p53 Protein Expression in Benign and Malignant Breast Lesions

Ioakim-Liossi, Anne; Markopoulos, Christos; Karakitsos, Peter; Safioleas, Michael; Gogas, J; Vaiopoulos, George

doi:10.1159/000331968

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…Evidences observed in these selected studies, showed that the p53 analyses among women with BBD are still scarce. For example, atypic hyperplasias group was poorly represented in selected studies, regardless their high-risk for breast cancer development (Eriksson et al, 1994;Millikan et al, 1995;Wells et al, 1995;Younes et al, 1995;Ioakim-Liossi et al, 1998;Feakins et al, 1999;Kandel et al, 2000;Niezabitowski et al, 2001;Ryška et al, 2001;Herbert et al, 2002;Selim et al, 2002;Angèle et al, 2004;Sirotkovic-Skerlev et al, 2005;Khalifeh et al, 2008;Shin et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…A limitation observed was that not all studies described any benign breast disease type or group lesion (Eriksson et al, 1994;Millikan et al, 1995;Ioakim-Liossi et al, 1998;Ryška et al, 2001;Herbert et al, 2002;Angèle et al, 2004;Sirotkovic-Skerlev et al, 2005;Khalifeh et al, 2008;Shin et al, 2009), what implicates in difficulties in (Feakins et al, 1999;Niezabitowski et al, 2001), showing great differences between p53 expression in totally benign, borderline and malignant lesions. Such distinctions may be a reflection of biopsy techniques and different forms of BBD classification according to pathologist's perspective, which can lead to differences in the findings (O'Flynn et al, 2010;Simpson et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

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P53 Expression in benign Breast Disease Development: A Systematic Review

Costa

Silva

2020

Asian Pac J Cancer Prev

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Background: Benign breast disease (BBD) is one of main breast cancer risk factors. Dysfunctions on p53 protein, which has a genome protective role, have been related to breast cancer developments. However, its role on BBD development is still unclear. Methods: A systematic review of literature was proceeded according to PRISMA-P guidelines. PubMed, BVS, MEDLINE and Scholar Google were used as databases, complemented by a manual search in articles references. Articles searches were conducted from May to July 2019 and publications in English, Spanish and Portuguese were selected. P53 expression was set as outcome among women with BBD and were included only articles with good quality according STROBE tools. Data concerning p53 expression frequencies were independently extracted by two review authors, and eligible articles were synthesized. Results: From 12 studies selected for this review, the majority analyzed p53 expression in non-proliferative lesions and general p53 expressions ranged from 0 to 100%. P53 expression was more frequently observed in cases series studies (91.7%) and in studies conducted in Occidental Europe (41.7%). P53 expression was more frequent among tissues with fibrocystic disease (22.5%) and fibroadenoma (22.5%). Conclusion: When compared with all breast tissues types, benign breast disease corresponds to 34.39% of p53 expression. Second outcomes were not evaluated because the heterogeneity observed in selected studies. In addition, more studies considering ethnicity and benign breast disease classification should also be considered for further analysis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

P53 Expression in benign Breast Disease Development: A Systematic Review

Costa

Silva

2020

Asian Pac J Cancer Prev

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“…p53 activity is abrogated in half of all breast cancers [168,169]. pRb is mutated in -25% of tumor cells [170].…”

Section: Discussionmentioning

confidence: 99%

Analysis of Breast Cell-Lineage Response Differences to Taxol Using a Novel Co-Culture System

Gollahon¹,

Collie²

2005

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Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Department of Defense, Washington Headquarters Services, Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. Email: ]auren.gollahon@ttu.edu REPORT DATE PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION REPORT NUMBERTexas Tech University Lubbock, TX 79409 SPONSORING / MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR'S ACRONYM(S) U.S. Army Medical Research and Materiel Command Fort Detrick, Maryland 21702-5012 SPONSOR/MONITOR'S REPORT NUMBER(S) DISTRIBUTION / AVAILABILITY STATEMENTApproved for Public Release; Distribution Unlimited SUPPLEMENTARY NOTESOriginal contains colored plates: ALL DTIC reproductions will be in black and white.14. ABSTRACT We have established a new co-culture system in which human mammary epithelial cells (HMEC) and human mammary tumor cells (HMT) are physically grown together. We hypothesized that cells in co-culture (CC) would generate gene expression profiles different from homogeneous cell populations. In this study, cells were incubated with blue or red CellTracker Dyes and co-cultured. Our novel capture system allowed cells to be co-cultured and then quickly separated while maintaining -90% viability. Using deconvolution microscopy, co-cultured HMEC were observed to form focal, gland-like structures surrounded by TTUderived from an invasive ductal carcinoma. Using a differential trypsinization technique, cell populations were rapidly separated to perform RNA extractions performed (<2 h) in order to obtain expression profiles from still viable cells. RT2 profiler PCR Array (SuperArray) RT-PCR was utilized to analyze differential gene expression between parent cell lines and cells co-cultured. We observed that in co-culture HMEC become more cancerous compared to homogenous parent HMEC and CC -TTUexhibit gene expression profiles considered to be less cancerous that that of parent HMT. Replated CC HMEC have both gene expression profiles of CC HMEC and parent HMEC, but were more similar to CC HMEC. Replated TTU showed similar results. DAMD1 7-02-1-0581 SUBJECTGollahon, Lauren, S. Gollahon, Lauren, S. INTRODUCTION:The subject of this research is investigating whether different normal cell types, isolated from primary breast tissue 1) respond differently in co-cul...

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