ABS'IBAC,!:EXPERn~TAL PULMONARY EMPHYSEMA IN DOGS by RUNGSUN PUSHPAKOM, M.D.Varying degrees of panlobular emphysem were produced in 10 dogs by intratracheal injection of papain. Pulmonary function was studied before and after the development of emphyserna. The production of emphysema led to an increase in ERC, RV and TIC of 23%, 24%, and 16% respectively.'fhe arterial oxygen saturation decreased by 8% and the Pao2 by 19 mrnHg.There was no significant effect on the Paco2' pH and bicarbonate. Steady state diffusing capa city was reduced by 55%, compliance increased by 63% Mr. L. Bartett's technical advice in designing and setting up the equipment for measuring the diffusing capacity was invaluable.Other technical help was provided by Mrs. L. Hoolwere, and Mrs. J.Pedneault who performed the blood gas analyses and Miss L. Lafontaine for preparing histological sections and Miss M. Charbonneau for preparing paper mounted whole lung sections. Miss G. E. Angus was responsible for the assessment of the internal surface area of the lung. The illustrations were done by Mr. M. Wanngeon.Very special thanks are extended to Mr. S. Vongpaisal and Mr.S. Nepszy for their assistance in so many ways to complete this thesis.Finally, thanks are due to Miss J. Lukjaniec for the many pains taken during the task of typing this thesis.(ii) ABSTRACT Varying degrees of pan1obu1ar emphysema was produced in 10 dogs by intratrachea1 injection of papain. Pu1monary function was studied before and after the deve10pment of emphysema. The production of emphysema 1ed to an increase FRC, RV and TLC of 23%, 24%, 16% respective1y. The arteria1 oxygen saturation decreased by 8% and the Pao 2 by 19 mmHg. There was no significant effect on the Paco 2 , pH and bicarbonate. Steady state diffusing capacity was reduced by 55, comp1iance increased by 63% and there was a shift in the static pressure volume curve of the 1ung upward and to the 1eft. The re1ationship between pu1monary resistance, ~, and 1ung volume was not significant1y changed. However, fo11owing excision of the 1ungs, ~ was partitioned by the retrograde catheter technique and the periphera1 resistance was abnorma11y high accounting from 33 to 84 of~. There was a decrease in the resistance of the central airways exp1aining the preservation of a norma1~. Trivial to moderate pan1obu1ar emphysema was found in the anima1s and was usua11y fair1y loca1ized in 2xtent. Mean 1inear intercept ranged from 0.124 to 0.258 (normal 0.009 -0.149). The a1veo1ar surface area at a standard 1ung volume was reduced to 33.0 -57.0 (normal 59 -71). With exception of the FRC there was no significant corre1a-tian between any of the function tests and the degree of emphysema. The data presented in this thesis represents an attempt ta crea te such a model in the dog.-2-
Histological ReviewSince vesicu1ar and interstitial emphysema was first recognized and described by Laennec (66), in 1819, a number of studies on the disease have been reported. Many attempts to produce emphysema experimenta11y have been carried out on a...