various other diseases (acute inflammations, cancer, obstructive jaundice) and in healthy persons. In some cases two electrophoretic fractions of the stable TI have been found : the fast and the slow one, moving with albumins and β-γ-globulins resp., but there is not ultimate proof, that they are separate entities. The protein precipitation by PC1A is a simple, good method for isolation of the stable, PCIA-soluble inhibitor from others filtrated to urine in patients with proteinuria.

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Section: Discussionmentioning

confidence: 72%