Pandora box of BCA assay. Investigation of the accuracy and linearity of the microplate bicinchoninic protein assay: Analytical challenges and method modifications to minimize systematic errors

Rogatsky, Eduard

doi:10.1016/j.ab.2021.114321

Cited by 26 publications

(15 citation statements)

References 10 publications

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“…For PhMV, there was no discernable profile of elution. Because the BCA signal should be insensitive to the protein sequence, these differences are more likely due to more adsorption and retention of these nanoparticles in MTS . These results are consistent with prior work, showing that TMGMV has exceptional mobility when compared to synthetic nanoparticles and other plant viruses …”

Section: Resultssupporting

confidence: 89%

“…Because the BCA signal should be insensitive to the protein sequence, these differences are more likely due to more adsorption and retention of these nanoparticles in MTS. 31 These results are consistent with prior work, showing that TMGMV has exceptional mobility when compared to synthetic nanoparticles and other plant viruses. 18 Parsing out some properties that may affect the soil mobility of these plant viruses, we consider their shape, surface chemistry, and structural rigidity.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Plant Viral Nanocarrier Soil Mobility as a Function of Soil Type and Nanoparticle Properties

Venkateswaran¹,

Caparco²,

González-Gamboa³

et al. 2023

ACS Agric. Sci. Technol.

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The use of nanoparticles for agrochemical delivery is an important step toward achieving global food security. Specifically, the ability to target the delivery of pesticides and other useful chemicals into the soil will greatly improve the efficiency and efficacy of these molecules, mitigating crop losses associated with pests and parasitic organisms. While synthetic nanoparticles can be a good delivery vehicle and demonstrate high mobility in the soil, their fate and persistence have some implications for human and environmental health. Therefore, using proteinaceous materials such as plant virus nanoparticles, which have already been adapted for the soil, provides a fruitful avenue of exploration. Previously, tobacco mild green mosaic virus (TMGMV) and red clover necrotic mosaic virus (RCNMV) have shown high soil mobility and nematicide delivery for the treatment of plant parasitic nematodes. To further the use of these plant virus nanoparticles in soil delivery applications, understanding the properties of the soil and the nanoparticles is essential. In this work, we assessed the mobility of TMGMV, potato virus X, and tobacco mosaic virus with a genetically encoded lysine on its surface (TMV-Lys) and virus-like particles of physalis mottle virus (PhMV) in four types of soil. The particles were loaded in a cylindrical column of soil, eluted, and analyzed for the protein signal. A mathematical model was used to compare their relative mobility. Data indicate that TMGMV has higher soil mobility compared to the other plant virus-based nanoparticles analyzed, and this appeared to be independent of the soil environment. Data indicate that the presence of a highdensity lysine corona may not be favorable for soil applications. While this work provides insights into nanoparticle design rules for soil applications, data also highlight that more systemic studies are needed to delineate the design rules for soil delivery of nanocarriers.

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Section: Resultssupporting

confidence: 89%

Section: ■ Results and Discussionmentioning

confidence: 99%

Plant Viral Nanocarrier Soil Mobility as a Function of Soil Type and Nanoparticle Properties

Venkateswaran¹,

Caparco²,

González-Gamboa³

et al. 2023

ACS Agric. Sci. Technol.

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show abstract

“…Besides, BCA is simpler, ease of use, takes less time for the experiment, requires fewer instruments, high sensitivity, and tolerance of interfering species, e.g. common surfactants 15 – 17 . The findings revealed that the protein contents analyzed by BCA in the current investigation were equivalent to those found in the prior study utilizing the Kjeldahl method.…”

Section: Resultsmentioning

confidence: 99%

Skin irritation and potential antioxidant, anti-collagenase, and anti-elastase activities of edible insect extracts

Yeerong

Sriyab

Somwongin

et al. 2021

Sci Rep

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This study aimed to investigate antioxidant, anti-aging, and irritation properties of Thai edible insect extracts, including Bombyx mori, Omphisa fuscidentalis, Euconocephalus sp., Patanga succincta, Acheta domesticus, and Lethocerus indicus. Insects were extracted by 2 different methods, including maceration using ethanol or hexane and digestion using DI water. Then the extracts were determined for protein content using bicinchoninic acid assay and antioxidant activities using 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid), 2,2-diphenyl-1-picrylhydrazyl, ferric reducing antioxidant power, and ferric thiocyanate assays. Anti-aging activities were investigated by determination of collagenase and elastase inhibitory activities using spectrophotometric assay. Maceration by hexane yielded the highest extract content, whereas aqueous extract from digestion possessed the significantly highest protein content and biological activities (p < 0.05). Interestingly, aqueous extracts of A. domesticus possessed the significantly highest biological activities (p < 0.05) with Trolox equivalent antioxidant capacity value of 8.8 ± 0.1 mmol Trolox/mg, DPPH· inhibition of 19.5 ± 3.8%, equivalent concentration of 12.1 ± 0.7 µM FeSO4/mg, lipid peroxidation inhibition of 31.3 ± 2.4%, collagenase inhibition of 60.8 ± 2.1%, elastase inhibition of 17.0 ± 0.1%, and no irritation effect on chorioallantoic membrane and volunteers. Therefore, aqueous extract of A. domesticus would be suggested for further topical product development.

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“…25 Notably, BCA method is used to determine the concentration of BSA and CCCH-ZAP. 26 BCA protein quantication kit was used to achieve rapid, stable and sensitive concentration determination of protein. The principle of this kit is that the peptide bond structure in the protein molecule can form a complex with Cu 2+ in an alkaline environment and reduce Cu 2+ to Cu + , while BCA reagent can sensitively and specically bind to Cu + to form a stable and colored complex and has a maximum absorption value at 562 nm.…”

Section: Determination Of the Encapsulation Efficiency (Ee)mentioning

confidence: 99%

Assembly encapsulation of BSA and CCCH-ZAP in the sodium alginate/atractylodis macrocephalae system

Zhang

Fan

et al. 2022

RSC Adv.

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Pandora box of BCA assay. Investigation of the accuracy and linearity of the microplate bicinchoninic protein assay: Analytical challenges and method modifications to minimize systematic errors

Cited by 26 publications

References 10 publications

Plant Viral Nanocarrier Soil Mobility as a Function of Soil Type and Nanoparticle Properties

Plant Viral Nanocarrier Soil Mobility as a Function of Soil Type and Nanoparticle Properties

Skin irritation and potential antioxidant, anti-collagenase, and anti-elastase activities of edible insect extracts

Assembly encapsulation of BSA and CCCH-ZAP in the sodium alginate/atractylodis macrocephalae system

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