Assembly encapsulation of BSA and CCCH-ZAP in the sodium alginate/atractylodis macrocephalae system

Zhang, Shuxin; Fan, Hai; Yi, Chunrong; Liu, Ying; Yang, Kunmei; Liu, Shenglong; Cheng, Ziqiang; Sun, Jianchao

doi:10.1039/d2ra01767a

Cited by 3 publications

(1 citation statement)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…FT-IR analysis was used to identify the functional groups present within the MPs. As shown in Figure 5 , the spectrum of BSA/ALG MPs ( Figure 5 a) indicated that the peak observed at 3356 cm −1 corresponded to an O–H group [ 51 ], and this O–H stretching peak has also been reported for BSA encapsulated in alginate [ 52 ]. This result suggested that BSA was indeed encapsulated within alginate MPs.…”

Section: Resultsmentioning

confidence: 91%

Physical, Chemical, and Biological Properties of Chitosan-Coated Alginate Microparticles Loaded with Porcine Interleukin-1β: A Potential Protein Adjuvant Delivery System

Chen

Lien

et al. 2022

IJMS

View full text Add to dashboard Cite

We previously developed chicken interleukin-1β (IL-1β) mutants as single-dose adjuvants that induce protective immunity when co-administered with an avian vaccine. However, livestock such as pigs may require a vaccine adjuvant delivery system that provides long-lasting protection to reduce the need for successive booster doses. Therefore, we developed chitosan-coated alginate microparticles as a carrier for bovine serum albumin (BSA) or porcine IL-1β (pIL-1β) and assessed their physical, chemical, and biological properties. Electrospraying of the BSA-loaded alginate microparticles (BSA/ALG MPs) resulted in an encapsulation efficiency of 50%, and those MPs were then coated with chitosan (BSA/ALG/CHI MPs). Optical and scanning electron microscopy, zeta potential analysis, and Fourier transform infrared spectroscopy were used to characterize these MPs. The BSA encapsulation parameters were applied to ALG/CHI MPs loaded with pIL-1β, which were not cytotoxic to porcine fibroblasts but had enhanced bio-activity over unencapsulated pIL-1β. The chitosan layer of the BSA/ALG/CHI MPs prevented burst release and facilitated sustained release of pIL-1β for at least 28 days. In conclusion, BSA/ALG/CHI MPs prepared as a carrier for pIL-1β may be used as an adjuvant for the formulation of pig vaccines.

show abstract

Section: Resultsmentioning

confidence: 91%

Physical, Chemical, and Biological Properties of Chitosan-Coated Alginate Microparticles Loaded with Porcine Interleukin-1β: A Potential Protein Adjuvant Delivery System

Chen

Lien

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

A highly selective “on–off” fluorescent sensor for detection of Fe3+ ion in protein and aqueous media: Synthesis, structural characterization, and computational studies

Kouser

Khan

Arjmand

et al. 2023

Inorganica Chimica Acta

View full text Add to dashboard Cite

Effect of polysaccharides on conformational changes and functional properties of protein-polyphenol binary complexes: A comparative study

Zhang,

Li,

Zhang

et al. 2023

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

Assembly encapsulation of BSA and CCCH-ZAP in the sodium alginate/atractylodis macrocephalae system

Abstract: ZAP and BSA can be encapsulated in the sodium alginate/atractylodis macrocephalae system using an assembly method.

Cited by 3 publications

References 39 publications

Physical, Chemical, and Biological Properties of Chitosan-Coated Alginate Microparticles Loaded with Porcine Interleukin-1β: A Potential Protein Adjuvant Delivery System

Physical, Chemical, and Biological Properties of Chitosan-Coated Alginate Microparticles Loaded with Porcine Interleukin-1β: A Potential Protein Adjuvant Delivery System

A highly selective “on–off” fluorescent sensor for detection of Fe3+ ion in protein and aqueous media: Synthesis, structural characterization, and computational studies

Effect of polysaccharides on conformational changes and functional properties of protein-polyphenol binary complexes: A comparative study

Contact Info

Product

Resources

About