Palmitoyl-CoA effect on cytochrome c release, a key process of apoptosis, from liver mitochondria of rat with sucrose diet-induced obesity

Barrios-Maya, Miguel-Angel; Ruiz-Ramírez, Angélica; Quezada, Héctor; Acuña, Carlos L. Céspedes; El-Hafidi, Mohammed

doi:10.1016/j.fct.2021.112351

Cited by 11 publications

(8 citation statements)

References 47 publications

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“…In fact, Bax is a pro‐apoptotic protein which can eliminate the membrane potential of mitochondria and promote the release of Cyt.c by affecting PTP. As for Bcl‐2, it is an anti‐apoptotic protein that can preserve the membrane potential and block the release of Cyt.c 23,24 . In this study, PRP significantly decreased the protein levels of Bax and Cyt.c while notably increased the protein levels of Bcl‐2 in UVB‐irradiated HaCaT cells.…”

Section: Discussionsupporting

confidence: 49%

“…As for Bcl-2, it is an anti-apoptotic protein that can preserve the membrane potential and block the release of Cyt.c. 23,24 In this study, PRP significantly decreased the protein levels of Bax and Cyt.c while notably thereby modifying or regulating the folding of proteins. 31 CHOP is a specific mediator for the ER stress-induced apoptosis pathway.…”

Section: Discussionmentioning

confidence: 53%

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Platelet‐rich plasma protects human keratinocytes from UVB‐induced apoptosis by attenuating inflammatory responses and endoplasmic reticulum stress

Duan¹,

Ren²,

Du³

et al. 2022

J of Cosmetic Dermatology

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Due to increased outdoor activities, diversified styles of clothes, prolonged human life span, and depleted ozone, people are forced to expose to stronger ultraviolet (UV) for longer time. 1,2 If the skin is exposed to sunlight for a long time, beyond the threshold of UV light that the skin can withstand, the skin can be damaged and photoaging phenomena may ensue. 3,4 Photoaging mainly refers to the process of skin aging caused by extrinsic factors such as UV light, smoking, and exposure to chemicals. In the light of a large number of

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Section: Discussionsupporting

confidence: 49%

Section: Discussionmentioning

confidence: 53%

Platelet‐rich plasma protects human keratinocytes from UVB‐induced apoptosis by attenuating inflammatory responses and endoplasmic reticulum stress

Duan¹,

Ren²,

Du³

et al. 2022

J of Cosmetic Dermatology

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“…Thus, we speculate that compound 32, by down-regulating the expression of mitochondrial cytochrome C protein, prevents normal electron transport of the respiratory chain and the accumulation of ROS, which eventually causes iron death [38]. On the other hand, cytochrome C is a key substance for mitochondria to initiate the apoptotic program [39], and apoptosis plays a vital role in maintaining the normal physiological homeostasis of the organism and removing senescent cells from the organism [40]. Reduced expression of mitochondrial cytochrome C protein inhibited the apoptotic effect and affected the normal growth and development of Plutella xylostella.…”

Section: Kegg Classification Analysismentioning

confidence: 99%

Discovery of a Novel Class of Acylthiourea-Containing Isoxazoline Insecticides against Plutella xylostella

Jiang

Luo

et al. 2023

Molecules

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Isoxazoline structures are widely found in natural products and are rich in biological activities. This study discloses the development of a series of novel isoxazoline derivatives by introducing acylthiourea fragments to access insecticidal activity. All synthetic compounds were examined for their insecticidal activity against Plutella xylostella, with results showing moderate to strong activity. Based on this, the structure–activity relationship analysis was carried out via the constructed three-dimensional quantitative structure–activity relationship model to further guide the structure optimization, resulting in the optimal compound 32. The LC50 of compound 32 against Plutella xylostella was 0.26 mg/L, demonstrating better activity than the positive control, ethiprole (LC50 = 3.81 mg/L), avermectin (LC50 = 12.32 mg/L), and compounds 1–31. The insect GABA enzyme-linked immunosorbent assay demonstrated that compound 32 might act on the insect GABA receptor, and the molecular docking assay further illustrated the mode of action of compound 32 with the GABA receptor. In addition, the proteomics analysis indicated that the action of compound 32 on Plutella xylostella was multi-pathway.

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“…Dysregulation of uptake, utilization, and/or storage of triglycerides and FFA [ 4 , 5 ] leads to accumulation of bioactive molecules such as acyl-CoA and ceramides [ 6 , 7 ] triggering pathways including mitogen-activated protein kinase (MAPK), extracellular-signal-regulated kinase (ERK), sterol regulatory element binding protein-1c (SREBP-1c), and uncoupling protein 2 (UCP2) or promoting release of intracellular Ca storages [ 8–11 ]. Apart from diabetes research, lipotoxicity plays a role in the pathogenesis of non-alcoholic fatty liver disease [ 12 ], cardiovascular disease [ 13 ], and apoptosis promoted by cytochrome C release [ 14 ]. Therefore, establishment of standardized protocols for in vitro models with defined FFA as lipid stressors is required for the investigation of underlying mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Preparation of fatty acid solutions exerts significant impact on experimental outcomes in cell culture models of lipotoxicity

Römer

Rawat

Linn

et al. 2021

Biology Methods and Protocols

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Free fatty acids are essentially involved in the pathogenesis of chronic diseases such as diabetes mellitus, non-alcoholic fatty liver disease, and cardiovascular disease. They promote mitochondrial dysfunction, oxidative stress, respiratory chain uncoupling, endoplasmic reticulum-stress and modulate stress-sensitive pathways. These detrimental biological effects summarized as lipotoxicity mainly depend on fatty acid carbon chain length, degree of unsaturation, concentration, and treatment time. Preparation of fatty acid solutions involves dissolving and complexing. Solvent toxicity and concentration, the amount of bovine serum albumin, and the ratio of albumin to fatty acids can vary significantly between equal concentrations, mediating considerable harmful effects and/or interference with certain assays such as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Herein, we studied the impact of commonly used solvents ethanol and dimethyl sulfoxide, varying concentrations of bovine serum albumin, treatment duration, and respectively prepared oleic acid solutions on MTT to formazan conversion, adenosine triphosphate level, and insulin content and secretion of murine β-cell line MIN6. Our data show that experimental outcomes and assay readouts can be significantly affected by mere preparation of fatty acid solutions and should thus be carefully considered and described in detail to ensure comparability and distinct evaluation of data.

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Palmitoyl-CoA effect on cytochrome c release, a key process of apoptosis, from liver mitochondria of rat with sucrose diet-induced obesity

Cited by 11 publications

References 47 publications

Platelet‐rich plasma protects human keratinocytes from UVB‐induced apoptosis by attenuating inflammatory responses and endoplasmic reticulum stress

Platelet‐rich plasma protects human keratinocytes from UVB‐induced apoptosis by attenuating inflammatory responses and endoplasmic reticulum stress

Discovery of a Novel Class of Acylthiourea-Containing Isoxazoline Insecticides against Plutella xylostella

Preparation of fatty acid solutions exerts significant impact on experimental outcomes in cell culture models of lipotoxicity

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