Pantropic retroviral vectors integrate and express in cells of the malaria mosquito, Anopheles gambiae.

Matsubara, Tomoyo; Beeman, Richard W.; Shike, Hiroko; Besansky, Nora J.; Mukabayire, O.; Higgs, Stephen; James, Anthony A.; Burns, Jane C.

doi:10.1073/pnas.93.12.6181

Cited by 66 publications

(43 citation statements)

References 22 publications

(11 reference statements)

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“…For example, the murine Fv-1 gene appears to inhibit preintegration complex entry into the nucleus by mimicking gag proteins (57)(58)(59). However, the transduction vectors used herein are stripped of most viral sequences and all structural genes, suggesting either that the pol gene products (reverse transcriptase and integrase proteins) that are packaged with the viral genome somehow mediate resistance to superinfection, or that there is a cellular contribution to the control of PRV copy number in transduced sea urchin eggs (our data) and other cell types (29,31,33,55,56).…”

Section: Discussionmentioning

confidence: 97%

“…Thus, viruses pseudotyped with vsvG bypass receptor-mediated cell entry, and instead are able to infect any cell type via fusion of the viral envelope with the plasma membrane (26). Indeed, PRVs infect a wide range of unusual model organisms, including clams, shrimp, oysters, arthropods, amoebae, and zebrafish (27)(28)(29)(30)(31)(32)(33)(34). Here we show that PRVs infect sea urchin embryos, integrating genomically with a copy number of one per genome.…”

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confidence: 97%

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Pantropic retroviruses as a transduction tool for sea urchin embryos

Core¹,

Reyna²,

Conaway³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Sea urchins are an important model for experiments at the intersection of development and systems biology, and technical innovations that enhance the utility of this model are of great value. This study explores pantropic retroviruses as a transduction tool for sea urchin embryos, and demonstrates that pantropic retroviruses infect sea urchin embryos with high efficiency and genomically integrate at a copy number of one per cell. We successfully used a self-inactivation strategy to both insert a sea urchin-specific enhancer and disrupt the endogenous viral enhancer. The resulting self-inactivating viruses drive global and persistent gene expression, consistent with genomic integration during the first cell cycle. Together, these data provide substantial proof of principle for transduction technology in sea urchin embryos.

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Section: Discussionmentioning

confidence: 97%

mentioning

confidence: 97%

Pantropic retroviruses as a transduction tool for sea urchin embryos

Core¹,

Reyna²,

Conaway³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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“…Alternatif metode transfer gen untuk spesies seperti itu telah dikembangakan oleh Burns et al (1993) dengan menggunakan bantuan sebuah vektor yang dikenal sebagai replication-defective pantropic retroviral. Vektor ini telah menunjukkan hasil yang efektif dalam menginfeksi sel lines ikan, kadal air, kodok (Xenopus) dan nyamuk (Burns et al 1993Matsubara et al 1996), dan telur ikan yang baru dibuahi seperti medaka, zebra dan kerang, Mulina lateralis (Burns et al 1993;Lin et al 1994;Lu at al. 1996Lu at al.…”

Section: Metode Alternatifunclassified

Aplication of Gene Transfer in Aquaculture

Ali

Yoshizaki

Carman

et al. 2007

Jurnal Akuakultur Indonesia

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Recently, global food security has become a hot issue by the public in national as well as out of the country. Aquacultural output will need to be increased several fold in order to meet the rising demands for fish in coming years as the increasing of mankind population. The intensity and capacity of production is expected to increase using biotechnology approach. One of the advances biotechnologies that expected to be a powerful approach for aquaculture development is transgenic technique. This technique has been applied to several commercially valuable species. This review describes various techniques of gene transfer, persistence and expression of transferred gene, application and future aspect of gene transfer research in aquaculture.Key words: Gene transfer, gene expression, biotechnology, aquaculture ABSTRAKSaat ini, keamanan pangan telah menjadi isu hangat di masyarakat baik di dalam maupun di luar negeri. Produksi akuakultur diharapkan dapat ditingkatkan beberapa kali lipat untuk memenuhi kebutuhan pangan berupa ikan dimasa-masa mendatang akibat peningkatan populasi manusia. Intensitas dan kapasitas produksi diharapkan meningkat dengan menggunakan pendekatan bioteknologi. Salah satu teknik modern yang diduga akan menjadi sarana yang berguna dalam pengembangan akuakultur adalah teknologi transfer gen. Teknik ini telah diaplikasikan pada spesies-spesies yang memiliki nilai ekonomis. Ulasan ini menggambarkan variasi metode transfer gen, persistensi dan ekspressi dari gen yang ditransfer, aplikasi dan prospeknya ke depan dari penelitian transfer gen dalam akuakultur.Kata kunci : transfer gen, ekspressi gen, bioteknologi, akuakultur PENDAHULUANKeamanan pangan telah menjadi isu utama saat ini baik di dalam maupun di luar negeri. Dengan perkiraan jumlah populasi dunia mencapai 11 milyar pada tahun 2050, sektor pertanian (termasuk perikanan) ditantang untuk bisa melipatgandakan produksi pangan di tahun 2025 dan tiga kali lipat di tahun 2050, dengan kondisi lahan dan air per kapita berkurang dan tantangan kondisi lingkungan meningkat. Peningkatan produksi perikanan, dalam hal ini seafood, diperlukan sekitar 7 kali lipat di tahun 2020 (Hew & Fletcher 1997). Produksi pertanian yang berbasis lahan, dalam arti luasan dan output per hektar, telah mencapai titik maksimum dengan melakukan pemupukan dan selektif breeding. Hal tersebut diperburuk oleh bertambah jeleknya kualitas tanah, air dan udara, yang disebabkan oleh perubahan iklim global, penggundulan lahan, polusi,dan industrialisasi .Perkembangan ilmu biologi molekuler akhir-akhir ini telah memungkinkan dengan mudah membuat klon suatu gen yang diinginkan. Pada tahun 1980, Gordon et al. (1980) melaporkan keberhasilan memindahkan gen ke tikus dengan cara menyuntikkannya ke dalam pronukleus telur yang sudah dibuahi. Tikus tersebut selanjutnya dinamakan "tikus transgenik". Dengan menggunakan teknik ini, suatu gen yang menkodekan karakter tertentu yang diinginkan dapat diintroduksi ke suatu individu. Sekali gen asing terintegrasi ke dalam genom resipien, gen te...

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“…For transduction, cells were incubated with serial dilutions of virus supernatants in the presence of 8 g/ml polybrene (Sigma) and kept for a further 3 days. For X-gal staining transduced 3Y1 was fixed with 1.25% glutaraldehyde and stained with 5 mm K 4 [Fe(CN) 6 ], 5 mm K 3 [Fe(CN) 6 ], 2 mm MgCl 2 and 1 mg/ml X-gal (5-bromo-4-chloro-3-indolyl-␤-d-galactopyranoside) (Wako, Osaka, Japan) for more than 4 h. The numbers of cellular clones with blue-stained nuclei were counted. Expression of p53 (human) protein was evaluated by immunocytochemical staining using monoclonal anti-p53 (human) IgG (DO-1; Santa Cruz) and visualized as described above.…”

Section: Retrovirus Transduction and Titrationmentioning

confidence: 99%

“…Recently, retrovirus vectors pseudotyped with the G protein of vesicular stomatitis virus (VSV-G) have been constructed and shown to be advantageous in that they have a much broader host range, often yielding higher transduction efficiency, than conventional amphotropic retrovirus vectors and that virus stocks can be concentrated by ultracentrifugation to give a very high titer (approximately 1 × 10 9 IU/ml). [4][5][6][7] Efficient production systems for these vectors have recently been developed by us 8 and by other groups, [9][10][11] making use of the CreloxP-mediated recombination system and inducible promoters, respectively.…”

Section: Introductionmentioning

confidence: 99%