P34HB film promotes cell adhesion, in vitro proliferation, and in vivo cartilage repair

Abstract

The management of chondral defects is a challenging topic of current interest for scientists and surgeons, which has a crucial impact on human cost. Even after several centuries after its first observation, this problem has still not found a satisfactory and definitive answer. Cartilage tissue engineering, which involves novel natural scaffolds, has emerged as a promising strategy for cartilage regeneration and repair. In this study, bio-plasticpoly-3-hydroxybutyrate-4-hydroxybutyrate (P34HB) film was first fabricated. The characteristics of P34HB film were tested using SEM and AFM. Cell morphologies on P34HB film were obtained using SEM and fluorescence microscopy after cell seeding. The tests of cell adhesion and proliferation on P34HB film were conducted using MTT and CCK-8 assays, respectively. Furthermore, full cartilage defects in rats were created and P34HB films were implanted to evaluate their healing effects within 8 weeks. It was found that P34HB film, as a biomaterial implant, possessed good in vitro properties for cell adhesion, migration, and proliferation. Importantly, in the in vivo experiment, P34HB film exhibited desirable healing outcomes. These results demonstrated that P34HB film was a good scaffold for cartilage tissue engineering for improving cell proliferation and adhesion.

Electrospun Poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/Graphene Oxide Scaffold: Enhanced Properties and Promoted in Vivo Bone Repair in Rats

“…Variable-controlling method was adopted to readjust technique parameters based on our previous study on pure P34HB. 24 Fabrication was performed in a hood with dry air convection (23−27 °C, 35−45% relative humidity). The electrospun solution was pumped by a 10 mL syringe with a 21-G metallic needle (0.81 and 0.54 mm for outer and internal diameter).…”

“…23−27 Our group also conducted studies on its properties in different fields. 24,25,28,29 Fu et al compared two types of P34HB (the electrospun scaffold and deposited film) and proved that the scaffold held better cellular performance in adipose-derived stem cells (ASCs). 28 Additionally, cellular behaviors on the deposited films and their capacity of in vivo cartilage regeneration were also evaluated and confirmed.…”

“…28 Additionally, cellular behaviors on the deposited films and their capacity of in vivo cartilage regeneration were also evaluated and confirmed. 24 Moreover, Li et al further proved that a cell-seeded electrospun P34HB/TGF-β1 scaffold could promote in vivo cartilage repair in rabbits. 25 However, to the best of our knowledge, the performance of this material in BTE application has barely been explored and its bone repair potential has not been confirmed with in any animal models in vivo.…”

“…The latest generation of polyhydroxyalkanoate (PHA) family, poly­(3-hydroxybutyrate- co -4-hydroxybutyrate) copolymer (P34HB), has attracted more attention in the biomedical field than its predecessors like poly­(3-hydroxybutyrate- co -3-hydroxybutyrate) (PHBV) and polyhydroxybutyrate (PHB). , So far, studies about P34HB have mainly focused on the central nervous system, cartilage, and cardiovascular tissues. − Our group also conducted studies on its properties in different fields. ,,, Fu et al compared two types of P34HB (the electrospun scaffold and deposited film) and proved that the scaffold held better cellular performance in adipose-derived stem cells (ASCs) . Additionally, cellular behaviors on the deposited films and their capacity of in vivo cartilage regeneration were also evaluated and confirmed . Moreover, Li et al further proved that a cell-seeded electrospun P34HB/TGF-β1 scaffold could promote in vivo cartilage repair in rabbits .…”

PCL‐PEG‐PCL film promotes cartilage regeneration in vivo

“…Biomimetic scaffolds using natural and synthetic biomaterials are highly desirable to recreate the micro‐architecture of articular cartilage. Some studies have suggested that environmental, cytokines and suitable scaffold materials can promote bone and cartilage tissue regeneration . Although intensive research has focused on the developmental biology and regeneration of cartilage tissue, and a diverse plethora of biomaterials have been developed for this purpose, cartilage regeneration is still suboptimal with disadvantages, such as lack of a layered structure, mechanical mismatch with native cartilage, and inadequate integration between native tissue and the implanted scaffold …”