Platelet‐rich plasma protects human keratinocytes from UVB‐induced apoptosis by attenuating inflammatory responses and endoplasmic reticulum stress

Duan, Guang‐Hua; Ren, Zhao-Qi; Du, Bin; Wen, Shifeng; Dong, Haijiao; Du, Ai-Cui

doi:10.1111/jocd.15559

Cited by 6 publications

(6 citation statements)

References 30 publications

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“…The effect was mediated by upregulated extracellular signal-regulated kinase cascade 1/2 (ERK1/2) and protein kinase B (Akt) signal pathways, with reduced intracellular oxidation [ 67 ]. Such observations join other reports unrelated to reproductive biology [ 5 , 61 , 67 ] where metabolic spoliation was erased following PRP dosing. The question of similar PRP effects in the adult human ovary seems answered, for now, only by small series and case reports [ 42 , 43 , 44 ].…”

Section: Prp and Organ Damage Reversalsupporting

confidence: 88%

“…One major event during apoptosis is the escape of cytochrome-C (cyt-C), a highly conserved mitochondrial hemeprotein vital to electron transport and cellular respiration [ 60 ]. Transit of cyt-C into cytosol can occur through a membrane channel controlled by the first regulator of apoptosis to be identified in any organism, Bcl-2.61 Bcl-2 thus exerts an anti-apoptotic function by conserving membrane potential via blocking release of cyt-C [ 61 , 62 ]. While PRP can induce proliferation of Bcl-2 with therapeutic effects resulting from significant declines in cyt-C [ 61 ], this awaits full characterization in the adult human ovary.…”

Section: Subcellular Characteristics After Prpmentioning

confidence: 99%

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Multichannel Recovery Potential with Activated Autologous Intraovarian Platelet-Rich Plasma and Its Derivatives

Sills

Wood

2023

Medicines

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Platelet-rich plasma (PRP) is an ‘orthobiologic’ with recognized roles in plastic surgery, musculoskeletal disorders, dentistry, dermatology, and more recently, ‘ovarian rejuvenation’. Intraovarian PRP involves a complex secretome discharged after platelet activation, comprising multiple cytokine mediators delivered surgically to older or inactive ovarian tissue. Loss of oocyte meiotic fidelity and impaired fertilization accompanying advanced maternal age are already managed by IVF, but only with eggs provided by younger donors. However, if the observed effect of rectifying embryo ploidy error can be proven beyond case reports and small series, activated PRP (or its condensed plasma cytokines) would deliver a welcome therapeutic disruption that is difficult to overstate. Because shortcomings in ovarian function are presently addressed mainly by pharmacological approaches (i.e., via recombinant gonadotropins, GnRH analogs, or luteal support), autologous PRP would represent an unusual departure from these interventions. Given the diversity of platelet cargo proteins, the target response of intraovarian PRP is probably not confined to oocytes or follicles. For example, PRP manipulates signal networks driving improved perfusion, HOX regulation, N-glycan post-translational modification, adjustment of voltage-gated ion channels, telomere stabilization, optimization of SIRT3, and ribosome and mitochondria recovery in older oocytes. While multichannel signals operating on various pathways are not unique to reproductive biology, in intraovarian PRP this feature has received little study and may help explain why its standardization has been difficult. Against this background, our report examines the research themes considered most likely to shape clinical practice.

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Section: Prp and Organ Damage Reversalsupporting

confidence: 88%

Section: Subcellular Characteristics After Prpmentioning

confidence: 99%

Multichannel Recovery Potential with Activated Autologous Intraovarian Platelet-Rich Plasma and Its Derivatives

Sills

Wood

2023

Medicines

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“…Here, to validate the synergistic anti-tumor efficacy of TET-CTM/L, all celastrol treatments including CEL, CEL+TET, CTM, and TET/L were taken as controls, our study evaluated the cytotoxicity of TET-CTM/L against HepG2 cells. 41 , 42 All various celastrol treatments had obvious proliferation inhibition of HepG2 cells, meanwhile we found that the intensity of proliferation inhibition increased significantly with the increase in celastrol concentration after treatment for 24 and 48 h ( Figure 3C and D ). The IC 50 values of various celastrol treatments against HepG2 cells for 24 h were 3.31 ± 0.64, 2.99 ± 0.48, 9.04 ± 0.88, 34.47 ± 1.63, 8.89 ± 0.55 μg/mL, respectively.…”

Section: Resultsmentioning

confidence: 85%

Sequentially Released Liposomes Enhance Anti-Liver Cancer Efficacy of Tetrandrine and Celastrol-Loaded Coix Seed Oil

Chen,

Zhang,

Qian

et al. 2024

IJN

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Background A sequential release co-delivery system is an effective strategy to improve anti-cancer efficacy. Herein, multicomponent-based liposomes (TET-CTM/L) loaded with tetrandrine (TET) and celastrol (CEL)-loaded coix seed oil microemulsion (CTM) were fabricated, which showed synergistic anti-liver cancer activities. By virtue of Enhanced Permeability and Retention (EPR) effect, TET-CTM/L can achieve efficient accumulation at the tumor site. TET was released initially to repair abnormal vessels and decrease the fibroblasts, and CTM was released subsequently for eradication of tumor tissue. Methods TEM (transmission electron microscopy) and DLS (dynamic light scattering) were adopted to characterize the TET-CTM/L. Flow cytometry was adopted to examine the cellular uptake and cytotoxicity of HepG2 cells. The HepG2 xenograft nude mice were adopted to evaluate the anti-tumor efficacy and systemic safety of TET-CTM/L. Results TEM images of TET-CTM/L showed the structure of small particle size of CTM within large-size liposomes, indicating that CTM can be encapsulated in liposomes by film dispersion method. In in vitro studies, TET-CTM/L induced massive apoptosis toward HepG2 cells, indicating synergistic cytotoxicity against HepG2 cells. In in vivo studies, TET-CTM/L displayed diminished systemic toxicity compared to celastrol or TET used alone. TET-CTM/L showed the excellent potential for tumor-targeting ability in a biodistribution study. Conclusion Our study provides a new strategy for combining anti-cancer therapy that has good potential not only in the treatment of liver cancer but also can be applied to the treatment of other solid tumors.

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“…RNAseq further identified the downregulated genes related to cell replication [33]. In contrast, some found that platelet-rich plasma increased the proliferation of human HaCaT keratinocytes [34], while others found no relevant change in HaCaT proliferation [35]. Considering dentistry, platelet-rich stimulated the proliferation of SCC25 epithelial-like cells originally isolated from the tongue of a patient with squamous cell carcinoma [14], while PRF decreased the viable cell number using an MTT assay of an oral epithelial cell line derived from a gingival carcinoma and metastasis to the cervical lymph node [36].…”

Section: Discussionmentioning

confidence: 99%

PRF Lysates Enhance the Proliferation and Migration of Oral Squamous Carcinoma Cell Lines

Panahipour,

Croci,

Guarnieri

et al. 2023

Dentistry Journal

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Platelet-rich fibrin (PRF) is an autologous fibrin-rich matrix where activated platelets and leucocytes accumulate. PRF has a wide spectrum of clinical indications with the overall aim of supporting tissue regeneration which in dentistry includes the healing of healthy oral mucosa with epithelial cells. In oral squamous cell carcinoma lesions, however, epithelial cells undergo malignant transformation, indicated by their unrestricted proliferation and migration potential, which should not be further enhanced by a wound-healing formula. Yet, little is known about how oral squamous cell carcinomas respond to PRF lysates. The aim of the present study was, therefore, to test the capacity of PRF lysates to change the transcriptome of HSC2 oral squamous carcinoma cells and perform bioassays to support the findings. Based on the RNAseq analysis, PRF lysates caused an increase in the genes functionally linked to cell replication and migration. In support of this screening approach, PRF lysates enhanced the proliferation of HSC2 oral squamous carcinoma cells, as indicated by 3[H]-thymidine incorporation, cell counting, and the expression of proliferation-related genes. Moreover, PRF lysates sped up cell migration in a scratch assay requiring actin polymerization. Taken together, our data showing that PRF lysates are mitogenic and stimulate motility of oral squamous carcinoma cell lines could be an indication that treatment with PRF in cases of oral carcinoma should be carefully considered.

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Platelet‐rich plasma protects human keratinocytes from UVB‐induced apoptosis by attenuating inflammatory responses and endoplasmic reticulum stress

Cited by 6 publications

References 30 publications

Multichannel Recovery Potential with Activated Autologous Intraovarian Platelet-Rich Plasma and Its Derivatives

Multichannel Recovery Potential with Activated Autologous Intraovarian Platelet-Rich Plasma and Its Derivatives

Sequentially Released Liposomes Enhance Anti-Liver Cancer Efficacy of Tetrandrine and Celastrol-Loaded Coix Seed Oil

PRF Lysates Enhance the Proliferation and Migration of Oral Squamous Carcinoma Cell Lines

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